Recombinant Human His6-Ubiquitin Protein, CF | R&D Systems U-530-02M product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human His6-Ubiquitin Protein, CF

catalog :

U-530-02M

quantity :

2 mg

price :

120 USD

citations: 15

Reference
Lao L, Bourdeau I, Gagliardi L, He X, Shi W, Hao B, et al. ARMC5 is part of an RPB1-specific ubiquitin ligase implicated in adrenal hyperplasia. Nucleic Acids Res. 2022;: pubmed publisher
Pirooznia S, Wang H, Panicker N, Kumar M, Neifert S, Dar M, et al. Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease. Sci Adv. 2022;8:eabh1824 pubmed publisher
Wang S, Wang R, Peralta C, Yaseen A, Pavletich N. Structure of the FA core ubiquitin ligase closing the ID clamp on DNA. Nat Struct Mol Biol. 2021;28:300-309 pubmed publisher
Seo H, Kim H, Yoon J, Kweon T, Park Y, Kang J, et al. Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion. Cell Death Dis. 2020;11:815 pubmed publisher
Ma P, Song N, Li Y, Zhang Q, Zhang L, Zhang L, et al. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning. Cell Rep. 2019;28:541-553.e4 pubmed publisher
Yip M, Keszei A, Feng Q, Chu V, McKenna M, Shao S. Mechanism for recycling tRNAs on stalled ribosomes. Nat Struct Mol Biol. 2019;26:343-349 pubmed publisher
Wu R, Semlow D, Kamimae Lanning A, Kochenova O, Chistol G, Hodskinson M, et al. TRAIP is a master regulator of DNA interstrand crosslink repair. Nature. 2019;567:267-272 pubmed publisher
Tucker W, Kinghorn A, Fraser L, Cheung Y, Tanner J. Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1. Int J Mol Sci. 2018;19: pubmed publisher
Yang C, Jividen K, Spencer A, Dworak N, Ni L, Oostdyk L, et al. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9. Mol Cell. 2017;66:503-516.e5 pubmed publisher
Schunter S, Villa R, Flynn V, Heidelberger J, Classen A, Beli P, et al. Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster. PLoS ONE. 2017;12:e0177408 pubmed publisher
Luo S, Jing L, Zhao T, Li Y, Liu Z, Diao A. Ubiquitination and dynactin regulate TMEPAI lysosomal trafficking. Sci Rep. 2017;7:42668 pubmed publisher
Nazio F, Carinci M, Valacca C, Bielli P, Strappazzon F, Antonioli M, et al. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation. J Cell Biol. 2016;215:841-856 pubmed
Fullbright G, Rycenga H, Gruber J, Long D. p97 Promotes a Conserved Mechanism of Helicase Unloading during DNA Cross-Link Repair. Mol Cell Biol. 2016;36:2983-2994 pubmed publisher
Rodrigo Brenni M, Gutierrez E, Hegde R. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell. 2014;55:227-37 pubmed publisher
Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, et al. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J. 2014;28:1938-46 pubmed publisher

product information

brand :

R&D Systems

master code :

U-530

SKU :

U-530-02M

product name :

Recombinant Human His6-Ubiquitin Protein, CF

description :

The Recombinant Human His6-Ubiquitin Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human His6-Ubiquitin Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin

category :

Proteins and Enzymes

sizes available :

2 mg

buffer :

2.4 mg/ml (250 µM) in 10 mM HEPES pH 7.5

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

species :

Human

theoretical molecular weight :

9.6 kDa

gene symbol :

UBB

details of functionality :

Recombinant Human His6-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ubiquitin concentration of 0.2-1 mM.

accessionNumbers :

P0CG47

applications :

Enzyme Activity

extended description :

This item is in process of being discontinued.

source :

E. coli-derived Recombinant Human His6-Ubiquitin Protein, CF

USD :

114

USD 2023 :

120 USD

product details :

This item is in process of being discontinued.

alt names :

RPS27A, UBA52, UBB, UBB ubiquitin B, UBC

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 24 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.