TGF-beta 1 Antibody | R&D Systems MAB240-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

TGF-beta 1 Antibody

catalog :

MAB240-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

339 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9016

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, blocking or activating experiments

more info or order :

R&D Systems product webpage

citations: 73

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 3c	Sadallah S, Schmied L, Eken C, Charoudeh H, Amicarella F, Schifferli J. Platelet-Derived Ectosomes Reduce NK Cell Function. J Immunol. 2016;197:1663-71 pubmed publisher
flow cytometry; human; loading ...; fig 4b	Piancone F, Saresella M, Marventano I, La Rosa F, Zoppis M, Agostini S, et al. B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity. Sci Rep. 2016;6:29699 pubmed publisher
	Khan K, Yamamoto K, Fujishita A, Koshiba A, Kuroboshi H, Sakabayashi S, et al. Association between FOXP3+ regulatory T-cells and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts. Reprod Biomed Online. 2019;38:857-869 pubmed publisher
	Lin C, Hsu Y, Huang Y, Shih Y, Wang C, Chiang W, et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med. 2019;11: pubmed publisher
	McMillin M, Grant S, Frampton G, Petrescu A, Williams E, Jefferson B, et al. Elevated circulating TGFβ1 during acute liver failure activates TGFβR2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice. J Neuroinflammation. 2019;16:69 pubmed publisher
	Jiang Y, You F, Zhu J, Zheng C, Yan R, Zeng J. Cryptotanshinone Ameliorates Radiation-Induced Lung Injury in Rats. Evid Based Complement Alternat Med. 2019;2019:1908416 pubmed publisher
	Buscemi L, Price M, Bezzi P, Hirt L. Spatio-temporal overview of neuroinflammation in an experimental mouse stroke model. Sci Rep. 2019;9:507 pubmed publisher
	Yi S, Zhai J, Niu R, Zhu G, Wang M, Liu J, et al. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge. Nat Commun. 2018;9:3879 pubmed publisher
	Ji X, Wang H, Wu Z, Zhong X, Zhu M, Zhang Y, et al. Specific Inhibitor of Smad3 (SIS3) Attenuates Fibrosis, Apoptosis, and Inflammation in Unilateral Ureteral Obstruction Kidneys by Inhibition of Transforming Growth Factor β (TGF-β)/Smad3 Signaling. Med Sci Monit. 2018;24:1633-1641 pubmed
	Wan S, Wang S, Weng L, Zhang G, Lin Z, Fei X, et al. CD8α+CD11c+ Extracellular Vesicles in the Lungs Control Immune Homeostasis of the Respiratory Tract via TGF-β1 and IL-10. J Immunol. 2018;200:1651-1660 pubmed publisher
	Wang G, Zhao F, Yang D, Wang J, Qiu L, Pang X. Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGF?1 and microRNA-34a-5p. Int J Mol Med. 2017;: pubmed publisher
	Shimizu T, Narang N, Chen P, Yu B, Knapp M, Janardanan J, et al. Fibroblast deletion of ROCK2 attenuates cardiac hypertrophy, fibrosis, and diastolic dysfunction. JCI Insight. 2017;2: pubmed publisher
	Park J, Park B, Park K. Suppression of Hepatic Epithelial-to-Mesenchymal Transition by Melittin via Blocking of TGF?/Smad and MAPK-JNK Signaling Pathways. Toxins (Basel). 2017;9: pubmed publisher
	Zhang C, Lim J, Liu J, Ponugoti B, Alsadun S, Tian C, et al. FOXO1 expression in keratinocytes promotes connective tissue healing. Sci Rep. 2017;7:42834 pubmed publisher
	Miroshnychenko O, Chang W, Dragoo J. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration. Am J Sports Med. 2017;45:945-953 pubmed publisher
	Moritz R, LeBaron R, Phelix C, Rupaimoole R, Kim H, Tsin A, et al. Macrophage TGF-β1 and the Proapoptotic Extracellular Matrix Protein BIGH3 Induce Renal Cell Apoptosis in Prediabetic and Diabetic Conditions. Int J Clin Med. 2016;7:496-510 pubmed publisher
	Rebo J, Mehdipour M, Gathwala R, Causey K, Liu Y, Conboy M, et al. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood. Nat Commun. 2016;7:13363 pubmed publisher
	Tian L, Fu P, Zhou M, Gu Y, Li Y, Qi J. Role of urotensin II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells. Int J Mol Med. 2016;38:1831-1838 pubmed publisher
	Tang F, Hansbro P, Burgess J, Ammit A, Baines K, Oliver B. A novel immunomodulatory function of neutrophils on rhinovirus-activated monocytes in vitro. Thorax. 2016;71:1039-1049 pubmed publisher
	Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, et al. Differential TGF? pathway targeting by miR-122 in humans and mice affects liver cancer metastasis. Nat Commun. 2016;7:11012 pubmed publisher
	Bove G, Harris M, Zhao H, Barbe M. Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury. J Neurol Sci. 2016;361:168-80 pubmed publisher
	Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, et al. Regulatory Dendritic Cells Restrain NK Cell IFN-Î³ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. J Immunol. 2015;195:2141-8 pubmed publisher
	Sommer A, Fabri M. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE. 2015;10:e0130395 pubmed publisher
	PÃ¥hlman L, JÃ¶gi A, Gram M, Mori M, Egesten A. Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-Î²1. BMC Pulm Med. 2015;15:19 pubmed publisher
	Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, et al. Dendritic cell SIRT1-HIF1Î± axis programs the differentiation of CD4+ T cells through IL-12 and TGF-Î²1. Proc Natl Acad Sci U S A. 2015;112:E957-65 pubmed publisher
	Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, et al. TGF-Î²3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun. 2015;6:6329 pubmed publisher
	Ogawa H, Ledford J, Mukherjee S, Aono Y, Nishioka Y, Lee J, et al. Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-Î². Respir Res. 2014;15:143 pubmed publisher
	Wiener Z, Band A, Kallio P, Högström J, Hyvönen V, Kaijalainen S, et al. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-β. Proc Natl Acad Sci U S A. 2014;111:E2229-36 pubmed publisher
	Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, et al. Mesenchymal stem cells cancel azoxymethane-induced tumor initiation. Stem Cells. 2014;32:913-25 pubmed publisher
	Qu X, Li X, Zheng Y, Ren Y, Puelles V, Caruana G, et al. Regulation of renal fibrosis by Smad3 Thr388 phosphorylation. Am J Pathol. 2014;184:944-952 pubmed publisher
	Miller M, Petty C, Tompkins M, Fogle J. CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGF? / GARP-mediated mechanism. Virol J. 2014;11:7 pubmed publisher
	Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol. 2014;192:804-16 pubmed publisher
	Bialas A, Stevens B. TGF-? signaling regulates neuronal C1q expression and developmental synaptic refinement. Nat Neurosci. 2013;16:1773-82 pubmed publisher
	Hasegawa T, Yashiro M, Nishii T, Matsuoka J, Fuyuhiro Y, Morisaki T, et al. Cancer-associated fibroblasts might sustain the stemness of scirrhous gastric cancer cells via transforming growth factor-? signaling. Int J Cancer. 2014;134:1785-95 pubmed publisher
	Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, et al. Exosomes with membrane-associated TGF-?1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction. Eur J Immunol. 2013;43:2461-72 pubmed publisher
	Rodrigues T, Matafome P, Santos Silva D, Sena C, Seica R. Reduction of methylglyoxal-induced glycation by pyridoxamine improves adipose tissue microvascular lesions. J Diabetes Res. 2013;2013:690650 pubmed publisher
	Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, et al. A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells. J Immunol. 2013;190:3153-62 pubmed publisher
	Zhu Y, Colak T, Shenoy M, Liu L, Mehta K, Pai R, et al. Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis. Mol Pain. 2012;8:65 pubmed publisher
	Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay M. HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages. PLoS ONE. 2012;7:e32761 pubmed publisher
	Ma W, Han W, Greer P, Tuder R, Toque H, Wang K, et al. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. J Clin Invest. 2011;121:4548-66 pubmed publisher
	Liu F, Chen H, Huang X, Chung A, Zhou L, Fu P, et al. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia. 2011;54:2713-23 pubmed publisher
	Gregory P, Bracken C, Smith E, Bert A, Wright J, Roslan S, et al. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Mol Biol Cell. 2011;22:1686-98 pubmed publisher
	Heise R, Stober V, Cheluvaraju C, Hollingsworth J, Garantziotis S. Mechanical stretch induces epithelial-mesenchymal transition in alveolar epithelia via hyaluronan activation of innate immunity. J Biol Chem. 2011;286:17435-44 pubmed publisher
	Oida T, Weiner H. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß. J Immunol. 2010;185:3529-35 pubmed publisher
	Campillo Gimenez L, Cumont M, Fay M, Kared H, Monceaux V, Diop O, et al. AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection. J Immunol. 2010;184:984-92 pubmed publisher
	Pot C, Jin H, Awasthi A, Liu S, Lai C, Madan R, et al. Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells. J Immunol. 2009;183:797-801 pubmed publisher
	Meltendorf C, Burbach G, Ohrloff C, Ghebremedhin E, Deller T. Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction. Invest Ophthalmol Vis Sci. 2009;50:3688-95 pubmed publisher
	Hosui A, Kimura A, Yamaji D, Zhu B, Na R, Hennighausen L. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation. J Exp Med. 2009;206:819-31 pubmed publisher
	Bhowmick S, Mazumdar T, Ali N. Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection. Infect Immun. 2009;77:1514-23 pubmed publisher
	Cerboni C, Ardolino M, Santoni A, Zingoni A. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood. 2009;113:2955-64 pubmed publisher
	Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, et al. Activation of Toll-like receptor 3 augments myofibroblast differentiation. Am J Respir Cell Mol Biol. 2009;40:654-62 pubmed publisher
	Walsh M, Ampasala D, Hatfield J, Vander Heide R, Suer S, Rishi A, et al. Transforming growth factor-beta stimulates intestinal epithelial focal adhesion kinase synthesis via Smad- and p38-dependent mechanisms. Am J Pathol. 2008;173:385-99 pubmed publisher
	Oh J, Kim M, Shin M, Lee H, Ko J, Wee W, et al. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells. 2008;26:1047-55 pubmed publisher
	Wan N, Dai H, Wang T, Moore Y, Zheng X, Dai Z. Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection. J Immunol. 2008;180:113-21 pubmed
	Acosta Rodriguez E, Napolitani G, Lanzavecchia A, Sallusto F. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat Immunol. 2007;8:942-9 pubmed
	Ball S, Shuttleworth C, Kielty C. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J Cell Biol. 2007;177:489-500 pubmed
	Nazareth M, Broderick L, Simpson Abelson M, Kelleher R, Yokota S, Bankert R. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells. J Immunol. 2007;178:5552-62 pubmed
	Zheng S, Wang J, Wang P, Gray J, Horwitz D. IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. J Immunol. 2007;178:2018-27 pubmed
	Iliopoulou E, Karamouzis M, Missitzis I, Ardavanis A, Sotiriadou N, Baxevanis C, et al. Increased frequency of CD4+ cells expressing CD161 in cancer patients. Clin Cancer Res. 2006;12:6901-9 pubmed
	Rafei M, Wu J, Annabi B, Lejeune L, Francois M, Galipeau J. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Blood. 2007;109:2234-42 pubmed
	Li J, Campanale N, Liang R, Deane J, Bertram J, Ricardo S. Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy. Am J Pathol. 2006;169:1527-40 pubmed
	Ebert E, Mehta V. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell Immunol. 2006;241:7-13 pubmed
	Yang X, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, et al. Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma. J Hepatol. 2006;45:254-62 pubmed
	Cho M, Min S, Chang S, Kim K, Heo S, Lee S, et al. Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression. Immunol Lett. 2006;105:159-66 pubmed
	Mozaffarian D, Rimm E, King I, Lawler R, McDonald G, Levy W. trans fatty acids and systemic inflammation in heart failure. Am J Clin Nutr. 2004;80:1521-5 pubmed
	Lim H, Hillsamer P, Kim C. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J Clin Invest. 2004;114:1640-9 pubmed
	Godfrey W, Spoden D, Ge Y, Baker S, Liu B, Levine B, et al. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood. 2005;105:750-8 pubmed
	Phillips T. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis. 2004;25:1652-9 pubmed
	Jono H, Shuto T, Xu H, Kai H, Lim D, Gum J, et al. Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription. J Biol Chem. 2002;277:45547-57 pubmed
	Schmidt A, Göpfert C, Vlodavsky I, Volker W, Buddecke E. Induction of a hypertrophic growth status of coronary smooth muscle cells is associated with an overexpression of TGF-beta. Eur J Cell Biol. 2002;81:138-44 pubmed
	Liu X, Kohyama T, Wang H, Zhu Y, Wen F, Kim H, et al. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am J Physiol Lung Cell Mol Physiol. 2002;282:L1049-56 pubmed
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product information

SKU :

MAB240-100

product name :

TGF-beta 1 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

Description :

The TGF-beta 1 Antibody from R D Systems is a mouse monoclonal antibody to TGF-beta 1. This antibody reacts with multi-species. The TGF-beta 1 Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready, ELISA Capture (Matched Antibody Pair).

target :

TGF-beta 1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

clonality :

Monoclonal

clone :

9016

conjugate :

Unconjugated

host :

Mouse

immunogen :

Chinese hamster ovary cell line CHO-derived recombinant TGF-beta 1 and latent TGF-beta 1

isotype :

IgG1

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Multi-Species

specificity :

Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human (rh) TGF-beta 3 and recombinant amphibian TGF-beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.

gene symbol :

TGFB1

details of functionality :

ActivityAssaywCitation not found None None None None None None

endotoxin note :

0.10 EU per 1 µg of the antibody by the LAL method.

catalog number base :

MAB240

applications :

Western Blot, Immunohistochemistry, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready, ELISA Capture (Matched Antibody Pair)

2020 Proposedprice USD :

339 USD

alt names :

CEDLAP, DPD1, latency-associated peptide, TGF beta1, TGFB, TGFB1, TGFbeta, TGFbeta 1, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage