product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse/Rat Vimentin Antibody
catalog :
MAB2105
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
280618
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:500; loading ...; fig s3e
O Shea T, Ao Y, Wang S, Wollenberg A, Kim J, Ramos Espinoza R, et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice. Nat Commun. 2022;13:5702 pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...; fig s7i
Cui M, Atmanli A, Morales M, Tan W, Chen K, Xiao X, et al. Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance. Nat Commun. 2021;12:5270 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1f
Sarvestani S, SIGNS S, Hu B, Yeu Y, Feng H, Ni Y, et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun. 2021;12:262 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2c
Lauver M, Goetschius D, Netherby Winslow C, Ayers K, Jin G, Haas D, et al. Antibody escape by polyomavirus capsid mutation facilitates neurovirulence. elife. 2020;9: pubmed publisher
  • immunohistochemistry; human; loading ...; fig 3a
Szvicsek Z, Oszvald Á, Szabó L, Sándor G, Kelemen A, Soós A, et al. Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors. Cell Mol Life Sci. 2019;76:2463-2476 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 2d,2e
Grzelak C, Sigglekow N, Tirnitz Parker J, Hamson E, Warren A, Maneck B, et al. Widespread GLI expression but limited canonical hedgehog signaling restricted to the ductular reaction in human chronic liver disease. PLoS ONE. 2017;12:e0171480 pubmed publisher
  • immunohistochemistry; rat; 1:100; fig 2
E L, Xu W, Feng L, Liu Y, Cai D, Wen N, et al. Estrogen enhances the bone regeneration potential of periodontal ligament stem cells derived from osteoporotic rats and seeded on nano-hydroxyapatite/collagen/poly(L-lactide). Int J Mol Med. 2016;37:1475-86 pubmed publisher
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 2
Forni M, Ramos Maia Lobba A, Pereira Ferreira A, Sogayar M. Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin. PLoS ONE. 2015;10:e0140143 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 1
Ko J, Klimowicz A, Jagdis A, Phan T, Laskin J, Lau H, et al. ATM, THMS, and RRM1 protein expression in nasopharyngeal carcinomas treated with curative intent. Head Neck. 2016;38 Suppl 1:E384-91 pubmed publisher
Prondzynski M, Berkson P, Trembley M, Tharani Y, Shani K, Bortolin R, et al. Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems. Nat Commun. 2024;15:5929 pubmed publisher
Song X, Nihashi Y, Imai Y, Mori N, Kagaya N, Suenaga H, et al. Collagen Lattice Model, Populated with Heterogeneous Cancer-Associated Fibroblasts, Facilitates Advanced Reconstruction of Pancreatic Cancer Microenvironment. Int J Mol Sci. 2024;25: pubmed publisher
Abubaker M, Greaney A, Newport D, Mulvihill J. Characterization of primary human leptomeningeal cells in 2D culture. Heliyon. 2024;10:e26744 pubmed publisher
L xf3 pez Guajardo A, Zafar A, Al Hennawi K, Rossi V, Alrwaili A, Medcalf J, et al. Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6. Front Mol Biosci. 2023;10:1197814 pubmed publisher
Shimizu K, Kikuta J, Ohta Y, Uchida Y, Miyamoto Y, Morimoto A, et al. Single-cell transcriptomics of human cholesteatoma identifies an activin A-producing osteoclastogenic fibroblast subset inducing bone destruction. Nat Commun. 2023;14:4417 pubmed publisher
Park G, Lim J, Choi E, Lim M, Yun B, Kim D, et al. Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia. Biomater Res. 2023;27:70 pubmed publisher
Shiju T, Sampaio L, Hilgert G, Wilson S. Corneal epithelial basement membrane assembly is mediated by epithelial cells in coordination with corneal fibroblasts during wound healing. Mol Vis. 2023;29:68-86 pubmed
Singh D, Carcamo S, Farias E, Hasson D, Zheng W, Sun D, et al. 5-Azacytidine- and retinoic-acid-induced reprogramming of DCCs into dormancy suppresses metastasis via restored TGF-β-SMAD4 signaling. Cell Rep. 2023;42:112560 pubmed publisher
Polisetti N, Reinhard T, Schlunck G. Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering. Int J Mol Sci. 2023;24: pubmed publisher
Kim H, Warrington S, L xf3 pez Guajardo A, Al Hennawi K, Cook S, Griffith Z, et al. ALD-R491 regulates vimentin filament stability and solubility, cell contractile force, cell migration speed and directionality. Front Cell Dev Biol. 2022;10:926283 pubmed publisher
Lauver M, Jin G, Ayers K, Carey S, Specht C, Abendroth C, et al. T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus. elife. 2022;11: pubmed publisher
Chimienti R, Baccega T, Torchio S, Manenti F, Pellegrini S, Cospito A, et al. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for β cell replacement. Cell Rep. 2022;40:111423 pubmed publisher
Rodriguez Tirado C, Kale N, Carlini M, Shrivastava N, Rodrigues A, Khalil B, et al. NR2F1 Is a Barrier to Dissemination of Early-Stage Breast Cancer Cells. Cancer Res. 2022;82:2313-2326 pubmed publisher
Miyazaki Y, Mori N, Akagi Y, Oda T, Kida Y. Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts. Cancers (Basel). 2022;14: pubmed publisher
Capeling M, Huang S, Childs C, Wu J, Tsai Y, Wu A, et al. Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep. 2022;38:110379 pubmed publisher
de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O Shea E. The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease. elife. 2021;10: pubmed publisher
Coquand L, Victoria G, Tata A, Carpentieri J, Brault J, Guimiot F, et al. CAMSAPs organize an acentrosomal microtubule network from basal varicosities in radial glial cells. J Cell Biol. 2021;220: pubmed publisher
Park G, Yoon J, Yoo S, Song Y, Song P, Kim H, et al. Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma. Mar Drugs. 2021;19: pubmed publisher
Tashireva L, Savelieva O, Grigoryeva E, Nikitin Y, Denisov E, Vtorushin S, et al. Heterogeneous Manifestations of Epithelial-Mesenchymal Plasticity of Circulating Tumor Cells in Breast Cancer Patients. Int J Mol Sci. 2021;22: pubmed publisher
Yamakawa D, Katoh D, Kasahara K, Shiromizu T, Matsuyama M, Matsuda C, et al. Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis. Cell Rep. 2021;34:108817 pubmed publisher
Li K, Zhou Z, Li J, Xiang R. miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1. Technol Cancer Res Treat. 2020;19:1533033820959404 pubmed publisher
Wang Z, Cui M, Shah A, Tan W, Liu N, Bassel Duby R, et al. Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution. Cell Rep. 2020;33:108472 pubmed publisher
Saikia P, Crabb J, Dibbin L, Juszczak M, Willard B, Jang G, et al. Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea. Sci Rep. 2020;10:16717 pubmed publisher
Bordignon P, Bottoni G, Xu X, Popescu A, Truan Z, Guenova E, et al. Dualism of FGF and TGF-β Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant. Cell Rep. 2019;28:2358-2372.e6 pubmed publisher
Lopresti A, Malergue F, Bertucci F, Liberatoscioli M, Garnier S, DaCosta Q, et al. Sensitive and easy screening for circulating tumor cells by flow cytometry. JCI Insight. 2019;5: pubmed publisher
Wichaiyo S, Lax S, Montague S, Li Z, Grygielska B, Pike J, et al. Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice. Haematologica. 2019;: pubmed publisher
Liu P, Shah R, Li Y, Arora A, Ung P, Raman R, et al. An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy. Nat Cell Biol. 2019;21:203-213 pubmed publisher
Langer E, Allen Petersen B, King S, Kendsersky N, Turnidge M, Kuziel G, et al. Modeling Tumor Phenotypes In Vitro with Three-Dimensional Bioprinting. Cell Rep. 2019;26:608-623.e6 pubmed publisher
Pietraszkiewicz A, Hampton C, Caplash S, Lei L, Capetanaki Y, Tadvalkar G, et al. Desmin deficiency is not sufficient to prevent corneal fibrosis. Exp Eye Res. 2019;180:155-163 pubmed publisher
Cajigas Du Ross C, Martinez S, Woods Burnham L, Durán A, Roy S, Basu A, et al. RNA sequencing reveals upregulation of a transcriptomic program associated with stemness in metastatic prostate cancer cells selected for taxane resistance. Oncotarget. 2018;9:30363-30384 pubmed publisher
Kane J, Magliocco A, Zhang Q, Wang D, Klimowicz A, Harris J, et al. Correlation of High-Risk Soft Tissue Sarcoma Biomarker Expression Patterns with Outcome following Neoadjuvant Chemoradiation. Sarcoma. 2018;2018:8310950 pubmed publisher
Verheecke M, Hermans E, Tuyaerts S, Souche E, van Bree R, Verbist G, et al. Acute Drug Effects on the Human Placental Tissue: The Development of a Placental Murine Xenograft Model. Reprod Sci. 2018;25:1637-1648 pubmed publisher
Ye Q, Lei L, Shao L, Shi J, Jia J, Tong X. MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion. Mol Med Rep. 2017;16:6743-6749 pubmed publisher
Procopio M, László C, Al Labban D, Kim D, Bordignon P, Jo S, et al. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation. Nat Cell Biol. 2015;17:1193-204 pubmed publisher
da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M. The TNF Family Molecules LIGHT and Lymphotoxin αβ Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells. J Immunol. 2015;195:2429-41 pubmed publisher
Riess J, West R, Dean M, Klimowicz A, Neal J, Hoang C, et al. GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray. Anticancer Res. 2015;35:669-76 pubmed
Hao D, Phan T, Jagdis A, Siever J, Klimowicz A, Laskin J, et al. Evaluation of E-cadherin, β-catenin and vimentin protein expression using quantitative immunohistochemistry in nasopharyngeal carcinoma patients. Clin Invest Med. 2014;37:E320-30 pubmed
Peng S, Zhang J, Chen J, Wang H. Effects of Wnt5a protein on proliferation and apoptosis in JAR choriocarcinoma cells. Mol Med Rep. 2011;4:99-104 pubmed publisher
Vrana N, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, et al. Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Invest Ophthalmol Vis Sci. 2008;49:5325-31 pubmed publisher
product information
master code :
MAB2105
SKU :
MAB2105
product name :
Human/Mouse/Rat Vimentin Antibody
unit size :
100 ug (also 25 ug)
description :
The Human/Mouse/Rat Vimentin Antibody from R&D Systems is a rat monoclonal antibody to Vimentin. This antibody reacts with human,mouse,rabbit,rat,transgenic mouse,xenograft. The Human/Mouse/Rat Vimentin Antibody has been validated for the following applications: Immunocytochemistry,Western Blot,Immunohistochemistry,Multiplex Immunofluorescence,Flow Cytometry,Immunohistochemistry-Paraffin,Immunofluorescence,Simple Western,Intracellular Staining by Flow Cytometry,Knockout Validated.
target :
Vimentin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
280618
concentration :
LYOPH
conjugate :
Unconjugated
host :
Rat
immunogen :
E. coli -derived recombinant human Vimentin, Ser2-Glu466, Accession # P08670
isotype :
IgG2a
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,Rabbit,Rat,Xenograft
specificity :
Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots.
gene symbol :
VIM
Antibody validation :
Knockout/Knockdown
accessionNumbers :
P08670
applications :
Western Blot,Immunohistochemistry,Immunocytochemistry,Multiplex Immunofluorescence,Flow Cytometry,Immunohistochemistry-Paraffin,Immunofluorescence,Simple Western,Intracellular Staining by Flow Cytometry,Knockout Validated
USD :
489 USD
alt names :
epididymis secretory sperm binding protein, FLJ36605, VIM, vimentin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.