Human IFN-gamma Antibody | R&D Systems MAB2852-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human IFN-gamma Antibody

catalog :

MAB2852-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

479 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

K3.53

reactivity :

human

application :

western blot, ELISA, neutralization, flow cytometry

more info or order :

R&D Systems product webpage

citations: 19

Reference
Roberts K, Cubitt M, Carlton T, Rodrigues Duarte L, Maggiore L, Chai R, et al. Preclinical development of a bispecific TNFα/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease. Sci Rep. 2021;11:19422 pubmed publisher
Maura D, Elmekki N, Goddard C. The ammonia oxidizing bacterium Nitrosomonas eutropha blocks T helper 2 cell polarization via the anti-inflammatory cytokine IL-10. Sci Rep. 2021;11:14162 pubmed publisher
Chong W, Mattapallil M, Raychaudhuri K, Bing S, Wu S, Zhong Y, et al. The Cytokine IL-17A Limits Th17 Pathogenicity via a Negative Feedback Loop Driven by Autocrine Induction of IL-24. Immunity. 2020;53:384-397.e5 pubmed publisher
Stumpf A, Brandstetter T, Hübner J, Rühe J. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum. PLoS ONE. 2019;14:e0225525 pubmed publisher
Hrdý J, Vlasakova K, Cerny V, Súkeníková L, Novotná O, Petrásková P, et al. Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action. Eur J Immunol. 2018;48:2015-2030 pubmed publisher
Jhun J, Lee S, Lee S, Kim H, Jung E, Kim D, et al. Serial Monitoring of Immune Markers Being Represented Regulatory T Cell/T Helper 17 Cell Ratio: Indicating Tolerance for Tapering Immunosuppression after Liver Transplantation. Front Immunol. 2018;9:352 pubmed publisher
Reuschl A, Edwards M, Parker R, Connell D, Hoang L, Halliday A, et al. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways. PLoS Pathog. 2017;13:e1006577 pubmed publisher
Brencicova E, Jagger A, Evans H, Georgouli M, Laios A, Attard Montalto S, et al. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma. PLoS ONE. 2017;12:e0175712 pubmed publisher
Gonzalez Lombana C, Gimblet C, Bacellar O, Oliveira W, Passos S, Carvalho L, et al. IL-17 mediates immunopathology in the absence of IL-10 following Leishmania major infection. PLoS Pathog. 2013;9:e1003243 pubmed publisher
Raposo R, Trudgian D, Thomas B, van Wilgenburg B, Cowley S, James W. Protein kinase C and NF-?B-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection. J Immunol. 2011;187:748-59 pubmed publisher
Salinthone S, Yadav V, Schillace R, Bourdette D, Carr D. Lipoic acid attenuates inflammation via cAMP and protein kinase A signaling. PLoS ONE. 2010;5: pubmed publisher
Biancotto A, Iglehart S, Lisco A, Vanpouille C, Grivel J, Lurain N, et al. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res Hum Retroviruses. 2008;24:453-62 pubmed publisher
Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
Pfleger C, Schloot N, ter Veld F. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J Immunol Methods. 2008;329:214-8 pubmed
Grivel J, Elliott J, Lisco A, Biancotto A, Condack C, Shattock R, et al. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS. 2007;21:1263-72 pubmed
Biancotto A, Grivel J, Iglehart S, Vanpouille C, Lisco A, Sieg S, et al. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood. 2007;109:4272-9 pubmed
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck L, et al. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin Immunol. 2006;120:220-31 pubmed
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, et al. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology. 2004;112:274-89 pubmed
Copeland S, Siddiqui J, Remick D. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J Immunol Methods. 2004;284:99-106 pubmed

product information

brand :

R&D Systems

master code :

MAB2852

SKU :

MAB2852-100

product name :

Human IFN-gamma Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human IFN-gamma Antibody from R&D Systems is a mouse monoclonal antibody to IFN-gamma. This antibody reacts with human. The Human IFN-gamma Antibody has been validated for the following applications: Array Development,ELISA Development,ELISA Development (Capture),Luminex Development,Flow Cytometry,Neutralization,Functional Assay,ELISA Capture,Western Blot,ELISA Capture (Matched Antibody Pair),Intracellular Staining by Flow Cytometry.

target :

IFN-gamma

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

K3.53

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Neutralization <=2 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

host :

Mouse

immunogen :

E. coli -derived recombinant human IFN-gamma

isotype :

IgG2a

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human

specificity :

Detects human IFN-gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse IFN-gamma , recombinant rat IFN-gamma , recombinant porcine IFN-gamma , recombinant feline IFN-gamma , or recombinant cotton rat IFN-gamma is observed.

gene symbol :

IFNG

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Detection of IFN- gamma antibody in Human PBMCs antibody by Flow Cytometry.

applications :

Western Blot,ELISA Capture (Matched Antibody Pair),Intracellular Staining by Flow Cytometry,Array Development,ELISA Development,ELISA Development (Capture),Luminex Development,Flow Cytometry,Neutralization,Functional Assay,ELISA Capture

USD :

479 USD

alt names :

IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage