product summary
Loading...
company name :
R&D Systems
product type :
antibody
product name :
Human CXCR2/IL-8RB Antibody
catalog :
MAB331-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
469 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
48311
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 66
Reference
Zhao C, Wang D, Li Z, Zhang Z, Xu Y, Liu J, et al. IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer. Int Immunopharmacol. 2023;121:110457 pubmed publisher
Tanaka R, Kimura K, Eguchi S, Ohira G, Tanaka S, Amano R, et al. Interleukin-8 produced from cancer-associated fibroblasts suppresses proliferation of the OCUCh-LM1 cancer cell line. BMC Cancer. 2022;22:748 pubmed publisher
Tsujio G, Maruo K, Yamamoto Y, Sera T, Sugimoto A, Kasashima H, et al. Significance of tumor heterogeneity of p-Smad2 and c-Met in HER2-positive gastric carcinoma with lymph node metastasis. BMC Cancer. 2022;22:598 pubmed publisher
Yamamoto Y, Sugimoto A, Maruo K, Tsujio G, Sera T, Kushiyama S, et al. CXCR2 signaling might have a tumor-suppressive role in patients with cholangiocarcinoma. PLoS ONE. 2022;17:e0266027 pubmed publisher
Freitag N, Baston Buest D, Kruessel J, Markert U, Fehm T, Bielfeld A. Eutopic endometrial immune profile of infertility-patients with and without endometriosis. J Reprod Immunol. 2022;150:103489 pubmed publisher
Muldur S, Vadysirisack D, Ragunathan S, Tang Y, Ricardo A, Sayegh C, et al. Human Neutrophils Respond to Complement Activation and Inhibition in Microfluidic Devices. Front Immunol. 2021;12:777932 pubmed publisher
Gu xe9 rard A, Laurent V, Fromont G, Est xe8 ve D, Gilhodes J, Bonnelye E, et al. The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes. Int J Mol Sci. 2021;22: pubmed publisher
Ali S, Borin T, Piranlioglu R, Ara R, Lebedyeva I, Angara K, et al. Changes in the tumor microenvironment and outcome for TME-targeting therapy in glioblastoma: A pilot study. PLoS ONE. 2021;16:e0246646 pubmed publisher
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
Hoogendijk A, Pourfarzad F, Aarts C, Tool A, Hiemstra I, Grassi L, et al. Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming. Cell Rep. 2019;29:2505-2519.e4 pubmed publisher
Caccuri F, Bugatti A, Corbellini S, Roversi S, Zani A, Mazzuca P, et al. The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3). Int J Mol Sci. 2019;20: pubmed publisher
Huang C, Cheng K, Hsieh Y, Lin W, Cheng C, Yuan S, et al. Use of syngeneic cells expressing membrane-bound GM-CSF as an adjuvant to induce antibodies against native multi-pass transmembrane protein. Sci Rep. 2019;9:9931 pubmed publisher
Syu G, Wang S, Ma G, Liu S, Pearce D, Prakash A, et al. Development and application of a high-content virion display human GPCR array. Nat Commun. 2019;10:1997 pubmed publisher
Kim H, Lee S, Lee S, Oh B, Kim J, Kim J, et al. A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells. Stem Cells. 2019;37:77-88 pubmed publisher
Angara K, Borin T, Rashid M, Lebedyeva I, Ara R, Lin P, et al. CXCR2-Expressing Tumor Cells Drive Vascular Mimicry in Antiangiogenic Therapy-Resistant Glioblastoma. Neoplasia. 2018;20:1070-1082 pubmed publisher
Sharma I, Singh A, Siraj F, Saxena S. IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma. J Biomed Sci. 2018;25:62 pubmed publisher
Kwon E, Todorova K, Wang J, Horos R, Lee K, Neel V, et al. The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional level. Nat Commun. 2018;9:1734 pubmed publisher
Yang L, Herrera J, Gilbertsen A, Xia H, Smith K, Benyumov A, et al. IL-8 mediates idiopathic pulmonary fibrosis mesenchymal progenitor cell fibrogenicity. Am J Physiol Lung Cell Mol Physiol. 2018;314:L127-L136 pubmed publisher
Mazzuca P, Marsico S, Schulze K, Mitola S, Pils M, Giagulli C, et al. Role of Autophagy in HIV-1 Matrix Protein p17-Driven Lymphangiogenesis. J Virol. 2017;91: pubmed publisher
Kasashima H, Yashiro M, Nakamae H, Masuda G, Kinoshita H, Morisaki T, et al. Clinicopathologic significance of the CXCL1-CXCR2 axis in the tumor microenvironment of gastric carcinoma. PLoS ONE. 2017;12:e0178635 pubmed publisher
Loi A, Hoonhorst S, van Aalst C, Langereis J, Kamp V, Sluis Eising S, et al. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients. Respir Res. 2017;18:100 pubmed publisher
Qaqish A, Huang D, Chen C, Zhang Z, Wang R, Li S, et al. Adoptive Transfer of Phosphoantigen-Specific ?? T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates. J Immunol. 2017;198:4753-4763 pubmed publisher
Xu W, Li B, Guan X, Chung S, Wang Y, Yip Y, et al. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression. Nat Commun. 2017;8:14399 pubmed publisher
Alaridah N, Lutay N, Tenland E, Rönnholm A, Hallgren O, Puthia M, et al. Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs. J Innate Immun. 2017;9:318-329 pubmed publisher
Williams A, José R, Mercer P, Brealey D, Parekh D, Thickett D, et al. Evidence for chemokine synergy during neutrophil migration in ARDS. Thorax. 2017;72:66-73 pubmed publisher
Sun K, Sun G, Wu Y, Ko B, Hsu H, Wu S. TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma. J Cell Mol Med. 2016;20:2020-2028 pubmed publisher
Laurent V, Guérard A, Mazerolles C, Le Gonidec S, Toulet A, Nieto L, et al. Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity. Nat Commun. 2016;7:10230 pubmed publisher
Li L, Xu L, Yan J, Zhen Z, Ji Y, Liu C, et al. CXCR2-CXCL1 axis is correlated with neutrophil infiltration and predicts a poor prognosis in hepatocellular carcinoma. J Exp Clin Cancer Res. 2015;34:129 pubmed publisher
Kopesky P, Tiedemann K, Alkekhia D, Zechner C, Millard B, Schoeberl B, et al. Autocrine signaling is a key regulatory element during osteoclastogenesis. Biol Open. 2014;3:767-76 pubmed publisher
Al Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol. 2014;193:1416-26 pubmed publisher
Watari K, Shibata T, Kawahara A, Sata K, Nabeshima H, Shinoda A, et al. Tumor-derived interleukin-1 promotes lymphangiogenesis and lymph node metastasis through M2-type macrophages. PLoS ONE. 2014;9:e99568 pubmed publisher
Carrega P, Bonaccorsi I, Di Carlo E, Morandi B, Paul P, Rizzello V, et al. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol. 2014;192:3805-15 pubmed publisher
Caccuri F, Giagulli C, Reichelt J, Martorelli D, Marsico S, Bugatti A, et al. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth. J Virol. 2014;88:5706-17 pubmed publisher
Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, et al. CXCL1/CXCL8 (GRO?/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro. Am J Physiol Endocrinol Metab. 2014;306:E1077-84 pubmed publisher
Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, et al. The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma. BMC Cancer. 2014;14:149 pubmed publisher
Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, et al. Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8. Int J Clin Exp Pathol. 2014;7:575-83 pubmed
Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, et al. Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients. Eur J Immunol. 2014;44:420-30 pubmed publisher
Infanger D, Cho Y, Lopez B, Mohanan S, Liu S, Gursel D, et al. Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche. Cancer Res. 2013;73:7079-89 pubmed publisher
Dong R, Dong K, Wang X, Chen G, Shen C, Zheng S. Interleukin-33 overexpression is associated with gamma-glutamyl transferase in biliary atresia. Cytokine. 2013;61:433-7 pubmed publisher
Ruan J, Liao Y, Lua I, Li M, Hsu C, Chen J. Human pituitary tumor-transforming gene 1 overexpression reinforces oncogene-induced senescence through CXCR2/p21 signaling in breast cancer cells. Breast Cancer Res. 2012;14:R106 pubmed publisher
Schulz C, Stoelcker B, Ruhland B, Jentsch N, Steege A. Bronchoepithelial expression of CXCR1 and CXCR2 does not facilitate transepithelial migration of neutrophils. Respiration. 2012;84:108-16 pubmed publisher
Guo X, Liu G, Schauer I, Yang G, Mercado Uribe I, Yang F, et al. Overexpression of the β subunit of human chorionic gonadotropin promotes the transformation of human ovarian epithelial cells and ovarian tumorigenesis. Am J Pathol. 2011;179:1385-93 pubmed publisher
Li A, King J, Moro A, Sugi M, Dawson D, Kaplan J, et al. Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer. Am J Pathol. 2011;178:1340-9 pubmed publisher
Yang G, Rosen D, Liu G, Yang F, Guo X, Xiao X, et al. CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis. Clin Cancer Res. 2010;16:3875-86 pubmed publisher
Weiss N, Deboux C, Chaverot N, Miller F, Baron van Evercooren A, Couraud P, et al. IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells. J Neuroimmunol. 2010;223:131-4 pubmed publisher
Mintz R, Garty B, Meshel T, Marcus N, Katanov C, Cohen Hillel E, et al. Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients. Immunol Lett. 2010;130:97-106 pubmed publisher
Reikvam H, Hatfield K, Oyan A, Kalland K, Kittang A, Bruserud O. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur J Haematol. 2010;84:239-51 pubmed publisher
van der Veen B, Petersen A, Belperio J, Satchell S, Mathieson P, Molema G, et al. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis. Clin Exp Immunol. 2009;158:143-53 pubmed publisher
Singh S, Nannuru K, Sadanandam A, Varney M, Singh R. CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion. Br J Cancer. 2009;100:1638-46 pubmed publisher
Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, et al. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche. Cancer Res. 2008;68:9754-62 pubmed publisher
Rowe S, Jackson P, Liu G, Hardison M, Livraghi A, Solomon G, et al. Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Am J Respir Crit Care Med. 2008;178:822-31 pubmed publisher
Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, et al. Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina. Invest Ophthalmol Vis Sci. 2008;49:4578-89 pubmed publisher
Gouwy M, Struyf S, Noppen S, Schutyser E, Springael J, Parmentier M, et al. Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events. Mol Pharmacol. 2008;74:485-95 pubmed publisher
Gaggar A, Jackson P, Noerager B, O Reilly P, McQuaid D, Rowe S, et al. A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation. J Immunol. 2008;180:5662-9 pubmed
Tsai W, Hsu H, Lin C, Ho C, Kou Y. Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells. Crit Care Med. 2007;35:879-85 pubmed
Smith C, Damås J, Otterdal K, Øie E, Sandberg W, Yndestad A, et al. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J Am Coll Cardiol. 2006;48:1591-9 pubmed
Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon Tasson L, et al. Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression. Leuk Res. 2007;31:591-8 pubmed
Haas C, Martinez R, Attia N, Haines G, Campbell P, Koch A. Chemokine receptor expression in rat adjuvant-induced arthritis. Arthritis Rheum. 2005;52:3718-30 pubmed
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, et al. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J Immunol. 2005;175:6177-89 pubmed
Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, et al. The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium. J Neuroimmunol. 2005;161:49-60 pubmed
Traves S, Smith S, Barnes P, Donnelly L. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J Leukoc Biol. 2004;76:441-50 pubmed
Annels N, Willemze A, van der Velden V, Faaij C, van Wering E, Sie Go D, et al. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood. 2004;103:2806-8 pubmed
Keane M, Donnelly S, Belperio J, Goodman R, Dy M, Burdick M, et al. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. J Immunol. 2002;169:6515-21 pubmed
Huber T, Reinhardt H, Exner M, Burger J, Kerjaschki D, Saleem M, et al. Expression of functional CCR and CXCR chemokine receptors in podocytes. J Immunol. 2002;168:6244-52 pubmed
Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, et al. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol. 2001;166:6477-82 pubmed
Frendeus B, Godaly G, Hang L, Karpman D, Lundstedt A, Svanborg C. Interleukin 8 receptor deficiency confers susceptibility to acute experimental pyelonephritis and may have a human counterpart. J Exp Med. 2000;192:881-90 pubmed
product information
brand :
R&D Systems
master code :
MAB331
SKU :
MAB331-100
product name :
Human CXCR2/IL-8RB Antibody
unit size :
100 ug (also 25 ug, 500 ug)
seo description :
The Human CXCR2/IL-8RB Antibody from R&D Systems is a mouse monoclonal antibody to CXCR2/IL-8RB. This antibody reacts with guinea pig,human,mouse,rabbit,rat. The Human CXCR2/IL-8RB Antibody has been validated for the following applications: Bioassay,Flow Cytometry,ELISA Development,Western Blot,Neutralization,Functional Assay,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready.
target :
CXCR2/IL-8RB
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
48311
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 8-25 ug/mL, Neutralization 1-5 ug/mL, CyTOF-ready
host :
Mouse
immunogen :
NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB, Met1-Leu355, Accession # AAB25880
isotype :
IgG2a
purity :
Protein A or G purified from ascites
species :
Guinea Pig,Human,Mouse,Rabbit,Rat
specificity :
Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
gene symbol :
CXCR2
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of CXCR2/IL-8 RB antibody in Human Blood Granulocytes antibody by Flow Cytometry.
accessionNumbers :
AAB25880
applications :
CyTOF-ready,Bioassay,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,Functional Assay
extended description :
Clone 48311 was used by HLDA to establish CD designation
USD :
469 USD
product details :
Clone 48311 was used by HLDA to establish CD designation
alt names :
CD182, CD182 antigen, CDw128b, chemokine (C-X-C motif) receptor 2, chemokine (CXC) receptor 2, CMKAR2, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CXCR2 gene for IL8 receptor type B, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8 RB, IL-8 receptor type 2, IL-8R B, IL8R2, IL8RA, IL8RB, interleukin 8 receptor B, interleukin 8 receptor type 2, interleukin-8 receptor type B
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.