Human TIM-3 Antibody | R&D Systems MAB2365 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human TIM-3 Antibody

catalog :

MAB2365

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

344823

reactivity :

human, mouse, rhesus macaque

application :

ELISA, immunocytochemistry, neutralization, flow cytometry

more info or order :

R&D Systems product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 6f	Pinkert J, Boehm H, Trautwein M, Doecke W, Wessel F, Ge Y, et al. T cell-mediated elimination of cancer cells by blocking CEACAM6-CEACAM1 interaction. Oncoimmunology. 2022;11:2008110 pubmed publisher
flow cytometry; human; loading ...; fig s1a	Wang C, Edilova M, Wagar L, Mujib S, Singer M, Bernard N, et al. Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy. J Immunol. 2018;200:558-564 pubmed publisher
flow cytometry; human; loading ...; fig 6a	Cherkassky L, Morello A, Villena Vargas J, Feng Y, Dimitrov D, Jones D, et al. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition. J Clin Invest. 2016;126:3130-44 pubmed publisher
flow cytometry; human; fig s5	Leeansyah E, SvÃ¤rd J, Dias J, Buggert M, NystrÃ¶m J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
flow cytometry; human; fig 4	Japp A, Kursunel M, Meier S, MÃ¤lzer J, Li X, Rahman N, et al. Dysfunction of PSA-specific CD8+ T cells in prostate cancer patients correlates with CD38 and Tim-3 expression. Cancer Immunol Immunother. 2015;64:1487-94 pubmed publisher
flow cytometry; human; fig 3	Schnorfeil F, Lichtenegger F, Emmerig K, Schlueter M, Neitz J, Draenert R, et al. T cells are functionally not impaired in AML: increased PD-1 expression is only seen at time of relapse and correlates with a shift towards the memory T cell compartment. J Hematol Oncol. 2015;8:93 pubmed publisher
flow cytometry; human; loading ...; fig S2,S3	McArthur M, Fresnay S, Magder L, Darton T, Jones C, Waddington C, et al. Activation of Salmonella Typhi-specific regulatory T cells in typhoid disease in a wild-type S. Typhi challenge model. PLoS Pathog. 2015;11:e1004914 pubmed publisher
flow cytometry; human; fig 1	Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
flow cytometry; human ELISA; human	Clayton K, Douglas Vail M, Nur Ur Rahman A, Medcalf K, Xie I, Chew G, et al. Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression. J Virol. 2015;89:3723-36 pubmed publisher
flow cytometry; rhesus macaque	Fujita T, Burwitz B, Chew G, Reed J, Pathak R, Seger E, et al. Expansion of dysfunctional Tim-3-expressing effector memory CD8+ T cells during simian immunodeficiency virus infection in rhesus macaques. J Immunol. 2014;193:5576-83 pubmed publisher
flow cytometry; human	Saresella M, Piancone F, Marventano I, La Rosa F, Tortorella P, Caputo D, et al. A role for the TIM-3/GAL-9/BAT3 pathway in determining the clinical phenotype of multiple sclerosis. FASEB J. 2014;28:5000-9 pubmed publisher
immunocytochemistry; human	Gautron A, Dominguez Villar M, de Marcken M, Hafler D. Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells. Eur J Immunol. 2014;44:2703-2711 pubmed publisher
flow cytometry; human	Gros A, Robbins P, Yao X, Li Y, Turcotte S, Tran E, et al. PD-1 identifies the patient-specific CD8? tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 2014;124:2246-59 pubmed publisher
flow cytometry; human	Hertoghs K, Moerland P, van Stijn A, Remmerswaal E, Yong S, van de Berg P, et al. Molecular profiling of cytomegalovirus-induced human CD8+ T cell differentiation. J Clin Invest. 2010;120:4077-90 pubmed publisher
flow cytometry; human	Vali B, Jones R, Sakhdari A, Sheth P, Clayton K, Yue F, et al. HCV-specific T cells in HCV/HIV co-infection show elevated frequencies of dual Tim-3/PD-1 expression that correlate with liver disease progression. Eur J Immunol. 2010;40:2493-505 pubmed publisher
	Smith M, Chiang C, Zerrouki K, Rahman S, White W, Streicher K, et al. Using the circulating proteome to assess type I interferon activity in systemic lupus erythematosus. Sci Rep. 2020;10:4462 pubmed publisher
	Sun H, Xu J, Huang Q, Huang M, Li K, Qu K, et al. Reduced CD160 Expression Contributes to Impaired NK-cell Function and Poor Clinical Outcomes in Patients with HCC. Cancer Res. 2018;78:6581-6593 pubmed publisher
	Klebanoff C, Crompton J, Leonardi A, Yamamoto T, Chandran S, Eil R, et al. Inhibition of AKT signaling uncouples T cell differentiation from expansion for receptor-engineered adoptive immunotherapy. JCI Insight. 2017;2: pubmed publisher
	Sabins N, Chornoguz O, Leander K, Kaplan F, Carter R, Kinder M, et al. TIM-3 Engagement Promotes Effector Memory T Cell Differentiation of Human Antigen-Specific CD8 T Cells by Activating mTORC1. J Immunol. 2017;199:4091-4102 pubmed publisher
	Stecher C, Battin C, Leitner J, Zettl M, Grabmeier Pfistershammer K, Höller C, et al. PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells. Front Immunol. 2017;8:572 pubmed publisher
	Schwartz J, Clayton K, Mujib S, Zhang H, Rahman A, Liu J, et al. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9. J Immunol. 2017;198:3181-3194 pubmed publisher
	Seifert A, Zeng S, Zhang J, Kim T, Cohen N, Beckman M, et al. PD-1/PD-L1 Blockade Enhances T-cell Activity and Antitumor Efficacy of Imatinib in Gastrointestinal Stromal Tumors. Clin Cancer Res. 2017;23:454-465 pubmed publisher
	Abdiche Y, Yeung Y, Chaparro Riggers J, Barman I, Strop P, Chin S, et al. The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity. MAbs. 2015;7:331-43 pubmed publisher
	Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, et al. Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection. PLoS ONE. 2014;9:e92134 pubmed publisher
	Hansen J, Hanash S, Tabellini L, Baik C, Lawler R, Grogan B, et al. A novel soluble form of Tim-3 associated with severe graft-versus-host disease. Biol Blood Marrow Transplant. 2013;19:1323-30 pubmed publisher
	Golden Mason L, McMahan R, Strong M, Reisdorph R, Mahaffey S, Palmer B, et al. Galectin-9 functionally impairs natural killer cells in humans and mice. J Virol. 2013;87:4835-45 pubmed publisher
	Bi S, Earl L, Jacobs L, Baum L. Structural features of galectin-9 and galectin-1 that determine distinct T cell death pathways. J Biol Chem. 2008;283:12248-58 pubmed publisher

product information

master code :

MAB2365

SKU :

MAB2365

product name :

Human TIM-3 Antibody

unit size :

100 ug (also 25 ug)

description :

The Human TIM-3 Antibody from R&D Systems is a rat monoclonal antibody to TIM-3. This antibody reacts with human,mouse. The Human TIM-3 Antibody has been validated for the following applications: Flow Cytometry,Neutralization,CyTOF-reported.

target :

TIM-3

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

344823

concentration :

LYOPH

conjugate :

Unconjugated

host :

Rat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human TIM-3, Ser22-Arg200, Accession # Q8TDQ0.2

isotype :

IgG2a

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Mouse

specificity :

Detects human TIM-3 in direct ELISAs. Does not cross-react with recombinant human (rh) TIM-1, rhTIM-4, recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-3, rmTIM-5, or rmTIM-6.

gene symbol :

HAVCR2

top caption :

Detection of TIM-3 antibody in Human Monocytes antibody by Flow Cytometry.

accessionNumbers :

Q8TDQ0.2

applications :

Flow Cytometry,Neutralization,CyTOF-reported

extended description :

Clone 344823 was used by HLDA to establish CD designation

USD :

499 USD

product details :

Clone 344823 was used by HLDA to establish CD designation

alt names :

CD366, FLJ14428, HAVcr-2, HAVCR2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, KIM-3, SPTCL, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, Tim-3, TIM3, TIM3 T-cell membrane protein 3, TIMD3, TIMD-3, TIMD3KIM-3

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage