This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
antibody
product name :
Human/Primate IL-6 Antibody
catalog :
MAB206
quantity :
100 ug (also 25 ug, 500 ug)
price :
369 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6708
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, neutralization, other
citations: 66
Published Application/Species/Sample/DilutionReference
  • ELISA; human; loading ...; tbl s7
Bouhaddou M, Memon D, Meyer B, White K, Rezelj V, Correa Marrero M, et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell. 2020;182:685-712.e19 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6g
Zhu C, Chen X, Guan G, Zou C, Guo Q, Cheng P, et al. IFI30 Is a Novel Immune-Related Target with Predicting Value of Prognosis and Treatment Response in Glioblastoma. Onco Targets Ther. 2020;13:1129-1143 pubmed publisher
  • other; human; loading ...; fig s1
Shah F, Stepan A, O Mahony A, Velichko S, Folias A, Houle C, et al. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators. Cell Chem Biol. 2017;24:858-869.e5 pubmed publisher
Hook J, Cao M, Weng K, Kinnare N, Moreland J. Mycobacterium tuberculosis Lipoarabinomannan Activates Human Neutrophils via a TLR2/1 Mechanism Distinct from Pam3CSK4. J Immunol. 2020;204:671-681 pubmed publisher
Sun X, Qu Q, Lao Y, Zhang M, Yin X, Zhu H, et al. Tumor suppressor HIC1 is synergistically compromised by cancer-associated fibroblasts and tumor cells through the IL-6/pSTAT3 axis in breast cancer. BMC Cancer. 2019;19:1180 pubmed publisher
Stumpf A, Brandstetter T, Hübner J, Rühe J. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum. PLoS ONE. 2019;14:e0225525 pubmed publisher
Hooda J, Novak M, Salomon M, Matsuba C, Ramos R, MacDuffie E, et al. Early Loss of Histone H2B Monoubiquitylation Alters Chromatin Accessibility and Activates Key Immune Pathways That Facilitate Progression of Ovarian Cancer. Cancer Res. 2019;79:760-772 pubmed publisher
Kuranda K, Jean Alphonse P, Leborgne C, Hardet R, Collaud F, Marmier S, et al. Exposure to wild-type AAV drives distinct capsid immunity profiles in humans. J Clin Invest. 2018;128:5267-5279 pubmed publisher
Logue S, McGrath E, Cleary P, Greene S, Mnich K, Almanza A, et al. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy. Nat Commun. 2018;9:3267 pubmed publisher
Rolvering C, Zimmer A, Ginolhac A, Margue C, Kirchmeyer M, Servais F, et al. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies. J Leukoc Biol. 2018;104:969-985 pubmed publisher
Gril B, Paranjape A, Woditschka S, Hua E, Dolan E, Hanson J, et al. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases. Nat Commun. 2018;9:2705 pubmed publisher
Micera A, Di Zazzo A, Esposito G, Longo R, Foulsham W, Sacco R, et al. Age-Related Changes to Human Tear Composition. Invest Ophthalmol Vis Sci. 2018;59:2024-2031 pubmed publisher
Touil H, Kobert A, Lebeurrier N, Rieger A, Saikali P, Lambert C, et al. Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis. J Neuroinflammation. 2018;15:114 pubmed publisher
Sliwinska Mosson M, Milnerowicz S, Milnerowicz H. Diabetes mellitus secondary to pancreatic diseases (type 3c): The effect of smoking on the exocrine-endocrine interactions of the pancreas. Diab Vasc Dis Res. 2018;15:243-259 pubmed publisher
Galland S, Vuille J, Martin P, Letovanec I, Caignard A, Fregni G, et al. Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets. Cell Rep. 2017;20:2891-2905 pubmed publisher
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3. Cell Physiol Biochem. 2017;42:1961-1972 pubmed publisher
Wang D, Tang S, Zhang Q. Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway. Oncol Lett. 2017;13:4875-4881 pubmed publisher
Raimbourg J, Joalland M, Cabart M, de Plater L, Bouquet F, Savina A, et al. Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib. Mol Cancer Ther. 2017;16:1634-1644 pubmed publisher
Zhou J, Zhang C, Pan J, Chen L, Qi S. Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration. Mol Med Rep. 2017;15:4123-4131 pubmed publisher
Ortiz Montero P, Londono Vallejo A, Vernot J. Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line. Cell Commun Signal. 2017;15:17 pubmed publisher
Brencicova E, Jagger A, Evans H, Georgouli M, Laios A, Attard Montalto S, et al. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma. PLoS ONE. 2017;12:e0175712 pubmed publisher
Munir H, Ward L, Sheriff L, Kemble S, Nayar S, Barone F, et al. Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner. Stem Cells. 2017;35:1636-1646 pubmed publisher
Chehboun S, Labrecque Carbonneau J, Pasquin S, Meliani Y, Meddah B, Ferlin W, et al. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling. J Biol Chem. 2017;292:6644-6656 pubmed publisher
Henry C, Martin S. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation. Mol Cell. 2017;65:715-729.e5 pubmed publisher
Chimen M, Yates C, McGettrick H, Ward L, Harrison M, Apta B, et al. Monocyte Subsets Coregulate Inflammatory Responses by Integrated Signaling through TNF and IL-6 at the Endothelial Cell Interface. J Immunol. 2017;198:2834-2843 pubmed publisher
Hsia H, Hutti J, Baldwin A. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding Kinase 1 (TBK1) to Restrain STAT3 Activity. J Biol Chem. 2017;292:5405-5417 pubmed publisher
Nandana S, Tripathi M, Duan P, Chu C, Mishra R, Liu C, et al. Bone Metastasis of Prostate Cancer Can Be Therapeutically Targeted at the TBX2-WNT Signaling Axis. Cancer Res. 2017;77:1331-1344 pubmed publisher
Uchibori T, Matsuda K, Shimodaira T, Sugano M, Uehara T, Honda T. IL-6 trans-signaling is another pathway to upregulate Osteopontin. Cytokine. 2017;90:88-95 pubmed publisher
Munir H, Luu N, Clarke L, Nash G, McGettrick H. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium. PLoS ONE. 2016;11:e0155161 pubmed publisher
Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, et al. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis. J Biol Chem. 2015;290:22212-24 pubmed publisher
Pathak J, Bakker A, Verschueren P, Lems W, Luyten F, Klein Nulend J, et al. CXCL8 and CCL20 Enhance Osteoclastogenesis via Modulation of Cytokine Production by Human Primary Osteoblasts. PLoS ONE. 2015;10:e0131041 pubmed publisher
Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, et al. Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer. Cancer Res. 2015;75:2629-40 pubmed publisher
Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, et al. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci. 2015;35:2452-64 pubmed publisher
Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, et al. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition. Endocrinology. 2015;156:670-9 pubmed publisher
Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, et al. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk. J Immunol. 2014;193:234-43 pubmed publisher
Liu W, Lin Y, Xiao H, Xing S, Chen H, Chi P, et al. Epstein-Barr virus infection induces indoleamine 2,3-dioxygenase expression in human monocyte-derived macrophages through p38/mitogen-activated protein kinase and NF-κB pathways: impairment in T cell functions. J Virol. 2014;88:6660-71 pubmed publisher
Mok J, Mindrinos M, Davis R, Javanmard M. Digital microfluidic assay for protein detection. Proc Natl Acad Sci U S A. 2014;111:2110-5 pubmed publisher
Todd J, Simpson P, Estis J, Torres V, Wub A. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine. 2013;64:660-5 pubmed publisher
Mace T, Ameen Z, Collins A, Wojcik S, Mair M, Young G, et al. Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner. Cancer Res. 2013;73:3007-18 pubmed publisher
Nicolaidou V, Wong M, Redpath A, Ersek A, Baban D, Williams L, et al. Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation. PLoS ONE. 2012;7:e39871 pubmed publisher
Zhao X, Qureshi F, Eastman P, Manning W, Alexander C, Robinson W, et al. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J Immunol Methods. 2012;378:72-80 pubmed publisher
Hong Y, Moon S, Joo Y, Jeon C, Cho M, Ju J, et al. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. J Korean Med Sci. 2011;26:1132-9 pubmed publisher
Zepp J, Nold Petry C, Dinarello C, Nold M. Protection from RNA and DNA viruses by IL-32. J Immunol. 2011;186:4110-8 pubmed publisher
Tso G, Law H, Tu W, Chan G, Lau Y. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Stem Cells. 2010;28:939-54 pubmed publisher
Wu W, Mehta H, Chakrabarty K, Booth J, Duggan E, Patel K, et al. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J Immunol. 2009;183:5799-806 pubmed publisher
Kurago Z, Lam ubol A, Stetsenko A, De La Mater C, Chen Y, Dawson D. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation. Head Neck Pathol. 2008;2:1-12 pubmed publisher
Hu H, Kwun J, Aizenstein B, Knechtle S. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation. 2009;87:1814-20 pubmed publisher
Aliahmadi E, Gramlich R, Grützkau A, Hitzler M, Krüger M, Baumgrass R, et al. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Eur J Immunol. 2009;39:1221-30 pubmed publisher
Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, et al. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. J Immunol. 2008;181:2772-80 pubmed
Osei Kumah A, Smith R, Clifton V. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Cytokine. 2008;43:187-93 pubmed publisher
Nold M, Nold Petry C, Pott G, Zepp J, Saavedra M, Kim S, et al. Endogenous IL-32 controls cytokine and HIV-1 production. J Immunol. 2008;181:557-65 pubmed
Kambas K, Markiewski M, Pneumatikos I, Rafail S, Theodorou V, Konstantonis D, et al. C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome. J Immunol. 2008;180:7368-75 pubmed
Mitchell C, Balkus J, Agnew K, Cohn S, Luque A, Lawler R, et al. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines. AIDS Res Hum Retroviruses. 2008;24:667-71 pubmed publisher
Liotta F, Angeli R, Cosmi L, Filì L, Manuelli C, Frosali F, et al. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Stem Cells. 2008;26:279-89 pubmed
Harrison M, Obermueller E, Maisey N, Hoare S, Edmonds K, Li N, et al. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. J Clin Oncol. 2007;25:4542-9 pubmed
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
Arnold R, Neumann M, König W. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. Immunology. 2007;121:71-81 pubmed
Filer A, Parsonage G, Smith E, Osborne C, Thomas A, Curnow S, et al. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum. 2006;54:2096-108 pubmed
Pavon L, Sandoval López G, Eugenia Hernández M, Loría F, Estrada I, Perez M, et al. Th2 cytokine response in Major Depressive Disorder patients before treatment. J Neuroimmunol. 2006;172:156-65 pubmed
Holst R, Mattsby Baltzer I, Wennerholm U, Hagberg H, Jacobsson B. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery. Acta Obstet Gynecol Scand. 2005;84:551-7 pubmed
Mozaffarian D, Rimm E, King I, Lawler R, McDonald G, Levy W. trans fatty acids and systemic inflammation in heart failure. Am J Clin Nutr. 2004;80:1521-5 pubmed
Phillips T. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis. 2004;25:1652-9 pubmed
Copeland S, Siddiqui J, Remick D. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J Immunol Methods. 2004;284:99-106 pubmed
McLoughlin R, Witowski J, Robson R, Wilkinson T, Hurst S, Williams A, et al. Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation. J Clin Invest. 2003;112:598-607 pubmed
Hurst S, McLoughlin R, Monslow J, Owens S, Morgan L, Fuller G, et al. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J Immunol. 2002;169:5244-51 pubmed
Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, et al. The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes. Arthritis Rheum. 2001;44:2392-401 pubmed
product information
brand :
R&D Systems
master code :
MAB206
SKU :
MAB206-100
product name :
Human/Primate IL-6 Antibody
description :
The Human/Primate IL-6 Antibody from R&D Systems is a mouse monoclonal antibody to IL-6. This antibody reacts with human, primate. The Human/Primate IL-6 Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Neutralization, ELISA Capture (Matched Antibody Pair).
target :
IL-6
category :
Primary Antibodies
unit size :
100 ug (also 25 ug, 500 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
clonality :
Monoclonal
clone :
6708
concentration :
LYOPH
conjugate :
Unconjugated
host :
Mouse
immunogen :
E. coli -derived recombinant human IL-6
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human, Primate
specificity :
Detects human and primate IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL-6, rhOSM, rhLIF, rhIL-11, rhgp130, or rhCNTF.
gene symbol :
IL6
endotoxin note :
<0.10 EU per 1 µg of the antibody by the LAL method.
catalog number base :
MAB206
applications :
Western Blot, Immunohistochemistry, Neutralization, ELISA Capture (Matched Antibody Pair)
2020 USD :
339
2021 USD :
369 USD
alt names :
B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HGF, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.