This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
antibody
product name :
Human/Primate VEGF Antibody
catalog :
MAB293
quantity :
100 ug (also 25 ug, 500 ug)
price :
369 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
26503
reactivity :
human, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization
citations: 51
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; bovine; loading ...; fig 1a
  • western blot; bovine; loading ...; fig 1a
Stassi A, Gasser F, Velázquez M, Belotti E, Gareis N, Rey F, et al. Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries. Theriogenology. 2019;138:52-65 pubmed publisher
  • immunocytochemistry; human; 1:500; tbl s1
Yasui T, Uezono N, Nakashima H, Noguchi H, Matsuda T, Noda Andoh T, et al. Hypoxia Epigenetically Confers Astrocytic Differentiation Potential on Human Pluripotent Cell-Derived Neural Precursor Cells. Stem Cell Reports. 2017;8:1743-1756 pubmed publisher
Oh H, Kwak J, Kim B, Jin H, Park W, Choi S, et al. Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth. Cells. 2020;9: pubmed publisher
Ren J, Gu C, Yang Y, Xue J, Sun Y, Jian F, et al. TSP-1 is downregulated and inversely correlates with miR-449c expression in Cushing's disease. J Cell Mol Med. 2019;23:4097-4110 pubmed publisher
Zizza P, Dinami R, Porru M, Cingolani C, Salvati E, Rizzo A, et al. TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment. Nucleic Acids Res. 2019;47:3365-3382 pubmed publisher
Kim H, Lee S, Lee S, Oh B, Kim J, Kim J, et al. A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells. Stem Cells. 2019;37:77-88 pubmed publisher
Tang M, Yue P, Ip P, Huang R, Lai H, Cheung A, et al. Soluble E-cadherin promotes tumor angiogenesis and localizes to exosome surface. Nat Commun. 2018;9:2270 pubmed publisher
Rodriguez Lanzi C, Perdicaro D, Landa M, Fontana A, Antoniolli A, Miatello R, et al. Grape pomace extract induced beige cells in white adipose tissue from rats and in 3T3-L1 adipocytes. J Nutr Biochem. 2018;56:224-233 pubmed publisher
Rao L, De Veirman K, Giannico D, Saltarella I, Desantis V, Frassanito M, et al. Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin® protein MP0250: a preclinical study. Oncotarget. 2018;9:13366-13381 pubmed publisher
Xu X, Cui N, Wang E. Application of an acellular dermal matrix to a rabbit model of oral mucosal defects. Exp Ther Med. 2018;15:2450-2456 pubmed publisher
Gogalic S, Sauer U, Doppler S, Preininger C. Investigating Colorimetric Protein Array Assay Schemes for Detection of Recurrence of Bladder Cancer. Biosensors (Basel). 2018;8: pubmed publisher
McDonald G, Tabellini L, Storer B, Martin P, Lawler R, Rosinski S, et al. Predictive Value of Clinical Findings and Plasma Biomarkers after Fourteen Days of Prednisone Treatment for Acute Graft-versus-host Disease. Biol Blood Marrow Transplant. 2017;23:1257-1263 pubmed publisher
Strickland Marmol L, Brem S, Rojiani A, Rojiani M. Vessel morphometric parameters-correlation with histologic grade and VEGF expression in oligodendroglioma. Am J Cancer Res. 2017;7:973-981 pubmed
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, et al. HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget. 2017;8:28074-28092 pubmed publisher
Katagiri W, Kawai T, Osugi M, Sugimura Wakayama Y, Sakaguchi K, Kojima T, et al. Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells. Maxillofac Plast Reconstr Surg. 2017;39:8 pubmed publisher
Thej C, Ramadasse B, Walvekar A, Majumdar A, Balasubramanian S. Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel®, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product. Stem Cell Res Ther. 2017;8:47 pubmed publisher
Ebrahim N, Leach L. Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms. Placenta. 2016;48:87-98 pubmed publisher
Jing Z, Xu H, Chen X, Zhong Q, Huang J, Zhang Y, et al. The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer. PLoS ONE. 2016;11:e0152789 pubmed publisher
Grün D, Adhikary G, Eckert R. VEGF-A acts via neuropilin-1 to enhance epidermal cancer stem cell survival and formation of aggressive and highly vascularized tumors. Oncogene. 2016;35:4379-87 pubmed publisher
Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, et al. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis. J Biol Chem. 2015;290:22212-24 pubmed publisher
Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, et al. Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke. PLoS ONE. 2015;10:e0124362 pubmed publisher
Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nat Commun. 2014;5:4715 pubmed publisher
Etulain J, Negrotto S, Tribulatti M, Croci D, Carabelli J, Campetella O, et al. Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors. PLoS ONE. 2014;9:e96402 pubmed publisher
Smith A, Bowers S, Stratman A, Davis G. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. PLoS ONE. 2013;8:e85147 pubmed publisher
Townsend K, Spowart J, Huwait H, Eshragh S, West N, Elrick M, et al. Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma. PLoS ONE. 2013;8:e82406 pubmed publisher
Ma X, Hibbert B, McNulty M, Hu T, Zhao X, Ramirez F, et al. Heat shock protein 27 attenuates neointima formation and accelerates reendothelialization after arterial injury and stent implantation: importance of vascular endothelial growth factor up-regulation. FASEB J. 2014;28:594-602 pubmed publisher
Tan J, Prosser H, Vanags L, Monger S, Ng M, Bursill C. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1?. FASEB J. 2014;28:206-17 pubmed publisher
Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll J, Brambilla C, et al. The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo. Oncogene. 2010;29:5392-403 pubmed publisher
Varet J, Douglas S, Gilmartin L, Medford A, Bates D, Harper S, et al. VEGF in the lung: a role for novel isoforms. Am J Physiol Lung Cell Mol Physiol. 2010;298:L768-74 pubmed publisher
Hashizume H, Falcon B, Kuroda T, Baluk P, Coxon A, Yu D, et al. Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth. Cancer Res. 2010;70:2213-23 pubmed publisher
Zhu W, Ou Y, Li Y, Xiao R, Shu M, Zhou Y, et al. A small-molecule triptolide suppresses angiogenesis and invasion of human anaplastic thyroid carcinoma cells via down-regulation of the nuclear factor-kappa B pathway. Mol Pharmacol. 2009;75:812-9 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Allerstorfer S, Sonvilla G, Fischer H, Spiegl Kreinecker S, Gauglhofer C, Setinek U, et al. FGF5 as an oncogenic factor in human glioblastoma multiforme: autocrine and paracrine activities. Oncogene. 2008;27:4180-90 pubmed publisher
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
Ball S, Shuttleworth C, Kielty C. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J Cell Biol. 2007;177:489-500 pubmed
Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal P, Pesce M, et al. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am J Pathol. 2006;169:1167-82 pubmed
Lucerna M, Zernecke A, de Nooijer R, de Jager S, Bot I, van der Lans C, et al. Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment. Blood. 2007;109:122-9 pubmed
Hatfield K, Ryningen A, Corbascio M, Bruserud O. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Int J Cancer. 2006;119:2313-21 pubmed
Rofstad E, Mathiesen B, Kindem K, Galappathi K. Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice. Cancer Res. 2006;66:6699-707 pubmed
Feltis B, Wignarajah D, Zheng L, Ward C, Reid D, Harding R, et al. Increased vascular endothelial growth factor and receptors: relationship to angiogenesis in asthma. Am J Respir Crit Care Med. 2006;173:1201-7 pubmed
Bates D, MacMillan P, Manjaly J, Qiu Y, Hudson S, Bevan H, et al. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term. Clin Sci (Lond). 2006;110:575-85 pubmed
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, et al. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J Immunol. 2005;175:6177-89 pubmed
Rodriguez Porcel M, Zhu X, Chade A, Amores Arriaga B, Caplice N, Ritman E, et al. Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension. Am J Physiol Heart Circ Physiol. 2006;290:H978-84 pubmed
Li L, Lin X, Staver M, Shoemaker A, Semizarov D, Fesik S, et al. Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo. Cancer Res. 2005;65:7249-58 pubmed
Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, et al. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas. Cancer Res. 2005;65:7241-8 pubmed
Tamaki M, Saito R, Ogawa O, Yoshimura N, Ueda T. Possible mechanisms inducing glomerulations in interstitial cystitis: relationship between endoscopic findings and expression of angiogenic growth factors. J Urol. 2004;172:945-8 pubmed
Caine G, Blann A, Stonelake P, Ryan P, Lip G. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur J Clin Invest. 2003;33:883-90 pubmed
Barbour A, Reeder J, Walsh M, Fawcett J, Antalis T, Gotley D. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3. Cancer Res. 2003;63:887-92 pubmed
Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, et al. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Am J Pathol. 2002;160:1405-23 pubmed
Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, et al. Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts. Transplant Proc. 2000;32:987-9 pubmed
Gu J, Adair T. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells. Am J Physiol. 1997;273:H628-33 pubmed
product information
brand :
R&D Systems
master code :
MAB293
SKU :
MAB293-100
product name :
Human/Primate VEGF Antibody
description :
The Human/Primate VEGF Antibody from R&D Systems is a mouse monoclonal antibody to VEGF. This antibody reacts with human, primate. The Human/Primate VEGF Antibody has been validated for the following applications: Western Blot, Neutralization, ELISA Capture (Matched Antibody Pair).
target :
VEGF
category :
Primary Antibodies
unit size :
100 ug (also 25 ug, 500 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
clonality :
Monoclonal
clone :
26503
conjugate :
Unconjugated
host :
Mouse
immunogen :
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165, Ala27-Arg191, Accession # NP_001165097.1
isotype :
IgG2b
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human, Primate
specificity :
Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and recombinant rat (rr)VEGF and no cross-reactivity with recombinant human (rh) VEGF-D is obseved.
gene symbol :
VEGFA
endotoxin note :
<0.10 EU per 1 µg of the antibody by the LAL method.
catalog number base :
MAB293
accessionNumbers :
NP_001165097.1
applications :
Western Blot, Neutralization, ELISA Capture (Matched Antibody Pair)
2020 USD :
339
2021 USD :
369 USD
alt names :
MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.