product summary
Loading...
company name :
R&D Systems
product type :
antibody
product name :
Mouse TNF-alpha Antibody
catalog :
MAB4101
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MP6-XT22
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, blocking or activating experiments
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; mouse; loading ...; fig 7c
Shi L, Wang J, Ding N, Zhang Y, Zhu Y, Dong S, et al. Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy. Nat Commun. 2019;10:5421 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig s2
Kassem A, Lindholm C, Lerner U. Toll-Like Receptor 2 Stimulation of Osteoblasts Mediates Staphylococcus Aureus Induced Bone Resorption and Osteoclastogenesis through Enhanced RANKL. PLoS ONE. 2016;11:e0156708 pubmed publisher
  • blocking or activating experiments; mouse
Chucair Elliott A, Conrady C, Zheng M, Kroll C, Lane T, Carr D. Microglia-induced IL-6 protects against neuronal loss following HSV-1 infection of neural progenitor cells. Glia. 2014;62:1418-34 pubmed publisher
  • blocking or activating experiments; mouse
Notomi S, Hisatomi T, Kanemaru T, Takeda A, Ikeda Y, Enaida H, et al. Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. Am J Pathol. 2011;179:2798-809 pubmed publisher
  • blocking or activating experiments; mouse
Cappello P, Caorsi C, Bosticardo M, De Angelis S, Novelli F, Forni G, et al. CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity. J Leukoc Biol. 2004;75:135-42 pubmed
Yee Mon K, Kim S, Dai Z, West J, Zhu H, Jain R, et al. Functionalized nanowires for miRNA-mediated therapeutic programming of naïve T cells. Nat Nanotechnol. 2024;19:1190-1202 pubmed publisher
Oh J, Riek A, Bauerle K, Dusso A, McNerney K, Barve R, et al. Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes. Nat Commun. 2023;14:3278 pubmed publisher
Zhang H, Chen G, Feng X, Song H, Meng L, Fu Y, et al. Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression. EMBO Mol Med. 2023;15:e15924 pubmed publisher
Ferreira A, Sacramento C, Pereira Dutra F, Fintelman Rodrigues N, Silva P, Mattos M, et al. Severe influenza infection is associated with inflammatory programmed cell death in infected macrophages. Front Cell Infect Microbiol. 2023;13:1067285 pubmed publisher
Wu J, Luo F, Xie Y, Xiong H, Gao Y, Liu G, et al. EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway. Front Immunol. 2022;13:943174 pubmed publisher
Li Y, Zhu Y, Feng S, Ishida Y, Chiu T, Saito T, et al. Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3. Cell Rep. 2022;38:110284 pubmed publisher
Sun L, Kees T, Almeida A, Liu B, He X, Ng D, et al. Activating a collaborative innate-adaptive immune response to control metastasis. Cancer Cell. 2021;39:1361-1374.e9 pubmed publisher
Kothari V, Tang J, He Y, Kramer F, Kanter J, Bornfeldt K. ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages. Arterioscler Thromb Vasc Biol. 2021;41:1854-1873 pubmed publisher
Cai J, Hu D, Sakya J, Sun T, Wang D, Wang L, et al. ABIN-1 is a key regulator in RIPK1-dependent apoptosis (RDA) and necroptosis, and ABIN-1 deficiency potentiates necroptosis-based cancer therapy in colorectal cancer. Cell Death Dis. 2021;12:140 pubmed publisher
Liu Z, Nailwal H, Rector J, Rahman M, Sam R, McFadden G, et al. A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation. Immunity. 2021;54:247-258.e7 pubmed publisher
Kwok C, Learoyd A, Canet Pons J, Trang T, Fitzgerald M. Spinal interleukin-6 contributes to central sensitisation and persistent pain hypersensitivity in a model of juvenile idiopathic arthritis. Brain Behav Immun. 2020;90:145-154 pubmed publisher
Morris E, Suchacki K, Hocking J, Cartwright R, Sowman A, Gámez B, et al. Myeloma Cells Down-Regulate Adiponectin in Bone Marrow Adipocytes Via TNF-Alpha. J Bone Miner Res. 2020;35:942-955 pubmed publisher
Singh A, Jones O, Mockett B, Ohline S, Abraham W. Tumor Necrosis Factor-α-Mediated Metaplastic Inhibition of LTP Is Constitutively Engaged in an Alzheimer's Disease Model. J Neurosci. 2019;39:9083-9097 pubmed publisher
Song G, Rahman M, Jha M, Gupta D, Park S, Kim J, et al. A Bcr-Abl Inhibitor GNF-2 Attenuates Inflammatory Activation of Glia and Chronic Pain. Front Pharmacol. 2019;10:543 pubmed publisher
Shahriary G, Kataria H, Karimi Abdolrezaee S. Neuregulin-1 Fosters Supportive Interactions between Microglia and Neural Stem/Progenitor Cells. Stem Cells Int. 2019;2019:8397158 pubmed publisher
Handa P, Thomas S, Morgan Stevenson V, Maliken B, Gochanour E, Boukhar S, et al. Iron alters macrophage polarization status and leads to steatohepatitis and fibrogenesis. J Leukoc Biol. 2019;105:1015-1026 pubmed publisher
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, et al. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC. Oncogene. 2019;38:4366-4383 pubmed publisher
Chhana A, Pool B, Callon K, Tay M, Musson D, Naot D, et al. Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state. Arthritis Res Ther. 2018;20:208 pubmed publisher
Quiroga D, Muñoz M, Gil C, Pffeifer M, Toblli J, Steckelings U, et al. Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice. Physiol Rep. 2018;6:e13824 pubmed publisher
Cook A, Lee M, Saleh R, Khiew H, Christensen A, Achuthan A, et al. TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17. JCI Insight. 2018;3: pubmed publisher
Chen Y, Zhao Q, Sun Y, Jin Y, Zhang J, Wu J. Melatonin induces anti-inflammatory effects via endoplasmic reticulum stress in RAW264.7 macrophages. Mol Med Rep. 2018;17:6122-6129 pubmed publisher
Burg A, Das S, Padgett L, Koenig Z, Tse H. Superoxide Production by NADPH Oxidase Intensifies Macrophage Antiviral Responses during Diabetogenic Coxsackievirus Infection. J Immunol. 2018;200:61-70 pubmed publisher
Tavares R, Pathak S. Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways. Front Cell Infect Microbiol. 2017;7:58 pubmed publisher
Schock S, Chandra N, Sun Y, Irie T, Kitagawa Y, Gotoh B, et al. Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway. Cell Death Differ. 2017;24:615-625 pubmed publisher
Prasad S, Starck S, Shastri N. Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli. J Immunol. 2016;197:2981-2991 pubmed
Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, et al. Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice. Nat Med. 2015;21:1085-90 pubmed publisher
Berod L, Stüve P, Swallow M, Arnold Schrauf C, Kruse F, Gentilini M, et al. MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection. Eur J Immunol. 2014;44:1399-409 pubmed publisher
Ruiz E, Oeztuerk Winder F, Ventura J. A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Nat Commun. 2014;5:3175 pubmed publisher
Bhargava R, Altmann C, Andres Hernando A, Webb R, Okamura K, Yang Y, et al. Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-? antibodies. PLoS ONE. 2013;8:e79037 pubmed publisher
Pathak S, Tavares R, de Klerk N, Spetz A, Jonsson A. Helicobacter pylori protein JHP0290 binds to multiple cell types and induces macrophage apoptosis via tumor necrosis factor (TNF)-dependent and independent pathways. PLoS ONE. 2013;8:e77872 pubmed publisher
Gardner T, Chen Q, Jin Y, Ajuebor M. Toll-like receptor 3 ligand dampens liver inflammation by stimulating Valpha 14 invariant natural killer T cells to negatively regulate gammadeltaT cells. Am J Pathol. 2010;176:1779-89 pubmed publisher
Ismail N, Crossley E, Stevenson H, Walker D. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis. Infect Immun. 2007;75:4608-20 pubmed
product information
master code :
MAB4101
SKU :
MAB4101
product name :
Mouse TNF-alpha Antibody
unit size :
100 ug (also 25 ug)
description :
The Mouse TNF-alpha Antibody from R&D Systems is a rat monoclonal antibody to TNF-alpha. This antibody reacts with human,mouse,transgenic mouse. The Mouse TNF-alpha Antibody has been validated for the following applications: Neutralization,In vivo assay,Flow Cytometry,Bioassay,Western Blot,Cell Culture,ELISA Capture,Immunohistochemistry,Immunoprecipitation,Intracellular Staining by Flow Cytometry.
target :
TNF-alpha
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
MP6-XT22
concentration :
LYOPH
conjugate :
Unconjugated
host :
Rat
immunogen :
mouse TNF-alpha
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,Transgenic Mouse
specificity :
Detects mouse TNF-alpha .
gene symbol :
TNF
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
applications :
In vivo assay,Flow Cytometry,Bioassay,Western Blot,Neutralization,Cell Culture,ELISA Capture,Immunohistochemistry,Immunoprecipitation,Intracellular Staining by Flow Cytometry
USD :
489 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.