Recombinant Human Ubiquitin Vinyl Sulfone Protein, CF | R&D Systems U-202-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Ubiquitin Vinyl Sulfone Protein, CF

catalog :

U-202-050

quantity :

50 ug

price :

323 USD

citations: 11

Reference
Zhang S, Zou S, Yin D, Zhao L, Finley D, Wu Z, et al. USP14-regulated allostery of the human proteasome by time-resolved cryo-EM. Nature. 2022;605:567-574 pubmed publisher
Patchett S, Lv Z, Rut W, Bekes M, Drag M, Olsen S, et al. A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease. Cell Rep. 2021;36:109754 pubmed publisher
Nishiyama A, Mulholland C, Bultmann S, Kori S, Endo A, Saeki Y, et al. Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation. Nat Commun. 2020;11:1222 pubmed publisher
Rycenga H, Wolfe K, Yeh E, Long D. Uncoupling of p97 ATPase activity has a dominant negative effect on protein extraction. Sci Rep. 2019;9:10329 pubmed publisher
Liang F, Miller A, Longerich S, Tang C, Maranon D, Williamson E, et al. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nat Commun. 2019;10:2849 pubmed publisher
Chen L, Zhu G, Johns E, Yang X. TRIM11 activates the proteasome and promotes overall protein degradation by regulating USP14. Nat Commun. 2018;9:1223 pubmed publisher
Boselli M, Lee B, Robert J, Prado M, Min S, Cheng C, et al. An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons. J Biol Chem. 2017;292:19209-19225 pubmed publisher
Yamaguchi L, Nishiyama A, Misaki T, Johmura Y, Ueda J, Arita K, et al. Usp7-dependent histone H3 deubiquitylation regulates maintenance of DNA methylation. Sci Rep. 2017;7:55 pubmed publisher
Wang X, Mazurkiewicz M, Hillert E, Olofsson M, Pierrou S, Hillertz P, et al. The proteasome deubiquitinase inhibitor VLX1570 shows selectivity for ubiquitin-specific protease-14 and induces apoptosis of multiple myeloma cells. Sci Rep. 2016;6:26979 pubmed publisher
Deng M, Yang X, Qin B, Liu T, Zhang H, Guo W, et al. Deubiquitination and Activation of AMPK by USP10. Mol Cell. 2016;61:614-624 pubmed publisher
Iguchi M, Kujuro Y, Okatsu K, Koyano F, Kosako H, Kimura M, et al. Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. J Biol Chem. 2013;288:22019-32 pubmed publisher

product information

brand :

R&D Systems

master code :

U-202

SKU :

U-202-050

product name :

Recombinant Human Ubiquitin Vinyl Sulfone Protein, CF

description :

The Recombinant Human Ubiquitin Vinyl Sulfone Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Ubiquitin Vinyl Sulfone Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin

category :

Proteins and Enzymes

sizes available :

50 ug

buffer :

2.15 mg/ml (250 µM) in 50 mM MES, pH 6.0

conjugate :

Vinyl Sulfone

purity :

>98%, by HPLC.

species :

Human

theoretical molecular weight :

8.6 kDa

gene symbol :

UBB

details of functionality :

Add Recombinant Human Ubiquitin-Vinyl Sulfone to in vitro assays to inhibit deubiquitinating enzymes. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Vinyl Sulfone concentration of 1-5 µM.

accessionNumbers :

P0CG47

applications :

Enzyme Activity

source :

E. coli-derived Recombinant Human Ubiquitin Vinyl Sulfone Protein, CF

USD :

323 USD

alt names :

RPS27A, UBA52, UBB, UBB ubiquitin B, UBC

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.