Human CCL2/JE/MCP-1 Antibody | R&D Systems MAB679-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human CCL2/JE/MCP-1 Antibody

catalog :

MAB679-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

479 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

23007

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 50

Reference
Sinclair J, Vedelago C, Ryan F, Carney M, Redd M, Lynn M, et al. Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function. Nat Microbiol. 2024;9:3135-3147 pubmed publisher
Hong J, Son M, Sin J, Kim H, Chung D. Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation. Nutrients. 2023;15: pubmed publisher
Br xfc ser L, Teichmann E, Hinz B. Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes. Int J Mol Sci. 2023;24: pubmed publisher
Kahm Y, Jung U, Kim R. Regulation of Cancer Stem Cells and Epithelial-Mesenchymal Transition by CTNNAL1 in Lung Cancer and Glioblastoma. Biomedicines. 2023;11: pubmed publisher
Zhang Z, Xie Z, Lin J, Sun Z, Li Z, Yu W, et al. The m6A methyltransferase METTL16 negatively regulates MCP1 expression in mesenchymal stem cells during monocyte recruitment. JCI Insight. 2023;8: pubmed publisher
Parween F, Singh S, Zhang H, Kathuria N, Otaizo Carrasquero F, Shamsaddini A, et al. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells. bioRxiv. 2023;: pubmed publisher
Chin L, Yang J, Chousterman B, Jung S, Kim D, Kim D, et al. Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection. ACS Nano. 2023;17:3610-3619 pubmed publisher
Zhang J, Liu F, Yang Y, Yu N, Weng X, Yang Y, et al. Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer. Cell Death Dis. 2022;13:392 pubmed publisher
Luo L, Deng S, Tang W, Hu X, Yin F, Ge H, et al. Recruitment of IL-1β-producing intermediate monocytes enhanced by C5a contributes to the development of malignant pleural effusion. Thorac Cancer. 2022;13:811-823 pubmed publisher
Wang L, Lan J, Tang J, Luo N. MCP-1 targeting: Shutting off an engine for tumor development. Oncol Lett. 2022;23:26 pubmed publisher
Toledo N, Li H, Omange R, Dacoba T, Crecente Campo J, Schalk D, et al. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens. Front Immunol. 2020;11:1935 pubmed publisher
Abouelasrar Salama S, De Bondt M, Berghmans N, Gouwy M, de Oliveira V, Oliveira S, et al. Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case. Mediators Inflamm. 2020;2020:6087109 pubmed publisher
Wong S, Lenzini S, Cooper M, Mooney D, Shin J. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Sci Adv. 2020;6:eaaw0158 pubmed publisher
Abouelasrar Salama S, Gouwy M, De Zutter A, Pörtner N, Vanbrabant L, Berghmans N, et al. Induction of Chemokines by Hepatitis C Virus Proteins: Synergy of the Core Protein with Interleukin-1β and Interferon-γ in Liver Bystander Cells. J Interferon Cytokine Res. 2020;40:195-206 pubmed publisher
Ryan D, Koziol J, ElShamy W. Targeting AXL and RAGE to prevent geminin overexpression-induced triple-negative breast cancer metastasis. Sci Rep. 2019;9:19150 pubmed publisher
Sun C, Li X, Guo E, Li N, Zhou B, Lu H, et al. MCP-1/CCR-2 axis in adipocytes and cancer cell respectively facilitates ovarian cancer peritoneal metastasis. Oncogene. 2019;: pubmed publisher
Salah S, Meisenheimer J, Rao R, Peda J, Wallace D, Foster D, et al. MCP-1 promotes detrimental cardiac physiology, pulmonary edema, and death in the cpk model of polycystic kidney disease. Am J Physiol Renal Physiol. 2019;317:F343-F360 pubmed publisher
Kim K, Kuang S, Song Q, Gavin T, Roseguini B. Impact of heat therapy on recovery after eccentric exercise in humans. J Appl Physiol (1985). 2019;126:965-976 pubmed publisher
Kim H, Lee S, Lee S, Oh B, Kim J, Kim J, et al. A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells. Stem Cells. 2019;37:77-88 pubmed publisher
Wang T, Zhan Q, Peng X, Qiu Z, Zhao T. CCL2 influences the sensitivity of lung cancer A549 cells to docetaxel. Oncol Lett. 2018;16:1267-1274 pubmed publisher
Gril B, Paranjape A, Woditschka S, Hua E, Dolan E, Hanson J, et al. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases. Nat Commun. 2018;9:2705 pubmed publisher
Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, et al. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α. Mol Med Rep. 2018;18:1987-1994 pubmed publisher
Bian Z, Field M, Elner S, Kahlenberg J, Elner V. Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1β and MCP-1 in cultured human retinal pigment epithelial cells. Exp Eye Res. 2018;170:29-39 pubmed publisher
van Sleen Y, Wang Q, van der Geest K, Westra J, Abdulahad W, Heeringa P, et al. Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis. Sci Rep. 2017;7:6553 pubmed publisher
Henry C, Martin S. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation. Mol Cell. 2017;65:715-729.e5 pubmed publisher
Arakaki R, Yamasaki T, Kanno T, Shibasaki N, Sakamoto H, Utsunomiya N, et al. CCL2 as a potential therapeutic target for clear cell renal cell carcinoma. Cancer Med. 2016;5:2920-2933 pubmed publisher
Williams A, José R, Mercer P, Brealey D, Parekh D, Thickett D, et al. Evidence for chemokine synergy during neutrophil migration in ARDS. Thorax. 2017;72:66-73 pubmed publisher
Connelly K, Kandane Rathnayake R, Hoi A, Nikpour M, Morand E. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus. Sci Rep. 2016;6:29909 pubmed publisher
Li Y, Zheng Y, Li T, Wang Q, Qian J, Lu Y, et al. Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation, and polarization in multiple myeloma. Oncotarget. 2015;6:24218-29 pubmed
Katanov C, Lerrer S, Liubomirski Y, Leider Trejo L, Meshel T, Bar J, et al. Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-? and the NF-?B pathway. Stem Cell Res Ther. 2015;6:87 pubmed publisher
Sahler J, Woeller C, Phipps R. Microparticles engineered to highly express peroxisome proliferator-activated receptor-Î³ decreased inflammatory mediator production and increased adhesion of recipient monocytes. PLoS ONE. 2014;9:e113189 pubmed publisher
Hol J, Otterdal K, Breland U, Stang E, Pedersen T, Hagelsteen K, et al. Statins affect the presentation of endothelial chemokines by targeting to multivesicular bodies. PLoS ONE. 2012;7:e40673 pubmed publisher
Said N, Sanchez Carbayo M, Smith S, Theodorescu D. RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. 2012;122:1503-18 pubmed publisher
Kanno T, Kamba T, Yamasaki T, Shibasaki N, Saito R, Terada N, et al. JunB promotes cell invasion and angiogenesis in VHL-defective renal cell carcinoma. Oncogene. 2012;31:3098-110 pubmed publisher
Xi X, McMillan D, Lehmann G, Sime P, Libby R, Huxlin K, et al. Ocular fibroblast diversity: implications for inflammation and ocular wound healing. Invest Ophthalmol Vis Sci. 2011;52:4859-65 pubmed publisher
Soria G, Ofri Shahak M, Haas I, Yaal Hahoshen N, Leider Trejo L, Leibovich Rivkin T, et al. Inflammatory mediators in breast cancer: coordinated expression of TNF? & IL-1? with CCL2 & CCL5 and effects on epithelial-to-mesenchymal transition. BMC Cancer. 2011;11:130 pubmed publisher
Schipper H, de Jager W, van Dijk M, Meerding J, Zelissen P, Adan R, et al. A multiplex immunoassay for human adipokine profiling. Clin Chem. 2010;56:1320-8 pubmed publisher
Wu W, Mehta H, Chakrabarty K, Booth J, Duggan E, Patel K, et al. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J Immunol. 2009;183:5799-806 pubmed publisher
Hu H, Kwun J, Aizenstein B, Knechtle S. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation. 2009;87:1814-20 pubmed publisher
Struyf S, Proost P, Vandercappellen J, Dempe S, Noyens B, Nelissen S, et al. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects. Eur J Immunol. 2009;39:843-57 pubmed publisher
Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
Pfleger C, Schloot N, ter Veld F. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J Immunol Methods. 2008;329:214-8 pubmed
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
Zhao Z, McCloud B, Fleming R, Klempner M. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro. Biochem Biophys Res Commun. 2007;358:528-33 pubmed
Otterdal K, Smith C, Oie E, Pedersen T, Yndestad A, Stang E, et al. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood. 2006;108:928-35 pubmed
Saeed R, Varma S, Peng Nemeroff T, Sherry B, Balakhaneh D, Huston J, et al. Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation. J Exp Med. 2005;201:1113-23 pubmed
Mozaffarian D, Rimm E, King I, Lawler R, McDonald G, Levy W. trans fatty acids and systemic inflammation in heart failure. Am J Clin Nutr. 2004;80:1521-5 pubmed
Eugenin E, Dyer G, Calderon T, Berman J. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS. Glia. 2005;49:501-10 pubmed
Carter A, Aggarwal M, Kopia G, Tio F, Tsao P, Kolata R, et al. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model. Cardiovasc Res. 2004;63:617-24 pubmed
Øynebråten I, Bakke O, Brandtzaeg P, Johansen F, Haraldsen G. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Blood. 2004;104:314-20 pubmed

product information

brand :

R&D Systems

master code :

MAB679

SKU :

MAB679-100

product name :

Human CCL2/JE/MCP-1 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human CCL2/JE/MCP-1 Antibody from R&D Systems is a mouse monoclonal antibody to CCL2/JE/MCP-1. This antibody reacts with human,mouse,porcine. The Human CCL2/JE/MCP-1 Antibody has been validated for the following applications: Bioassay,Neutralization,Western Blot,Immunocytochemistry,Antibody Array Development,ELISA Development,ELISA Development (Capture),Luminex Development,ELISA,Functional Assay,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),Dual RNAscope ISH-IHC Compatible.

target :

CCL2/JE/MCP-1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

23007

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Immunohistochemistry 5-15 ug/mL, Neutralization 0.5-2.0 ug/mL, Dual RNAscope ISH-IHC 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

host :

Mouse

immunogen :

E. coli -derived recombinant human CCL2/JE/MCP-1

isotype :

IgG2b

purity :

Protein A or G purified from ascites

species :

Human,Mouse,Porcine

specificity :

Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) CCL2, 3, 4, rhCCL3, 4, 5, 7, or 8.

gene symbol :

CCL2

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

CCL2/JE/MCP-1 antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P).

applications :

Immunohistochemistry,ELISA Capture (Matched Antibody Pair),Dual RNAscope ISH-IHC Compatible,Antibody Array Development,Bioassay,ELISA Development,ELISA Development (Capture),Luminex Development,ELISA,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,Functional Assay,ELISA Capture

USD :

479 USD

alt names :

C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, Mcp1, MCP-1, SCYA2, SMC-CF

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage