product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human TNF-alpha Antibody
catalog :
MAB610-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
479 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
28401
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 70
Reference
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Tong M, Potter J, Mor G, Abrahams V. Lipopolysaccharide-Stimulated Human Fetal Membranes Induce Neutrophil Activation and Release of Vital Neutrophil Extracellular Traps. J Immunol. 2019;: pubmed publisher
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Pehrson R, Hegelund Myrbäck T, Cunoosamy D, Asimus S, Jansson P, Patel N, et al. AZD7624, an Inhaled p38 Inhibitor, Demonstrates Local Lung Inhibition of LPS-Induced TNFα with Minimal Systemic Exposure. J Pharmacol Exp Ther. 2018;365:567-572 pubmed publisher
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Castellanos J, Neissa J, Camacho S. Dengue virus induces apoptosis in SH-SY5Y human neuroblastoma cells. Biomedica. 2016;36:156-8 pubmed publisher
Jiang H, Zhang X, Lin H. Lysine fatty acylation promotes lysosomal targeting of TNF-?. Sci Rep. 2016;6:24371 pubmed publisher
Matt P, Lindqvist U, Kleinau S. Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcγR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment. PLoS ONE. 2015;10:e0137474 pubmed publisher
Valentina M, Jan F, Peder N, Bo Z, Hongjie D, Pernille K. Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips. BMC Biotechnol. 2015;15:73 pubmed publisher
Zuo J, Shan Z, Zhou L, Yu J, Liu X, Gao Y. Increased CD160 expression on circulating natural killer cells in atherogenesis. J Transl Med. 2015;13:188 pubmed publisher
Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, et al. Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma. PLoS ONE. 2014;9:e114345 pubmed publisher
England E, Newton P, Neal F, Kitching L, Colley C, Rossant C. Application of the mirrorball high-sensitivity cytometer to multiplexed assays for antibody drug discovery. J Biomol Screen. 2015;20:536-44 pubmed publisher
Melin E, Sohrabian A, R xf6 nnelid J, Borg K. Normal serum levels of immune complexes in postpolio patients. Results Immunol. 2014;4:54-7 pubmed publisher
Bornot A, Blackett C, Engkvist O, Murray C, Bendtsen C. The Role of Historical Bioactivity Data in the Deconvolution of Phenotypic Screens. J Biomol Screen. 2014;19:696-706 pubmed publisher
Todd J, Simpson P, Estis J, Torres V, Wub A. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine. 2013;64:660-5 pubmed publisher
Chen X, Chang J, Deng Q, Xu J, Nguyen T, Martens L, et al. Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells. J Neurosci. 2013;33:9202-13 pubmed publisher
Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, et al. The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells. Clin Cancer Res. 2013;19:1784-94 pubmed publisher
Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, et al. Simultaneous immunoassay analysis of plasma IL-6 and TNF-? on a microchip. PLoS ONE. 2013;8:e53620 pubmed publisher
Kulbe H, Chakravarty P, Leinster D, Charles K, Kwong J, Thompson R, et al. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res. 2012;72:66-75 pubmed publisher
Liu F, Chen H, Huang X, Chung A, Zhou L, Fu P, et al. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia. 2011;54:2713-23 pubmed publisher
Zepp J, Nold Petry C, Dinarello C, Nold M. Protection from RNA and DNA viruses by IL-32. J Immunol. 2011;186:4110-8 pubmed publisher
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Wu W, Mehta H, Chakrabarty K, Booth J, Duggan E, Patel K, et al. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J Immunol. 2009;183:5799-806 pubmed publisher
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Kim H, Kim N, Gim M, Lee J, Lee S, Ko M, et al. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J Immunol. 2008;180:2553-61 pubmed
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product information
master code :
MAB610
SKU :
MAB610-100
product name :
Human TNF-alpha Antibody
unit size :
100 ug (also 25 ug, 500 ug)
description :
The Human TNF-alpha Antibody from R&D Systems is a mouse monoclonal antibody to TNF-alpha. This antibody reacts with human,mouse,n/a. The Human TNF-alpha Antibody has been validated for the following applications: ELISA Capture,Assay Development,Affinity Chromatography,Antibody Array Development,Array Development,Bioassay,Electrochemiluminescent Assay,ELISA Development,ELISA Development (Capture),ELISA Microarray Development,Luminex Development,Immunoprecipitation,Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Block/Neutralize,ELISA Capture (Matched Antibody Pair).
target :
TNF-alpha
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
28401
conjugate :
Unconjugated
host :
Mouse
immunogen :
E. coli -derived recombinant human TNF-alpha , Gly57-Leu233 (predicted), Accession # P01375
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,N/A
specificity :
Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF┐▀, recombinant mouse TNF-alpha , recombinant rat TNF-alpha , and recombinant porcine TNF-alpha is observed.
gene symbol :
TNF
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Recombinant Human TNF-a antibody by Western Blot.
accessionNumbers :
P01375
applications :
ELISA Capture,Assay Development,Affinity Chromatography,Antibody Array Development,Array Development,Bioassay,Electrochemiluminescent Assay,ELISA Development,ELISA Development (Capture),ELISA Microarray Development,Luminex Development,Immunoprecipitation,Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Block/Neutralize,ELISA Capture (Matched Antibody Pair)
USD :
479 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.