Human TNF-alpha Antibody | R&D Systems MAB610 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human TNF-alpha Antibody

catalog :

MAB610

quantity :

100 ug (also 25 ug, 500 ug)

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

28401

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, neutralization, blocking or activating experiments

citations: 41

Published Application/Species/Sample/Dilution	Reference
ELISA; human; loading ...; tbl s7	Bouhaddou M, Memon D, Meyer B, White K, Rezelj V, Correa Marrero M, et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020;182:685-712.e19 pubmed publisher
blocking or activating experiments; human; loading ...; fig s2	Tanzer M, Khan N, Rickard J, Etemadi N, Lalaoui N, Spall S, et al. Combination of IAP antagonist and IFNÎ³ activates novel caspase-10- and RIPK1-dependent cell death pathways. Cell Death Differ. 2017;24:481-491 pubmed publisher
	Seo J, Seong D, Nam Y, Hwang C, Lee S, Lee C, et al. Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex. Cell Death Differ. 2020;: pubmed publisher
	Stumpf A, Brandstetter T, Hübner J, Rühe J. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum. PLoS ONE. 2019;14:e0225525 pubmed publisher
	Hoste E, Maueröder C, Van Hove L, Catrysse L, Vikkula H, Sze M, et al. Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation. Cell Rep. 2019;29:2689-2701.e4 pubmed publisher
	Wang Y, Liu J, Chen X, Sun H, Peng S, Kuang Y, et al. Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-кB and IL-1β signal pathways. J Cell Mol Med. 2019;23:476-486 pubmed publisher
	Sun W, Meednu N, Rosenberg A, Rangel Moreno J, Wang V, Glanzman J, et al. B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation. Nat Commun. 2018;9:5127 pubmed publisher
	Urra S, Fischer M, Martinez J, Veliz L, Orellana P, Solar A, et al. Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis?. Oncotarget. 2018;9:2445-2467 pubmed publisher
	Ouhara K, Munenaga S, Kajiya M, Takeda K, Matsuda S, Sato Y, et al. The induced RNA-binding protein, HuR, targets 3'-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis. Clin Exp Immunol. 2018;192:325-336 pubmed publisher
	Regan T, Gill A, Clohisey S, Barnett M, Pariante C, Harrison N, et al. Effects of anti-inflammatory drugs on the expression of tryptophan-metabolism genes by human macrophages. J Leukoc Biol. 2018;103:681-692 pubmed publisher
	Henry C, Martin S. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation. Mol Cell. 2017;65:715-729.e5 pubmed publisher
	Jiang H, Zhang X, Lin H. Lysine fatty acylation promotes lysosomal targeting of TNF-?. Sci Rep. 2016;6:24371 pubmed publisher
	Matt P, Lindqvist U, Kleinau S. Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcÎ³R Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment. PLoS ONE. 2015;10:e0137474 pubmed publisher
	Zuo J, Shan Z, Zhou L, Yu J, Liu X, Gao Y. Increased CD160 expression on circulating natural killer cells in atherogenesis. J Transl Med. 2015;13:188 pubmed publisher
	Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, et al. Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma. PLoS ONE. 2014;9:e114345 pubmed publisher
	Todd J, Simpson P, Estis J, Torres V, Wub A. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine. 2013;64:660-5 pubmed publisher
	Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, et al. The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells. Clin Cancer Res. 2013;19:1784-94 pubmed publisher
	Kulbe H, Chakravarty P, Leinster D, Charles K, Kwong J, Thompson R, et al. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res. 2012;72:66-75 pubmed publisher
	Liu F, Chen H, Huang X, Chung A, Zhou L, Fu P, et al. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia. 2011;54:2713-23 pubmed publisher
	Zepp J, Nold Petry C, Dinarello C, Nold M. Protection from RNA and DNA viruses by IL-32. J Immunol. 2011;186:4110-8 pubmed publisher
	Wu W, Mehta H, Chakrabarty K, Booth J, Duggan E, Patel K, et al. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J Immunol. 2009;183:5799-806 pubmed publisher
	Hu H, Kwun J, Aizenstein B, Knechtle S. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation. 2009;87:1814-20 pubmed publisher
	Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
	Biancotto A, Iglehart S, Lisco A, Vanpouille C, Grivel J, Lurain N, et al. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res Hum Retroviruses. 2008;24:453-62 pubmed publisher
	Kim H, Kim N, Gim M, Lee J, Lee S, Ko M, et al. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J Immunol. 2008;180:2553-61 pubmed
	Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
	John T, Muller R, Oberholzer A, Zreiqat H, Kohl B, Ertel W, et al. Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro. Cytokine. 2007;40:226-34 pubmed
	Harrison M, Obermueller E, Maisey N, Hoare S, Edmonds K, Li N, et al. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. J Clin Oncol. 2007;25:4542-9 pubmed
	Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
	Grivel J, Elliott J, Lisco A, Biancotto A, Condack C, Shattock R, et al. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS. 2007;21:1263-72 pubmed
	Colucci S, Brunetti G, Cantatore F, Oranger A, Mori G, Quarta L, et al. Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. J Pathol. 2007;212:47-55 pubmed
	Biancotto A, Grivel J, Iglehart S, Vanpouille C, Lisco A, Sieg S, et al. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood. 2007;109:4272-9 pubmed
	Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, et al. Selective regulation of IL-10 signaling and function by zymosan. J Immunol. 2006;176:4785-92 pubmed
	Wunder D, Mueller M, Birkhäuser M, Bersinger N. Increased ENA-78 in the follicular fluid of patients with endometriosis. Acta Obstet Gynecol Scand. 2006;85:336-42 pubmed
	Hangai M, He S, Hoffmann S, Lim J, Ryan S, Hinton D. Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells. J Neuroimmunol. 2006;171:45-56 pubmed
	Mozaffarian D, Rimm E, King I, Lawler R, McDonald G, Levy W. trans fatty acids and systemic inflammation in heart failure. Am J Clin Nutr. 2004;80:1521-5 pubmed
	Phillips T. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis. 2004;25:1652-9 pubmed
	Sharif M, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv L. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. J Immunol. 2004;172:6476-81 pubmed
	Buch M, Conaghan P, Quinn M, Bingham S, Veale D, Emery P. True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?. Ann Rheum Dis. 2004;63:1344-6 pubmed
	Copeland S, Siddiqui J, Remick D. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J Immunol Methods. 2004;284:99-106 pubmed
	Herrero C, Hu X, Li W, Samuels S, Sharif M, Kotenko S, et al. Reprogramming of IL-10 activity and signaling by IFN-gamma. J Immunol. 2003;171:5034-41 pubmed

product information

brand :

R&D Systems

master code :

MAB610

SKU :

MAB610-100

product name :

Human TNF-alpha Antibody

description :

The Human TNF-alpha Antibody from R&D Systems is a mouse monoclonal antibody to TNF-alpha. This antibody reacts with human. The Human TNF-alpha Antibody has been validated for the following applications: Western Blot, Neutralization, Immunocytochemistry, ELISA Capture (Matched Antibody Pair).

target :

TNF-alpha

category :

Primary Antibodies

unit size :

100 ug (also 25 ug, 500 ug)

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

clonality :

Monoclonal

clone :

28401

conjugate :

Unconjugated

host :

Mouse

immunogen :

E. coli -derived recombinant human TNF-alpha , Gly57-Leu233 (predicted), Accession # P01375

isotype :

IgG1

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human

specificity :

Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF-beta , recombinant mouse TNF-alpha , recombinant rat TNF-alpha , and recombinant porcine TNF-alpha is observed.

gene symbol :

TNF

endotoxin note :

<0.10 EU per 1 µg of the antibody by the LAL method.

catalog number base :

MAB610

accessionNumbers :

P01375

applications :

Western Blot, Neutralization, Immunocytochemistry, ELISA Capture (Matched Antibody Pair)

2020 USD :

339

2021 USD :

369 USD

alt names :

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.