product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse CXCR2/IL-8RB Antibody
catalog :
MAB2164-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
489 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
242216
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 46
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; mouse; ; fig 6h
Pavlidis P, Tsakmaki A, Pantazi E, Li K, Cozzetto D, Digby Bell J, et al. Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy. Nat Commun. 2022;13:5820 pubmed publisher
Xue P, Lv L, Liu L, Xu Y, Zhou C, Wang Y. Unveiling the role of CXCL8/CXCR2 in intervertebral disc degeneration: A path to promising therapeutic strategies. J Orthop Translat. 2024;49:119-134 pubmed publisher
Wu F, Han Y, Xiong Q, Tang H, Shi J, Yang Q, et al. Cerebral Endothelial CXCR2 Promotes Neutrophil Transmigration into Central Nervous System in LPS-Induced Septic Encephalopathy. Biomedicines. 2024;12: pubmed publisher
Sawant K, Sepuru K, Penaranda B, Lowry E, Garofalo R, Rajarathnam K. Chemokine Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant. J Leukoc Biol. 2023;114:666-671 pubmed publisher
Luo S, Liao C, Zhang L, Ling C, Zhang X, Xie P, et al. METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling. Cell Rep. 2023;42:112259 pubmed publisher
Shimada A, Hasegawa Ishii S. Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice. Toxicol Rep. 2021;8:520-528 pubmed publisher
Galeano Niño J, Pageon S, Tay S, Colakoglu F, Kempe D, Hywood J, et al. Cytotoxic T cells swarm by homotypic chemokine signalling. elife. 2020;9: pubmed publisher
Ogawa K, Asano K, Yotsumoto S, Yamane T, Arita M, Hayashi Y, et al. Frontline Science: Conversion of neutrophils into atypical Ly6G+ SiglecF+ immune cells with neurosupportive potential in olfactory neuroepithelium. J Leukoc Biol. 2021;109:481-496 pubmed publisher
Aquino R, Hayashida A, Park P. Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps. PLoS Pathog. 2020;16:e1008497 pubmed publisher
Zhang Z, Dong L, Jia A, Chen X, Yang Q, Wang Y, et al. Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells. Cancers (Basel). 2020;12: pubmed publisher
Park M, Kim H, Lee H, Zabel B, Bae Y. Novel CD11b+Gr-1+Sca-1+ myeloid cells drive mortality in bacterial infection. Sci Adv. 2020;6:eaax8820 pubmed publisher
Mohammad M, Nguyen M, Engdahl C, Na M, Jarneborn A, Hu Z, et al. The YIN and YANG of lipoproteins in developing and preventing infectious arthritis by Staphylococcus aureus. PLoS Pathog. 2019;15:e1007877 pubmed publisher
Capitano M, Mor Vaknin N, Saha A, Cooper S, Legendre M, Guo H, et al. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling. J Clin Invest. 2019;129:2555-2570 pubmed publisher
Kozasa K, Mabuchi S, Matsumoto Y, Kuroda H, Yokoi E, Komura N, et al. Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models. Oncotarget. 2019;10:1887-1902 pubmed publisher
Liao W, Overman M, Boutin A, Shang X, Zhao D, Dey P, et al. KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer. Cancer Cell. 2019;35:559-572.e7 pubmed publisher
Girbl T, Lenn T, Pérez L, Rolas L, Barkaway A, Thiriot A, et al. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis. Immunity. 2018;49:1062-1076.e6 pubmed publisher
Mollaoglu G, Jones A, Wait S, Mukhopadhyay A, Jeong S, Arya R, et al. The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 2018;49:764-779.e9 pubmed publisher
Vikhe P, Purnell T, Brown S, Hood D. Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear. Cell Microbiol. 2019;21:e12960 pubmed publisher
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
Watanabe K, Gilchrist C, Uddin M, Burgess S, Abhyankar M, Moonah S, et al. Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis. PLoS Pathog. 2017;13:e1006513 pubmed publisher
Zhang C, Wang S, Li J, Zhang W, Zheng L, Yang C, et al. The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury. Cell Death Dis. 2017;8:e2695 pubmed publisher
Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, et al. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. J Biol Chem. 2015;290:2053-68 pubmed publisher
Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, et al. The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation. Mol Cell Biol. 2015;35:598-609 pubmed publisher
Labelle M, Begum S, Hynes R. Platelets guide the formation of early metastatic niches. Proc Natl Acad Sci U S A. 2014;111:E3053-61 pubmed publisher
Highfill S, Cui Y, Giles A, Smith J, Zhang H, Morse E, et al. Disruption of CXCR2-mediated MDSC tumor trafficking enhances anti-PD1 efficacy. Sci Transl Med. 2014;6:237ra67 pubmed publisher
Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, et al. Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2. EMBO Mol Med. 2014;6:810-20 pubmed publisher
Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, et al. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments. Exp Cell Res. 2014;323:7-27 pubmed publisher
Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, et al. mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury. J Leukoc Biol. 2014;95:961-70 pubmed publisher
Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, et al. Phosphatase Wip1 negatively regulates neutrophil migration and inflammation. J Immunol. 2014;192:1184-95 pubmed publisher
Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, et al. IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-?-activated macrophage programming. J Immunol. 2014;192:704-13 pubmed publisher
Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-?. Int J Cancer. 2014;134:1346-58 pubmed publisher
Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, et al. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol. 2013;190:5329-36 pubmed publisher
Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, et al. Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection. Eur J Immunol. 2013;43:1322-32 pubmed publisher
Roy M, Richard J, Dumas A, Vallières L. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. J Neuroinflammation. 2012;9:18 pubmed publisher
Halpern J, Kilbarger A, Lynch C. Mesenchymal stem cells promote mammary cancer cell migration in vitro via the CXCR2 receptor. Cancer Lett. 2011;308:91-9 pubmed publisher
Zhao C, Sardella A, Chun J, Poubelle P, Fernandes M, Bourgoin S. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines. J Lipid Res. 2011;52:1307-18 pubmed publisher
von Vietinghoff S, Asagiri M, Azar D, Hoffmann A, Ley K. Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice. J Immunol. 2010;185:670-8 pubmed publisher
Traylor Z, Yu E, Davis I. Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice. Am J Physiol Lung Cell Mol Physiol. 2010;298:L437-45 pubmed publisher
Lemos H, Grespan R, Vieira S, Cunha T, Verri W, Fernandes K, et al. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc Natl Acad Sci U S A. 2009;106:5954-9 pubmed publisher
Omari K, Lutz S, Santambrogio L, Lira S, Raine C. Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1. Am J Pathol. 2009;174:164-76 pubmed publisher
Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge J, Abe J, et al. Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice. Blood. 2008;111:5457-66 pubmed publisher
Nomellini V, Faunce D, Gomez C, Kovacs E. An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition. J Leukoc Biol. 2008;83:1493-501 pubmed publisher
Scortegagna M, Cataisson C, Martin R, Hicklin D, Schreiber R, Yuspa S, et al. HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling. Blood. 2008;111:3343-54 pubmed publisher
Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice. Eur J Immunol. 2007;37:2753-63 pubmed
Mohsenin A, Mi T, Xia Y, Kellems R, Chen J, Blackburn M. Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice. Am J Physiol Lung Cell Mol Physiol. 2007;293:L753-61 pubmed
Yu J, Ruddy M, Wong G, Sfintescu C, Baker P, Smith J, et al. An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals. Blood. 2007;109:3794-802 pubmed
product information
brand :
R&D Systems
master code :
MAB2164
SKU :
MAB2164-100
product name :
Mouse CXCR2/IL-8RB Antibody
unit size :
100 ug (also 25 ug, 500 ug)
seo description :
The Mouse CXCR2/IL-8RB Antibody from R&D Systems is a rat monoclonal antibody to CXCR2/IL-8RB. This antibody reacts with human,mouse,rat,transgenic mouse. The Mouse CXCR2/IL-8RB Antibody has been validated for the following applications: Bioassay,Flow Cytometry,In vivo assay,Immunohistochemistry,Western Blot,Immunocytochemistry,Neutralization,Functional Assay,Immunohistochemistry-Frozen,CyTOF-reported.
target :
CXCR2/IL-8RB
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
242216
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization 15-50 ug/mL, CyTOF-reported
host :
Rat
immunogen :
C6 rat glioma cell line transfected with mouse CXCR2, Met1-Leu359, Accession # P35343
isotype :
IgG2a
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,Rat,Transgenic Mouse
specificity :
Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.
gene symbol :
CXCR2
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of CXCR2/IL-8 RB antibody in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry.
accessionNumbers :
P35343
applications :
CyTOF-reported,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunocytochemistry,Functional Assay
USD :
489 USD
alt names :
CD182, CD182 antigen, CDw128b, chemokine (C-X-C motif) receptor 2, chemokine (CXC) receptor 2, CMKAR2, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CXCR2 gene for IL8 receptor type B, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8 RB, IL-8 receptor type 2, IL-8R B, IL8R2, IL8RA, IL8RB, interleukin 8 receptor B, interleukin 8 receptor type 2, interleukin-8 receptor type B
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.