product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human TNF-alpha Antibody
catalog :
MAB610-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
479 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
28401
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 70
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Kim H, Kim N, Gim M, Lee J, Lee S, Ko M, et al. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J Immunol. 2008;180:2553-61 pubmed
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Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
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John T, Muller R, Oberholzer A, Zreiqat H, Kohl B, Ertel W, et al. Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro. Cytokine. 2007;40:226-34 pubmed
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Harrison M, Obermueller E, Maisey N, Hoare S, Edmonds K, Li N, et al. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. J Clin Oncol. 2007;25:4542-9 pubmed
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Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
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Grivel J, Elliott J, Lisco A, Biancotto A, Condack C, Shattock R, et al. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS. 2007;21:1263-72 pubmed
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Colucci S, Brunetti G, Cantatore F, Oranger A, Mori G, Quarta L, et al. Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. J Pathol. 2007;212:47-55 pubmed
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Biancotto A, Grivel J, Iglehart S, Vanpouille C, Lisco A, Sieg S, et al. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood. 2007;109:4272-9 pubmed
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Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, et al. Selective regulation of IL-10 signaling and function by zymosan. J Immunol. 2006;176:4785-92 pubmed
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Wunder D, Mueller M, Birkhäuser M, Bersinger N. Increased ENA-78 in the follicular fluid of patients with endometriosis. Acta Obstet Gynecol Scand. 2006;85:336-42 pubmed
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Hangai M, He S, Hoffmann S, Lim J, Ryan S, Hinton D. Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells. J Neuroimmunol. 2006;171:45-56 pubmed
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Mozaffarian D, Rimm E, King I, Lawler R, McDonald G, Levy W. trans fatty acids and systemic inflammation in heart failure. Am J Clin Nutr. 2004;80:1521-5 pubmed
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Phillips T. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis. 2004;25:1652-9 pubmed
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Sharif M, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv L. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. J Immunol. 2004;172:6476-81 pubmed
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Buch M, Conaghan P, Quinn M, Bingham S, Veale D, Emery P. True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?. Ann Rheum Dis. 2004;63:1344-6 pubmed
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Copeland S, Siddiqui J, Remick D. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J Immunol Methods. 2004;284:99-106 pubmed
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Herrero C, Hu X, Li W, Samuels S, Sharif M, Kotenko S, et al. Reprogramming of IL-10 activity and signaling by IFN-gamma. J Immunol. 2003;171:5034-41 pubmed
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product information
master code :
MAB610
SKU :
MAB610-100
product name :
Human TNF-alpha Antibody
unit size :
100 ug (also 25 ug, 500 ug)
description :
The Human TNF-alpha Antibody from R&D Systems is a mouse monoclonal antibody to TNF-alpha. This antibody reacts with human,mouse,n/a. The Human TNF-alpha Antibody has been validated for the following applications: ELISA Capture,Assay Development,Affinity Chromatography,Antibody Array Development,Array Development,Bioassay,Electrochemiluminescent Assay,ELISA Development,ELISA Development (Capture),ELISA Microarray Development,Luminex Development,Immunoprecipitation,Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Block/Neutralize,ELISA Capture (Matched Antibody Pair).
target :
TNF-alpha
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
28401
conjugate :
Unconjugated
host :
Mouse
immunogen :
E. coli -derived recombinant human TNF-alpha , Gly57-Leu233 (predicted), Accession # P01375
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,N/A
specificity :
Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF┐▀, recombinant mouse TNF-alpha , recombinant rat TNF-alpha , and recombinant porcine TNF-alpha is observed.
gene symbol :
TNF
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Recombinant Human TNF-a antibody by Western Blot.
accessionNumbers :
P01375
applications :
ELISA Capture,Assay Development,Affinity Chromatography,Antibody Array Development,Array Development,Bioassay,Electrochemiluminescent Assay,ELISA Development,ELISA Development (Capture),ELISA Microarray Development,Luminex Development,Immunoprecipitation,Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Block/Neutralize,ELISA Capture (Matched Antibody Pair)
USD :
479 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information

R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.
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