Human IL-12 p70 Antibody | R&D Systems MAB219-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human IL-12 p70 Antibody

catalog :

MAB219-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

479 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

24910

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments

more info or order :

R&D Systems product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 2 ug/ml; loading ...; fig 8a	Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, et al. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience. 2021;24:103433 pubmed publisher
flow cytometry; human; loading ...; fig 2c	Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
	Sinclair J, Vedelago C, Ryan F, Carney M, Redd M, Lynn M, et al. Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function. Nat Microbiol. 2024;9:3135-3147 pubmed publisher
	Serrano R, Coch C, Peters C, Hartmann G, Wesch D, Kabelitz D. Monocyte-dependent co-stimulation of cytokine induction in human γδ T cells by TLR8 RNA ligands. Sci Rep. 2021;11:15231 pubmed publisher
	Fraccarollo D, Neuser J, M xf6 ller J, Riehle C, Galuppo P, Bauersachs J. Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarction. elife. 2021;10: pubmed publisher
	Maura D, Elmekki N, Goddard C. The ammonia oxidizing bacterium Nitrosomonas eutropha blocks T helper 2 cell polarization via the anti-inflammatory cytokine IL-10. Sci Rep. 2021;11:14162 pubmed publisher
	Mata Forsberg M, Arasa C, van Zwol W, Uzunçayir S, Schönbichler A, Regenthal P, et al. Activation of human γδ T cells and NK cells by Staphylococcal enterotoxins requires both monocytes and conventional T cells. J Leukoc Biol. 2021;: pubmed publisher
	Flament H, Rouland M, Beaudoin L, Toubal A, Bertrand L, Lebourgeois S, et al. Outcome of SARS-CoV-2 infection is linked to MAIT cell activation and cytotoxicity. Nat Immunol. 2021;22:322-335 pubmed publisher
	Suliman S, Murphy M, Musvosvi M, Gela A, Meermeier E, Geldenhuys H, et al. MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria. J Immunol. 2019;203:2917-2927 pubmed publisher
	Duarte Neto A, Croda J, Pagliari C, Soriano F, Nicodemo A, Duarte M. Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock. Front Immunol. 2019;10:920 pubmed publisher
	Ricciardi S, Manfrini N, Alfieri R, Calamita P, Crosti M, Gallo S, et al. The Translational Machinery of Human CD4+ T Cells Is Poised for Activation and Controls the Switch from Quiescence to Metabolic Remodeling. Cell Metab. 2018;28:895-906.e5 pubmed publisher
	Porichis F, Hart M, Massa A, Everett H, Morou A, Richard J, et al. Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK Cells. J Immunol. 2018;201:971-981 pubmed publisher
	Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling. Sci Rep. 2016;6:32320 pubmed publisher
	Suliman S, Geldenhuys H, Johnson J, Hughes J, Smit E, Murphy M, et al. Bacillus Calmette-Guérin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses. J Immunol. 2016;197:1100-1110 pubmed publisher
	Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
	Jones R, Mueller S, O Connor R, Rimpel K, Sloan D, Karel D, et al. A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes. PLoS Pathog. 2016;12:e1005545 pubmed publisher
	Ranzani V, Rossetti G, Panzeri I, Arrigoni A, Bonnal R, Curti S, et al. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Nat Immunol. 2015;16:318-325 pubmed publisher
	Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan Garcia A, et al. Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver. PLoS Pathog. 2014;10:e1004210 pubmed publisher
	Blom L, Poulsen L. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures. J Immunol. 2012;189:4331-7 pubmed publisher
	Dubicke A, Fransson E, Centini G, Andersson E, Bystr xf6 m B, Malmstr xf6 m A, et al. Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening. J Reprod Immunol. 2010;84:176-85 pubmed publisher
	Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, et al. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J Immunol. 2009;183:8176-85 pubmed publisher
	Dai Z, Turtle C, Booth G, Riddell S, Gooley T, Stevens A, et al. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus. J Exp Med. 2009;206:793-805 pubmed publisher
	Miller R, Sadhukhan R, Wu C. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay. J Immunol Methods. 2008;334:134-41 pubmed publisher
	Seshasayee D, Lee W, Zhou M, Shu J, Suto E, Zhang J, et al. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation. J Clin Invest. 2007;117:3868-78 pubmed
	Mian M, Lauzon N, Stampfli M, Mossman K, Ashkar A. Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke. J Leukoc Biol. 2008;83:774-84 pubmed
	Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck L, et al. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin Immunol. 2006;120:220-31 pubmed
	Radstake T, Roelofs M, Jenniskens Y, Oppers Walgreen B, van Riel P, Barrera P, et al. Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma. Arthritis Rheum. 2004;50:3856-65 pubmed
	Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, et al. NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs. Blood. 2004;104:3267-75 pubmed
	Weigt H, Muhlradt P, Larbig M, Krug N, Braun A. The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model. J Immunol. 2004;172:6080-6 pubmed
	Durali D, de Goër de Herve M, Giron Michel J, Azzarone B, Delfraissy J, Taoufik Y. In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Blood. 2003;102:4084-9 pubmed

product information

brand :

R&D Systems

master code :

MAB219

SKU :

MAB219-100

product name :

Human IL-12 p70 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human IL-12 p70 Antibody from R&D Systems is a mouse monoclonal antibody to IL-12 p70/IL-12A. This antibody reacts with human,n/a. The Human IL-12 p70 Antibody has been validated for the following applications: Neutralization,Bioassay,Blocking,Flow Cytometry,ELISA Capture,Immunohistochemistry-Paraffin,Immunocytochemistry,Immunohistochemistry,Western Blot.

target :

IL-12 p70

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

24910

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Immunohistochemistry 8-25 ug/mL, Neutralization 0.3-0.9 ug/mL

host :

Mouse

immunogen :

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p70

isotype :

IgG1

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,N/A

specificity :

Detects human IL-12 p70 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-12 p40 homodimer or rhIL-23 is observed.

gene symbol :

IL12A

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

IL-12 p70 antibody in Human Kidney by Immunohistochemistry (IHC-P).

applications :

Immunohistochemistry,Western Blot,Bioassay,Blocking,Flow Cytometry,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,ELISA Capture

USD :

479 USD

alt names :

CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage