This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
ELISA/assay
product name :
Porcine TNF-alpha Quantikine ELISA Kit
catalog :
PTA00
quantity :
1 Kit
price :
655 USD
citations: 86
Reference
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Deng Z, Jang K, Jalukar S, Du X, Kim S. Efficacy of Feed Additive Containing Bentonite and Enzymatically Hydrolyzed Yeast on Intestinal Health and Growth of Newly Weaned Pigs under Chronic Dietary Challenges of Fumonisin and Aflatoxin. Toxins (Basel). 2023;15: pubmed publisher
Changsan N, Sawatdee S, Suedee R, Chunhachaichana C, Srichana T. Aqueous cannabidiol β-cyclodextrin complexed polymeric micelle nasal spray to attenuate in vitro and ex vivo SARS-CoV-2-induced cytokine storms. Int J Pharm. 2023;640:123035 pubmed publisher
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Rissel R, Gosling M, Kamuf J, Renz M, Ruemmler R, Ziebart A, et al. Levosimendan Ameliorates Cardiopulmonary Function but Not Inflammatory Response in a Dual Model of Experimental ARDS. Biomedicines. 2022;10: pubmed publisher
Vilahur G, Sutelman P, Ben Aicha S, Mendieta G, Radik xe9 M, Schoch L, et al. Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI. Front Pharmacol. 2022;13:891801 pubmed publisher
Holanda D, Kim S. Impacts of weaning weights and mycotoxin challenges on jejunal mucosa-associated microbiota, intestinal and systemic health, and growth performance of nursery pigs. J Anim Sci Biotechnol. 2022;13:43 pubmed publisher
Rodríguez Sorrento A, Castillejos L, López Colom P, Cifuentes Orjuela G, Moreno Muñoz J, Martín Orúe S. Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model. Front Microbiol. 2022;13:831737 pubmed publisher
Kasztura M, Kiczak L, Pas x142 awska U, Bania J, Janiszewski A, Tomaszek A, et al. Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model. Int J Mol Sci. 2022;23: pubmed publisher
Lee H, Kim H, Jo C, Bok E, Kim S, Lee S, et al. IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application. Biomed Res Int. 2021;2021:4604856 pubmed publisher
Kamuf J, Garcia Bardon A, Ziebart A, Ruemmler R, Schwab J, Dib M, et al. Influence of rosuvastatin treatment on cerebral inflammation and nitro-oxidative stress in experimental lung injury in pigs. BMC Anesthesiol. 2021;21:224 pubmed publisher
Holanda D, Kim Y, Parnsen W, Kim S. Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs. Toxins (Basel). 2021;13: pubmed publisher
Qu X, Neuhoff C, Cinar M, Pröll M, Tholen E, Tesfaye D, et al. Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells. Biology (Basel). 2021;10: pubmed publisher
Rodr xed guez Sorrento A, Castillejos L, L xf3 pez Colom P, Cifuentes Orjuela G, Rodr xed guez Palmero M, Moreno Mu xf1 oz J, et al. Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Mo. Front Microbiol. 2021;12:642549 pubmed publisher
Al Obeidallah M, Jarkovska D, Valesova L, Horak J, Jedlicka J, Nalos L, et al. SOFA Score, Hemodynamics and Body Temperature Allow Early Discrimination between Porcine Peritonitis-Induced Sepsis and Peritonitis-Induced Septic Shock. J Pers Med. 2021;11: pubmed publisher
Bhogal J, Solevåg A, O Reilly M, Lee T, Joynt C, Hornberger L, et al. Hemodynamic effects of high frequency oscillatory ventilation with volume guarantee in a piglet model of respiratory distress syndrome. PLoS ONE. 2021;16:e0246996 pubmed publisher
Kamuf J, Garcia Bardon A, Ziebart A, Frauenknecht K, Folkert K, Schwab J, et al. Experimental lung injury induces cerebral cytokine mRNA production in pigs. Peerj. 2020;8:e10471 pubmed publisher
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Holanda D, Yiannikouris A, Kim S. Investigation of the Efficacy of a Postbiotic Yeast Cell Wall-Based Blend on Newly-Weaned Pigs under a Dietary Challenge of Multiple Mycotoxins with Emphasis on Deoxynivalenol. Toxins (Basel). 2020;12: pubmed publisher
Hsu Y, Wu C, Chao C, Wei Y, Chang Y, Chen Y, et al. Hypochlorite-induced porcine model of peritoneal fibrosis through the activation of IL1β-CX3CL1-TGFβ1 signal axis. Sci Rep. 2020;10:11496 pubmed publisher
Shim G, Kim S, Cheung P, Lee T, O Reilly M, Schm xf6 lzer G. Effects of sustained inflation pressure during neonatal cardiopulmonary resuscitation of asphyxiated piglets. PLoS ONE. 2020;15:e0228693 pubmed publisher
Holanda D, Kim S. Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol. Toxins (Basel). 2020;12: pubmed publisher
Spring S, Premathilake H, DeSilva U, Shili C, Carter S, Pezeshki A. Low Protein-High Carbohydrate Diets Alter Energy Balance, Gut Microbiota Composition and Blood Metabolomics Profile in Young Pigs. Sci Rep. 2020;10:3318 pubmed publisher
Duarte M, Zhou F, Dutra W, Kim S. Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs. Anim Nutr. 2019;5:351-358 pubmed publisher
López Colom P, Castillejos L, Rodríguez Sorrento A, Puyalto M, Mallo J, Martín Orúe S. Efficacy of medium-chain fatty acid salts distilled from coconut oil against two enteric pathogen challenges in weanling piglets. J Anim Sci Biotechnol. 2019;10:89 pubmed publisher
Pischke S, Hestenes S, Johansen H, Fure H, Bugge J, Espinoza A, et al. Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model. PLoS ONE. 2019;14:e0218624 pubmed publisher
Luo Y, Xu J, Zhang C, Jiang C, Ma Y, He H, et al. Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection. Vet Res. 2019;50:48 pubmed publisher
López Colom P, Yu K, Barba Vidal E, Saco Y, Martín Orúe S, Castillejos L, et al. I-FABP, Pig-MAP and TNF-α as biomarkers for monitoring gut-wall integrity in front of Salmonella Typhimurium and ETEC K88 infection in a weaned piglet model. Res Vet Sci. 2019;124:426-432 pubmed publisher
Liu Y, Li R, Chen X, Zhi Y, Deng R, Zhou E, et al. Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway. MBio. 2019;10: pubmed publisher
Saleri R, Cavalli V, Ferrari L, Ogno G, Canelli E, Martelli P, et al. Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection. Res Vet Sci. 2019;124:263-269 pubmed publisher
Boehme S, Hartmann E, Tripp T, Thal S, David M, Abraham D, et al. PO2 oscillations induce lung injury and inflammation. Crit Care. 2019;23:102 pubmed publisher
Zhang L, Wang X, Chen S, Wang S, Tu Z, Zhang G, et al. Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways. Int J Mol Sci. 2018;19: pubmed publisher
Mikołajczyk A, Złotkowska D. Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis. Int J Mol Sci. 2018;19: pubmed publisher
Qin Q, Xu X, Wang X, Wu H, Zhu H, Hou Y, et al. Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide. Sci Rep. 2018;8:15124 pubmed publisher
Sadeghi M, Hörer T, Forsman D, Dogan E, Jansson K, Kindler C, et al. Blood pressure targeting by partial REBOA is possible in severe hemorrhagic shock in pigs and produces less circulatory, metabolic and inflammatory sequelae than total REBOA. Injury. 2018;49:2132-2141 pubmed publisher
Bergmann A, Schilling T, Hedenstierna G, Ahlgren K, Larsson A, Kretzschmar M, et al. Pulmonary effects of remote ischemic preconditioning in a porcine model of ventilation-induced lung injury. Respir Physiol Neurobiol. 2019;259:111-118 pubmed publisher
Kaiser M, Jacobson M, Andersen P, Bækbo P, Ceron J, Dahl J, et al. Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome. BMC Vet Res. 2018;14:83 pubmed publisher
Xu X, Chen S, Wang H, Tu Z, Wang S, Wang X, et al. Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide. Br J Nutr. 2018;119:1019-1028 pubmed publisher
Duburcq T, Durand A, Tournoys A, Gnemmi V, Gmyr V, Pattou F, et al. Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study. Ann Intensive Care. 2018;8:24 pubmed publisher
Kloubert V, Blaabjerg K, Dalgaard T, Poulsen H, Rink L, Wessels I. Influence of zinc supplementation on immune parameters in weaned pigs. J Trace Elem Med Biol. 2018;49:231-240 pubmed publisher
Goździk W, Zieliński S, Zielinska M, Ratajczak K, Skrzypczak P, Rodziewicz S, et al. Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping. Int J Immunopathol Pharmacol. 2018;32:394632017751486 pubmed publisher
Yan H, Ajuwon K. Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway. PLoS ONE. 2017;12:e0179586 pubmed publisher
Kern M, Günzel D, Aschenbach J, Tedin K, Bondzio A, Lodemann U. Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium. Mediators Inflamm. 2017;2017:2748192 pubmed publisher
Lukovic D, Zlabinger K, Gugerell A, Spannbauer A, Pavo N, Mandic L, et al. Inhibition of CD34+ cell migration by matrix metalloproteinase-2 during acute myocardial ischemia, counteracted by ischemic preconditioning. F1000Res. 2016;5:2739 pubmed publisher
Westover A, Johnston K, Buffington D, Humes H. An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome. J Diabetes Res. 2016;2016:3486727 pubmed
Bleilevens C, Grottke O, Groening S, Honickel M, Kopp R, Singh S, et al. Septic porcine blood does not further activate coagulation during in vitro membrane oxygenation. Eur J Cardiothorac Surg. 2017;51:449-456 pubmed publisher
Hartmann E, Ziebart A, Thomas R, Liu T, Schad A, Tews M, et al. Inhalation therapy with the synthetic TIP-like peptide AP318 attenuates pulmonary inflammation in a porcine sepsis model. BMC Pulm Med. 2015;15:7 pubmed publisher
Qu X, Pröll M, Neuhoff C, Zhang R, Cinar M, Hossain M, et al. Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide. PLoS ONE. 2015;10:e0121574 pubmed publisher
Clayton K, Douglas Vail M, Nur Ur Rahman A, Medcalf K, Xie I, Chew G, et al. Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression. J Virol. 2015;89:3723-36 pubmed publisher
Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, et al. Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs. Respir Res. 2014;15:101 pubmed publisher
Li X, Zhu Y, Zhang H, Yue Y, Cai Z, Lu Q, et al. Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea: intestinal microbiota and immune imbalances. PLoS ONE. 2012;7:e40666 pubmed publisher
Yeung J, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun Y, et al. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig. Mol Ther. 2012;20:1204-11 pubmed publisher
Barratt Due A, Thorgersen E, Lindstad J, Pharo A, Lissina O, Lambris J, et al. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans. J Immunol. 2011;187:4913-9 pubmed publisher
Martínez Olondris P, Rigol M, Soy D, Guerrero L, Agustí C, Quera M, et al. Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs. Crit Care Med. 2012;40:162-8 pubmed publisher
Hellerud B, Nielsen E, Thorgersen E, Lindstad J, Pharo A, Tønnessen T, et al. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis. Crit Care Med. 2010;38:1467-74 pubmed publisher
McILwain R, Timpa J, Kurundkar A, Holt D, Kelly D, Hartman Y, et al. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine. Lab Invest. 2010;90:128-39 pubmed publisher
Thorgersen E, Hellerud B, Nielsen E, Barratt Due A, Fure H, Lindstad J, et al. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J. 2010;24:712-22 pubmed publisher
Niemann J, Rosborough J, Youngquist S, Shah A, Lewis R, Phan Q, et al. Cardiac function and the proinflammatory cytokine response after recovery from cardiac arrest in swine. J Interferon Cytokine Res. 2009;29:749-58 pubmed publisher
Schuleri K, Feigenbaum G, Centola M, Weiss E, Zimmet J, Turney J, et al. Autologous mesenchymal stem cells produce reverse remodelling in chronic ischaemic cardiomyopathy. Eur Heart J. 2009;30:2722-32 pubmed publisher
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Thorgersen E, Pharo A, Haverson K, Axelsen A, Gaustad P, Kotwal G, et al. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood. Infect Immun. 2009;77:725-32 pubmed publisher
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Kruse R, Essen Gustavsson B, Fossum C, Jensen Waern M. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery. Acta Vet Scand. 2008;50:32 pubmed publisher
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Yano T, Itoh Y, Matsuo M, Kawashiri T, Egashira N, Oishi R. Involvement of both tumor necrosis factor-alpha-induced necrosis and p53-mediated caspase-dependent apoptosis in nephrotoxicity of cisplatin. Apoptosis. 2007;12:1901-9 pubmed
Luna C, Baquero S, Gando S, Patrón J, Morato J, Sibila O, et al. Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation. Chest. 2007;132:523-31 pubmed
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Zhu T, Sennlaub F, Beauchamp M, Fan L, Joyal J, Checchin D, et al. Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent. Arterioscler Thromb Vasc Biol. 2006;26:744-50 pubmed
Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide. J Nutr. 2005;135:239-44 pubmed
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Majetschak M, Krehmeier U, Bardenheuer M, Denz C, Quintel M, Voggenreiter G, et al. Extracellular ubiquitin inhibits the TNF-alpha response to endotoxin in peripheral blood mononuclear cells and regulates endotoxin hyporesponsiveness in critical illness. Blood. 2003;101:1882-90 pubmed
product information
master code :
PTA00
SKU :
PTA00
product name :
Porcine TNF-alpha Quantikine ELISA Kit
unit size :
1 Kit
description :
The Porcine TNF-alpha Quantikine ELISA Kit from R&D Systems is a Solid Phase Sandwich ELISA that quantifies porcine TNF-alpha in cell culture supernates (50 ul), serum (50 ul), edta plasma (50 ul), heparin plasma (50 ul).
target :
TNF-alpha
category :
ELISAs
species :
Porcine
specificity :
Natural and recombinant porcine TNF-alpha
gene symbol :
TNF
kit type :
Solid Phase Sandwich ELISA
assay length :
4.5 hours
elisaSampleTypes :
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
elisaSensitivity :
5 pg/mL
elisaRange :
23.40 - 1500 pg/mL
top caption :
Porcine TNF-alpha ELISA Cell Culture Supernate Standard Curve
applications :
ELISA
USD :
655 USD
product details :
The Quantikine Porcine TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine TNF-alpha in cell culture supernates, serum, and plasma samples. It contains E. coli -expressed recombinant porcine TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure recombinant porcine TNF-alpha. Results obtained using natural porcine TNF-alpha show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine TNF-alpha.
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Store the unopened product at 2 - 8 ░C. Do not use past expiration date.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.