product summary
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company name :
R&D Systems
product type :
antibody
product name :
IL-6 Antibody
catalog :
MAB406-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
453 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MP5-20F3
reactivity :
mouse
application :
western blot, ELISA, neutralization, blocking or activating experiments
more info or order :
citations: 66
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1f
Cao Y, Huang W, Wu F, Shang J, Ping F, Wang W, et al. ZFP36 protects lungs from intestinal I/R-induced injury and fibrosis through the CREBBP/p53/p21/Bax pathway. Cell Death Dis. 2021;12:685 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 4g
  • ELISA; mouse; loading ...; fig 4c
Kumar A, Sundaram K, Mu J, Dryden G, Sriwastva M, Lei C, et al. High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance. Nat Commun. 2021;12:213 pubmed publisher
Guo R, Jiang M, Wang G, Li B, Jia X, Ai Y, et al. IL6 supports long-term expansion of hepatocytes in vitro. Nat Commun. 2022;13:7345 pubmed publisher
Paldor M, Levkovitch Siany O, Eidelshtein D, Adar R, Enk C, Marmary Y, et al. Single-cell transcriptomics reveals a senescence-associated IL-6/CCR6 axis driving radiodermatitis. EMBO Mol Med. 2022;14:e15653 pubmed publisher
Czaya B, Heitman K, Campos I, Yanucil C, Kentrup D, Westbrook D, et al. Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling. elife. 2022;11: pubmed publisher
Suzuki S, Toyoma S, Kawasaki Y, Yamada T. Irradiated fibroblasts increase interleukin-6 expression and induce migration of head and neck squamous cell carcinoma. PLoS ONE. 2022;17:e0262549 pubmed publisher
Luo Y, Yao F, Hu X, Li Y, Chen Y, Li Z, et al. M1 macrophages impair tight junctions between endothelial cells after spinal cord injury. Brain Res Bull. 2022;180:59-72 pubmed publisher
Porcher L, Bruckmeier S, Burbano S, Finnell J, Gorny N, Klett J, et al. Aging triggers an upregulation of a multitude of cytokines in the male and especially the female rodent hippocampus but more discrete changes in other brain regions. J Neuroinflammation. 2021;18:219 pubmed publisher
Westermann L, Baranowsky A, Di Lorenzo G, Danyukova T, Soul J, Schwartz J, et al. Transgenic inhibition of interleukin-6 trans-signaling does not prevent skeletal pathologies in mucolipidosis type II mice. Sci Rep. 2021;11:3556 pubmed publisher
Martos Rus C, Katz Greenberg G, Lin Z, Serrano E, Whitaker Menezes D, Domingo Vidal M, et al. Macrophage and adipocyte interaction as a source of inflammation in kidney disease. Sci Rep. 2021;11:2974 pubmed publisher
Zhang M, Gao J, Zhao X, Zhao M, Ma D, Zhang X, et al. p38α in macrophages aggravates arterial endothelium injury by releasing IL-6 through phosphorylating megakaryocytic leukemia 1. Redox Biol. 2021;38:101775 pubmed publisher
Tyrrell D, Blin M, Song J, Wood S, Goldstein D. Aging Impairs Mitochondrial Function and Mitophagy and Elevates Interleukin 6 Within the Cerebral Vasculature. J Am Heart Assoc. 2020;9:e017820 pubmed publisher
Tanner M, Thomas T, Maitz C, Grisanti L. β2-Adrenergic Receptors Increase Cardiac Fibroblast Proliferation Through the Gαs/ERK1/2-Dependent Secretion of Interleukin-6. Int J Mol Sci. 2020;21: pubmed publisher
Xiong L, Zhao K, Cao Y, Guo H, Pan J, Yang X, et al. Linking skeletal muscle aging with osteoporosis by lamin A/C deficiency. PLoS Biol. 2020;18:e3000731 pubmed publisher
Li Z, Zhang C, Du J, Zhao J, Shi M, Jin M, et al. Adipocytes promote tumor progression and induce PD-L1 expression via TNF-α/IL-6 signaling. Cancer Cell Int. 2020;20:179 pubmed publisher
Kuo C, Chen J, Lin L, Schmid Schonbein G, Chien S, Huang P, et al. Inhibition of Serine Protease Activity Protects Against High Fat Diet-Induced Inflammation and Insulin Resistance. Sci Rep. 2020;10:1725 pubmed publisher
Takagaki Y, Lee S, Dongqing Z, Kitada M, Kanasaki K, Koya D. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis. Autophagy. 2020;:1-10 pubmed publisher
Eckert N, Werth K, Willenzon S, Tan L, Forster R. B cell hyperactivation in an Ackr4-deficient mouse strain is not caused by lack of ACKR4 expression. J Leukoc Biol. 2020;107:1155-1166 pubmed publisher
Kim D, Choi J, Jo I, Kim M, Lee H, Hong S, et al. Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway. Mol Med Rep. 2020;21:258-266 pubmed publisher
Gardner G, Travers J, Qian J, Liu G, Haghighi K, Robbins N, et al. Phosphorylation of Hsp20 Promotes Fibrotic Remodeling and Heart Failure. JACC Basic Transl Sci. 2019;4:188-199 pubmed publisher
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, et al. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC. Oncogene. 2019;38:4366-4383 pubmed publisher
Tang Y, Xiong S, Yu P, Liu F, Cheng L. Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210. Cell Rep. 2018;24:1355-1362.e3 pubmed publisher
Mitani T, Takaya T, Harada N, Katayama S, Yamaji R, Nakamura S, et al. Theophylline suppresses interleukin-6 expression by inhibiting glucocorticoid receptor signaling in pre-adipocytes. Arch Biochem Biophys. 2018;646:98-106 pubmed publisher
Akama T, Chun T. Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells. J Biol Chem. 2018;293:6603-6610 pubmed publisher
Sun X, Gupta K, Wu B, Zhang D, Yuan B, Zhang X, et al. Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulating adipose stromal interleukin 6 production in mice. J Biol Chem. 2018;293:2841-2849 pubmed publisher
Pang Z, Junkins R, MacNeil A, McCormick C, Cheng Z, Chen W, et al. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection. J Leukoc Biol. 2017;: pubmed publisher
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3. Cell Physiol Biochem. 2017;42:1961-1972 pubmed publisher
Zaman T, Arimochi H, Maruyama S, Ishifune C, Tsukumo S, Kitamura A, et al. Notch Balances Th17 and Induced Regulatory T Cell Functions in Dendritic Cells by Regulating Aldh1a2 Expression. J Immunol. 2017;199:1989-1997 pubmed publisher
Ohkura S, Usui S, Takashima S, Takuwa N, Yoshioka K, Okamoto Y, et al. Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6. PLoS ONE. 2017;12:e0182329 pubmed publisher
Zhang L, Tian Z, Li W, Wang X, Man Z, Sun S. Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis. Biosci Rep. 2017;: pubmed publisher
Chehboun S, Labrecque Carbonneau J, Pasquin S, Meliani Y, Meddah B, Ferlin W, et al. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling. J Biol Chem. 2017;292:6644-6656 pubmed publisher
Lazzarini E, Balbi C, Altieri P, Pfeffer U, Gambini E, Canepa M, et al. The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity. Sci Rep. 2016;6:29994 pubmed publisher
Lim J, Im K, Lee E, Kim N, Nam Y, Jeon Y, et al. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis. Sci Rep. 2016;6:26851 pubmed publisher
Caetano M, Zhang H, Cumpian A, Gong L, Unver N, Ostrin E, et al. IL6 Blockade Reprograms the Lung Tumor Microenvironment to Limit the Development and Progression of K-ras-Mutant Lung Cancer. Cancer Res. 2016;76:3189-99 pubmed publisher
Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, et al. KIM-1-mediated phagocytosis reduces acute injury to the kidney. J Clin Invest. 2015;125:1620-36 pubmed publisher
Chandler R, Damrauer J, Raab J, Schisler J, Wilkerson M, Didion J, et al. Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling. Nat Commun. 2015;6:6118 pubmed publisher
Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, et al. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med. 2015;7:211-26 pubmed publisher
Volonte D, Zou H, Bartholomew J, Liu Z, Morel P, Galbiati F. Oxidative stress-induced inhibition of Sirt1 by caveolin-1 promotes p53-dependent premature senescence and stimulates the secretion of interleukin 6 (IL-6). J Biol Chem. 2015;290:4202-14 pubmed publisher
Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, et al. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis. Nature. 2014;515:130-3 pubmed publisher
Ke F, Zhang L, Liu Z, Liu J, Yan S, Xu Z, et al. Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels. Stem Cells. 2014;32:2799-810 pubmed publisher
Zhao Y, Xiao X, Frank S, Lin H, Xia Y. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-?, and IL-1?. Am J Physiol Endocrinol Metab. 2014;307:E186-98 pubmed publisher
Kai T, Yamazaki T, Arai S, Miyazaki T. Stabilization and augmentation of circulating AIM in mice by synthesized IgM-Fc. PLoS ONE. 2014;9:e97037 pubmed publisher
Ramkhelawon B, Hennessy E, Ménager M, Ray T, Sheedy F, Hutchison S, et al. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med. 2014;20:377-84 pubmed publisher
Kim M, Gorouhi F, Ramirez S, Granick J, Byrne B, Soulika A, et al. Catecholamine stress alters neutrophil trafficking and impairs wound healing by ?2-adrenergic receptor-mediated upregulation of IL-6. J Invest Dermatol. 2014;134:809-817 pubmed publisher
Gomez M, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, et al. Basophils control T-cell responses and limit disease activity in experimental murine colitis. Mucosal Immunol. 2014;7:188-99 pubmed publisher
Lipina T, Zai C, Hlousek D, Roder J, Wong A. Maternal immune activation during gestation interacts with Disc1 point mutation to exacerbate schizophrenia-related behaviors in mice. J Neurosci. 2013;33:7654-66 pubmed publisher
Junkins R, MacNeil A, Wu Z, McCormick C, Lin T. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol. 2013;190:5178-86 pubmed publisher
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, et al. Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine. 2012;58:169-77 pubmed publisher
Roy M, Richard J, Dumas A, Vallières L. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. J Neuroinflammation. 2012;9:18 pubmed publisher
Gessner M, Werner J, Lilly L, Nelson M, Metz A, Dunaway C, et al. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Infect Immun. 2012;80:410-7 pubmed publisher
Ma Y, Aymeric L, Locher C, Mattarollo S, Delahaye N, Pereira P, et al. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy. J Exp Med. 2011;208:491-503 pubmed publisher
Wu W, Mehta H, Chakrabarty K, Booth J, Duggan E, Patel K, et al. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J Immunol. 2009;183:5799-806 pubmed publisher
Osuchowski M, Connett J, Welch K, Granger J, Remick D. Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis. Crit Care Med. 2009;37:1567-73 pubmed publisher
Wieland C, van der Windt G, Florquin S, McKenzie A, van der Poll T. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect. 2009;11:524-30 pubmed publisher
Wieland C, van der Windt G, Wiersinga W, Florquin S, van der Poll T. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology. 2008;125:272-9 pubmed publisher
Muranski P, Boni A, Antony P, Cassard L, Irvine K, Kaiser A, et al. Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood. 2008;112:362-73 pubmed publisher
Smith S, Li J, Garbett K, Mirnics K, Patterson P. Maternal immune activation alters fetal brain development through interleukin-6. J Neurosci. 2007;27:10695-702 pubmed
Taki N, Tatro J, Lowe R, Goldberg V, Greenfield E. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Bone. 2007;40:1276-83 pubmed
Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, et al. A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model. J Mol Cell Cardiol. 2006;41:885-92 pubmed
Osuchowski M, Welch K, Siddiqui J, Remick D. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J Immunol. 2006;177:1967-74 pubmed
An H, Jeong H, Um J, Kim H, Hong S. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol. 2006;106:418-24 pubmed
Osuchowski M, Siddiqui J, Copeland S, Remick D. Sequential ELISA to profile multiple cytokines from small volumes. J Immunol Methods. 2005;302:172-81 pubmed
Ellaban E, Bolgos G, Remick D. Selective macrophage suppression during sepsis. Cell Immunol. 2004;231:103-11 pubmed
Fredj S, Bescond J, Louault C, Delwail A, Lecron J, Potreau D. Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation. J Cell Physiol. 2005;204:428-36 pubmed
Miyamoto M, Prause O, Sjöstrand M, Laan M, Lotvall J, Linden A. Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways. J Immunol. 2003;170:4665-72 pubmed
Pechová J, Stepánová G, Kovar L, Kopecky J. Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Folia Parasitol (Praha). 2002;49:153-9 pubmed
image
image 1 :
R&D Systems MAB406-100 image 1
Detection of Recombinant Mouse IL-6 antibody by Western Blot.
product information
brand :
R&D Systems
master code :
MAB406
SKU :
MAB406-100
product name :
IL-6 Antibody
description :
The Mouse IL-6 Antibody from R&D Systems is a rat monoclonal antibody to IL-6. This antibody reacts with mouse. The Mouse IL-6 Antibody has been validated for the following applications: Western Blot, Neutralization, ELISA Capture (Matched Antibody Pair).
target :
IL-6
category :
Primary Antibodies
unit sizes :
100 ug (also 25 ug, 500 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
clonality :
Monoclonal
clone :
MP5-20F3
conjugate :
Unconjugated
host :
Rat
immunogen :
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Mouse
specificity :
Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-6, recombinant porcine IL-6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.
gene symbol :
IL6
endotoxin note :
<0.10 EU per 1 µg of the antibody by the LAL method.
applications :
Western Blot, Neutralization, ELISA Capture (Matched Antibody Pair)
USD :
453 USD
alt names :
B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.