Human Myocilin Antibody | R&D Systems MAB3446 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human Myocilin Antibody

catalog :

MAB3446

quantity :

100 ug (also 25 ug)

price :

459 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

297817

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

R&D Systems product webpage

citations: 14

Reference
Scelsi H, Hill K, Barlow B, Martin M, Lieberman R. Quantitative differentiation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability. Dis Model Mech. 2023;16: pubmed publisher
Saccuzzo E, Martin M, Hill K, Ma M, Ku Y, Lieberman R. Calcium dysregulation potentiates wild-type myocilin misfolding: implications for glaucoma pathogenesis. J Biol Inorg Chem. 2022;27:553-564 pubmed publisher
Jasien J, Read A, van Batenburg Sherwood J, Perkumas K, Ethier C, Stamer W, et al. Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri). Invest Ophthalmol Vis Sci. 2022;63:21 pubmed publisher
Martin M, Huard D, Guerrero Ferreira R, Desai I, Barlow B, Lieberman R. Molecular architecture and modifications of full-length myocilin. Exp Eye Res. 2021;211:108729 pubmed publisher
Patterson Orazem A, Qerqez A, Azouz L, Ma M, Hill S, Ku Y, et al. Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin. J Biol Chem. 2021;297:101067 pubmed publisher
Xiong S, Kumar A, Tian S, Taher E, Yang E, Kinchington P, et al. Stem cell transplantation rescued a primary open-angle glaucoma mouse model. elife. 2021;10: pubmed publisher
Haribalaganesh R, Gowri Priya C, Sharmila R, Krishnadas S, Muthukkaruppan V, Willoughby C, et al. Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes. Sci Rep. 2021;11:605 pubmed publisher
Staverosky J, Dhamodaran K, Acott T, Raghunathan V, Vranka J. Isolation and Characterization of primary human trabecular meshwork cells from segmental flow regions: New tools for understanding segmental flow. Exp Eye Res. 2020;197:108046 pubmed publisher
Hill S, Kwon M, Martin M, Suntharalingam A, Hazel A, Dickey C, et al. Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between benign and glaucoma-causing mutations. J Biol Chem. 2019;294:12717-12728 pubmed publisher
Aroca Aguilar J, Fern xe1 ndez Navarro A, Onta xf1 xf3 n J, Coca Prados M, Escribano J. Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers. PLoS ONE. 2018;13:e0209364 pubmed publisher
Patterson Orazem A, Hill S, Fautsch M, Lieberman R. Epitope mapping of commercial antibodies that detect myocilin. Exp Eye Res. 2018;173:109-112 pubmed publisher
Keller K, Bhattacharya S, Borrás T, Brunner T, Chansangpetch S, Clark A, et al. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res. 2018;171:164-173 pubmed publisher
Suntharalingam A, Abisambra J, O Leary J, Koren J, Zhang B, Joe M, et al. Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism. J Biol Chem. 2012;287:40661-9 pubmed publisher
Joe M, Tomarev S. Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis. Am J Pathol. 2010;176:2880-90 pubmed publisher

product information

master code :

MAB3446

SKU :

MAB3446

product name :

Human Myocilin Antibody

unit size :

100 ug (also 25 ug)

description :

The Human Myocilin Antibody from R&D Systems is a mouse monoclonal antibody to Myocilin. This antibody reacts with human,tree shrew. The Human Myocilin Antibody has been validated for the following applications: Western Blot,Immunoprecipitation,Immunohistochemistry.

target :

Myocilin

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

297817

concentration :

LYOPH

conjugate :

Unconjugated

host :

Mouse

immunogen :

Mouse myeloma cell line NS0-derived recombinant human Myocilin, Arg33-Met504, Accession # Q99972

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Tree Shrew

specificity :

Detects human Myocilin in direct ELISAs. In direct ELISAs, 75% cross-reactivity with recombinant mouse Myocilin is observed.

gene symbol :

MYOC

accessionNumbers :

Q99972

applications :

Immunoprecipitation,Western Blot,Immunohistochemistry

USD :

459 USD

alt names :

GLC1A, GLC1Amyocilin, GPOA, JOAG, JOAG1, MYOC, myocilin, trabecular meshwork inducible glucocorticoid response, TIGR, TIGRmutated trabecular meshwork-induced glucocorticoid response protein, Trabecular meshwork-induced glucocorticoid response protein

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage