product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Fibroblast Activation Protein alpha /FAP Antibody
catalog :
MAB3715-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
479 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
427819
reactivity :
human
application :
ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section
more info or order :
R&D Systems product webpage
citations: 19
Reference
Komoto S, Noma K, Kato T, Kobayashi T, Nishiwaki N, Narusaka T, et al. Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models. Cancers (Basel). 2023;15: pubmed publisher
Jang E, Shin M, Kim H, Lim J, Lee J, Park J, et al. Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells. Exp Mol Med. 2023;55:974-986 pubmed publisher
Jin J, Barnett J, Krishnamachary B, Mironchik Y, Luo C, Kobayashi H, et al. Evaluating near-infrared photoimmunotherapy for targeting fibroblast activation protein-α expressing cells in vitro and in vivo. Cancer Sci. 2023;114:236-246 pubmed publisher
Sato H, Noma K, Ohara T, Kawasaki K, Akai M, Kobayashi T, et al. Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment. Sci Rep. 2022;12:20152 pubmed publisher
Magagna I, Gourdin N, Kieffer Y, Licaj M, Mhaidly R, Andre P, et al. CD73-Mediated Immunosuppression Is Linked to a Specific Fibroblast Population That Paves the Way for New Therapy in Breast Cancer. Cancers (Basel). 2021;13: pubmed publisher
Katsube R, Noma K, Ohara T, Nishiwaki N, Kobayashi T, Komoto S, et al. Fibroblast activation protein targeted near infrared photoimmunotherapy (NIR PIT) overcomes therapeutic resistance in human esophageal cancer. Sci Rep. 2021;11:1693 pubmed publisher
Bonneau C, Eliès A, Kieffer Y, Bourachot B, Ladoire S, Pelon F, et al. A subset of activated fibroblasts is associated with distant relapse in early luminal breast cancer. Breast Cancer Res. 2020;22:76 pubmed publisher
Layton T, Williams L, McCann F, Zhang M, Fritzsche M, Colin York H, et al. Cellular census of human fibrosis defines functionally distinct stromal cell types and states. Nat Commun. 2020;11:2768 pubmed publisher
Kieffer Y, Hocine H, Gentric G, Pelon F, Bernard C, Bourachot B, et al. Single-Cell Analysis Reveals Fibroblast Clusters Linked to Immunotherapy Resistance in Cancer. Cancer Discov. 2020;10:1330-1351 pubmed publisher
Xiang H, Ramil C, Hai J, Zhang C, Wang H, Watkins A, et al. Cancer-Associated Fibroblasts Promote Immunosuppression by Inducing ROS-Generating Monocytic MDSCs in Lung Squamous Cell Carcinoma. Cancer Immunol Res. 2020;8:436-450 pubmed publisher
Pelon F, Bourachot B, Kieffer Y, Magagna I, Mermet Meillon F, Bonnet I, et al. Cancer-associated fibroblast heterogeneity in axillary lymph nodes drives metastases in breast cancer through complementary mechanisms. Nat Commun. 2020;11:404 pubmed publisher
Scott E, Jacobus E, Lyons B, FROST S, Freedman J, Dyer A, et al. Bi- and tri-valent T cell engagers deplete tumour-associated macrophages in cancer patient samples. J Immunother Cancer. 2019;7:320 pubmed publisher
Nguyen M, De Ninno A, Mencattini A, Mermet Meillon F, Fornabaio G, Evans S, et al. Dissecting Effects of Anti-cancer Drugs and Cancer-Associated Fibroblasts by On-Chip Reconstitution of Immunocompetent Tumor Microenvironments. Cell Rep. 2018;25:3884-3893.e3 pubmed publisher
Witek M, Aufforth R, Wang H, Kamande J, Jackson J, Pullagurla S, et al. Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precis Oncol. 2017;1: pubmed publisher
Costa A, Kieffer Y, Scholer Dahirel A, Pelon F, Bourachot B, Cardon M, et al. Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer. Cancer Cell. 2018;33:463-479.e10 pubmed publisher
Freedman J, Hagel J, Scott E, Psallidas I, Gupta A, Spiers L, et al. Oncolytic adenovirus expressing bispecific antibody targets T-cell cytotoxicity in cancer biopsies. EMBO Mol Med. 2017;9:1067-1087 pubmed publisher
Liao Y, Xing S, Xu B, Liu W, Zhang G. Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma. Oncotarget. 2017;8:30050-30062 pubmed publisher
Simkova D, Kharaishvili G, Korinkova G, Ozdian T, Suchánková Kleplová T, Soukup T, et al. The dual role of asporin in breast cancer progression. Oncotarget. 2016;7:52045-52060 pubmed publisher
Majety M, Pradel L, Gies M, Ries C. Fibroblasts Influence Survival and Therapeutic Response in a 3D Co-Culture Model. PLoS ONE. 2015;10:e0127948 pubmed publisher
product information
brand :
R&D Systems
master code :
MAB3715
SKU :
MAB3715-100
product name :
Human Fibroblast Activation Protein alpha /FAP Antibody
unit size :
100 ug (also 25 ug, 500 ug)
seo description :
The Human Fibroblast Activation Protein alpha /FAP Antibody from R&D Systems is a mouse monoclonal antibody to Fibroblast Activation Protein alpha/FAP. This antibody reacts with human,xenograft. The Human Fibroblast Activation Protein alpha /FAP Antibody has been validated for the following applications: Flow Cytometry,Cell Capture,FACS,Functional Assay,Cell Culture,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),CyTOF-ready.
target :
Fibroblast Activation Protein alpha/FAP
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
427819
conjugate :
Unconjugated
dilution :
Flow Cytometry 0.25 ug/10^6 cells, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
host :
Mouse
immunogen :
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP, Leu26-Asp760, Accession # Q12884
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Xenograft
specificity :
Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 and DPP4 is observed.
gene symbol :
FAP
top caption :
Detection of Fibroblast Activation Protein a/FAP antibody in WI-38 Human Cell Line antibody by Flow Cytometry.
accessionNumbers :
Q12884
applications :
ELISA Capture (Matched Antibody Pair),CyTOF-ready,Cell Capture,FACS,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunocytochemistry,Functional Assay,Cell Culture
USD :
479 USD
alt names :
170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast activation protein alpha, fibroblast activation protein, alpha, Integral membrane serine protease, seprase, vibronectin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.