Human TACE/ADAM17 Ectodomain Antibody | R&D Systems MAB9301-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human TACE/ADAM17 Ectodomain Antibody

catalog :

MAB9301-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

459 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

111633

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

R&D Systems product webpage

citations: 17

Reference
Le Trilling V, Maa xdf en F, Katschinski B, Hengel H, Trilling M. Deletion of the non-adjacent genes UL148 and UL148D impairs human cytomegalovirus-mediated TNF receptor 2 surface upregulation. Front Immunol. 2023;14:1170300 pubmed publisher
Rubina A, Patel M, Nightingale K, Potts M, Fielding C, Kollnberger S, et al. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways. Proc Natl Acad Sci U S A. 2023;120:e2303155120 pubmed publisher
Kwak M, Southard K, Kim W, Lin A, Kim N, Gopalappa R, et al. Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling. Nat Cell Biol. 2022;24:1739-1753 pubmed publisher
Somasundaram A, Cillo A, Lampenfeld C, Workman C, Kunning S, Oliveri L, et al. Systemic Immune Dysfunction in Cancer Patients Driven by IL6 Induction of LAG3 in Peripheral CD8+ T Cells. Cancer Immunol Res. 2022;10:885-899 pubmed publisher
Giese A, Babendreyer A, Krappen P, Gross A, Strnad P, Düsterhöft S, et al. Inflammatory activation of surface molecule shedding by upregulation of the pseudoprotease iRhom2 in colon epithelial cells. Sci Rep. 2021;11:24230 pubmed publisher
Pomeroy E, Hunzeker J, Kluesner M, Lahr W, Smeester B, Crosby M, et al. A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer Immunotherapy. Mol Ther. 2020;28:52-63 pubmed publisher
Raneros A, Puras A, Rodriguez R, Colado E, Bernal T, Anguita E, et al. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition. Oncotarget. 2017;8:31959-31976 pubmed publisher
Buchanan P, Boylan K, Walcheck B, Heinze R, Geller M, Argenta P, et al. Ectodomain shedding of the cell adhesion molecule Nectin-4 in ovarian cancer is mediated by ADAM10 and ADAM17. J Biol Chem. 2017;292:6339-6351 pubmed publisher
Dombernowsky S, SamsÃ¸e Petersen J, Petersen C, Instrell R, Hedegaard A, Thomas L, et al. The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17. Nat Commun. 2015;6:7518 pubmed publisher
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, et al. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol. 2015;195:736-48 pubmed publisher
Jing Y, Ni Z, Wu J, Higgins L, Markowski T, Kaufman D, et al. Identification of an ADAM17 cleavage region in human CD16 (FcÎ³RIII) and the engineering of a non-cleavable version of the receptor in NK cells. PLoS ONE. 2015;10:e0121788 pubmed publisher
Garbin U, Baggio E, Stranieri C, Pasini A, Manfro S, Mozzini C, et al. Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?. Cardiovasc Res. 2013;97:125-33 pubmed publisher
Breshears L, Schlievert P, Peterson M. A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1). J Biol Chem. 2012;287:32578-87 pubmed
Gutierrez Lopez M, Gilsanz A, Yanez Mo M, Ovalle S, Lafuente E, Dominguez C, et al. The sheddase activity of ADAM17/TACE is regulated by the tetraspanin CD9. Cell Mol Life Sci. 2011;68:3275-92 pubmed publisher
Villeneuve J, Block A, Le Bousse Kerdiles M, Lepreux S, Nurden P, Ripoche J, et al. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Exp Hematol. 2009;37:849-56 pubmed publisher
Hurst L, Bunning R, Couraud P, Romero I, Weksler B, Sharrack B, et al. Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. J Neuroimmunol. 2009;210:108-12 pubmed publisher
Koenen R, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, et al. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases. Blood. 2009;113:4799-809 pubmed publisher

product information

brand :

R&D Systems

master code :

MAB9301

SKU :

MAB9301-100

product name :

Human TACE/ADAM17 Ectodomain Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human TACE/ADAM17 Ectodomain Antibody from R&D Systems is a mouse monoclonal antibody to TACE/ADAM17. This antibody reacts with human. The Human TACE/ADAM17 Ectodomain Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Immunocytochemistry,Immunoprecipitation,CyTOF-ready,Knockout Validated.

target :

TACE/ADAM17

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

111633

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunoprecipitation 25 ug/mL, CyTOF-ready, Knockout Validated 0.25 ug/10^6 cells

host :

Mouse

immunogen :

Insect ovarian cell line T. ni -derived recombinant human TACE/ADAM17, Pro18-Asn671, Accession # P78536

isotype :

IgG1

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human

specificity :

Detects the ectodomain of human TACE/ADAM17 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.

gene symbol :

ADAM17

Antibody validation :

Knockout/Knockdown

top caption :

Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

accessionNumbers :

P78536

applications :

Immunoprecipitation,CyTOF-ready,Knockout Validated,Flow Cytometry,Western Blot,Immunocytochemistry

USD :

459 USD

alt names :

ADAM 17, ADAM metallopeptidase domain 17, ADAM metallopeptidase domain 18, ADAM17, ADAM18, CD156b, CD156b antigen, CSVP, disintegrin and metalloproteinase domain-containing protein 17, EC 3.4.24.86, MGC71942, Snake venom-like protease, TACEcSVP, TNF-alpha convertase, TNF-alpha converting enzyme, TNF-alpha-converting enzyme, tumor necrosis factor, alpha, converting enzyme

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage