Human/Mouse Cathepsin L Antibody | R&D Systems MAB9521-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human/Mouse Cathepsin L Antibody

catalog :

MAB9521-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

459 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

204101

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 12

Reference
Li Y, Jones J, M C Choi H, Sarkar C, KANE M, Koh E, et al. cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury. Cell Death Dis. 2019;10:531 pubmed publisher
Sarkar C, Jones J, Hegdekar N, Thayer J, Kumar A, Faden A, et al. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma. Autophagy. 2019;:1-20 pubmed publisher
Pavel M, Imarisio S, Menzies F, Jimenez Sanchez M, Siddiqi F, Wu X, et al. CCT complex restricts neuropathogenic protein aggregation via autophagy. Nat Commun. 2016;7:13821 pubmed publisher
Yang M, Liang C, Swaminathan K, Herrlinger S, Lai F, Shiekhattar R, et al. A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy. Sci Adv. 2016;2:e1601167 pubmed publisher
Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, et al. The PI3K regulatory subunits p55? and p50? regulate cell death in vivo. Cell Death Differ. 2014;21:1442-50 pubmed publisher
Campbell J, Botos L, Sargeant T, Davidenko N, Cameron R, Watson C. A 3-D in vitro co-culture model of mammary gland involution. Integr Biol (Camb). 2014;6:618-26 pubmed publisher
O Brien J, Martinson H, Durand Rougely C, Schedin P. Macrophages are crucial for epithelial cell death and adipocyte repopulation during mammary gland involution. Development. 2012;139:269-75 pubmed publisher
Kreuzaler P, Staniszewska A, Li W, Omidvar N, Kedjouar B, Turkson J, et al. Stat3 controls lysosomal-mediated cell death in vivo. Nat Cell Biol. 2011;13:303-9 pubmed publisher
Lopez Castejon G, Theaker J, Pelegrin P, Clifton A, Braddock M, Surprenant A. P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases. J Immunol. 2010;185:2611-9 pubmed publisher
Zeeuwen P, van Vlijmen Willems I, Cheng T, Rodijk Olthuis D, Hitomi K, Hara Nishimura I, et al. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J. 2010;24:3744-55 pubmed publisher
Parker B, Ciocca D, Bidwell B, Gago F, Fanelli M, George J, et al. Primary tumour expression of the cysteine cathepsin inhibitor Stefin A inhibits distant metastasis in breast cancer. J Pathol. 2008;214:337-46 pubmed
Cheng T, Hitomi K, van Vlijmen Willems I, de Jongh G, Yamamoto K, Nishi K, et al. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J Biol Chem. 2006;281:15893-9 pubmed

product information

brand :

R&D Systems

master code :

MAB9521

SKU :

MAB9521-100

product name :

Human/Mouse Cathepsin L Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human/Mouse Cathepsin L Antibody from R&D Systems is a rat monoclonal antibody to Cathepsin L. This antibody reacts with human,mouse. The Human/Mouse Cathepsin L Antibody has been validated for the following applications: Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunoprecipitation.

target :

Cathepsin L

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

204101

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Immunohistochemistry 5-25 ug/mL, Immunoprecipitation 25 ug/mL

host :

Rat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human Cathepsin L, Glu113-Val333, Accession # P07711

isotype :

IgG2a

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Mouse

specificity :

Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.

gene symbol :

CTSL1

top caption :

Detection of Recombinant Human and Mouse Cathepsin L antibody by Western Blot.

accessionNumbers :

P07711

applications :

Immunoprecipitation,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin

USD :

459 USD

alt names :

cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, CTSLEC 3.4.22.15, EC 3.4.22, FLJ31037, Major excreted protein, MEP

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage