Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF | R&D Systems U-202B-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF

catalog :

U-202B-050

quantity :

50 ug

price :

400 USD

more info or order :

R&D Systems product webpage

citations: 10

Reference
Zhang S, Zou S, Yin D, Zhao L, Finley D, Wu Z, et al. USP14-regulated allostery of the human proteasome by time-resolved cryo-EM. Nature. 2022;605:567-574 pubmed publisher
Patchett S, Lv Z, Rut W, Bekes M, Drag M, Olsen S, et al. A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease. Cell Rep. 2021;36:109754 pubmed publisher
Nishiyama A, Mulholland C, Bultmann S, Kori S, Endo A, Saeki Y, et al. Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation. Nat Commun. 2020;11:1222 pubmed publisher
Rycenga H, Wolfe K, Yeh E, Long D. Uncoupling of p97 ATPase activity has a dominant negative effect on protein extraction. Sci Rep. 2019;9:10329 pubmed publisher
Liang F, Miller A, Longerich S, Tang C, Maranon D, Williamson E, et al. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nat Commun. 2019;10:2849 pubmed publisher
Chen L, Zhu G, Johns E, Yang X. TRIM11 activates the proteasome and promotes overall protein degradation by regulating USP14. Nat Commun. 2018;9:1223 pubmed publisher
Boselli M, Lee B, Robert J, Prado M, Min S, Cheng C, et al. An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons. J Biol Chem. 2017;292:19209-19225 pubmed publisher
Yamaguchi L, Nishiyama A, Misaki T, Johmura Y, Ueda J, Arita K, et al. Usp7-dependent histone H3 deubiquitylation regulates maintenance of DNA methylation. Sci Rep. 2017;7:55 pubmed publisher
Deng M, Yang X, Qin B, Liu T, Zhang H, Guo W, et al. Deubiquitination and Activation of AMPK by USP10. Mol Cell. 2016;61:614-624 pubmed publisher
Iguchi M, Kujuro Y, Okatsu K, Koyano F, Kosako H, Kimura M, et al. Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. J Biol Chem. 2013;288:22019-32 pubmed publisher

product information

master code :

U-202B

SKU :

U-202B-050

product name :

Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF

unit size :

50 ug

description :

The Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF from R&D Systems is derived from Synthetic. The Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin

category :

Proteins and Enzymes

buffer :

Supplied as a solution in MES and DMSO.

conjugate :

Vinyl Sulfone

purity :

>95%, by HPLC.

theoretical molecular weight :

8.6 kDa

details of functionality :

Recombinant Human Ubiquitin-Vinyl Sulfone added to in vitro assays will inhibit deubiquitinating enzymes. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Vinyl Sulfone concentration of 1-5 ╡M.

accessionNumbers :

P0CG47.1

applications :

Enzyme Activity

source long :

Chemically Synthesized Ubiquitin protein Met1 - Gly75

source short :

Synthetic

USD :

400 USD

alt names :

RPS27A, UBA52, UBB, UBB ubiquitin B, UBC

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage