IL-1 beta/IL-1F2 Antibody | R&D Systems MAB201-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

IL-1 beta/IL-1F2 Antibody

catalog :

MAB201-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

453 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8516

reactivity :

human, rat

application :

western blot, immunocytochemistry, neutralization

more info or order :

R&D Systems product webpage

citations: 33

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; loading ...; fig 4a	Li Y, Li Y, Li L, Yin M, Wang J, Li X. PKR deficiency alleviates pulmonary hypertension via inducing inflammasome adaptor ASC inactivation. Pulm Circ. 2021;11:20458940211046156 pubmed publisher
	Li Y, Zhu Y, Feng S, Ishida Y, Chiu T, Saito T, et al. Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3. Cell Rep. 2022;38:110284 pubmed publisher
	Tsuchiya K, Hosojima S, Hara H, Kushiyama H, Mahib M, Kinoshita T, et al. Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes. Cell Rep. 2021;34:108887 pubmed publisher
	Abrahams V, Tang Z, Mor G, Guller S. NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells. J Reprod Immunol. 2020;142:103214 pubmed publisher
	Lichtenegger S, Stiehler J, Saiger S, Zauner A, Kleinhappl B, Bernecker C, et al. Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model. PLoS Negl Trop Dis. 2020;14:e0008840 pubmed publisher
	Israelov H, Ravid O, Atrakchi D, Rand D, Elhaik S, Bresler Y, et al. Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair. J Neuroinflammation. 2020;17:267 pubmed publisher
	Pathak S, Vambutas A. Autoimmune inner ear disease patient-associated 28-kDa proinflammatory IL-1β fragment results from caspase-7-mediated cleavage in vitro. JCI Insight. 2020;5: pubmed publisher
	Eyre R, Alferez D, Santiago Gómez A, Spence K, McConnell J, Hart C, et al. Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling. Nat Commun. 2019;10:5016 pubmed publisher
	Vogel K, Pierau M, Arra A, Lampe K, Schlueter D, Arens C, et al. Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus. Sci Rep. 2018;8:16904 pubmed publisher
	Clancy D, Sullivan G, Moran H, Henry C, Reeves E, McElvaney N, et al. Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing. Cell Rep. 2018;22:2937-2950 pubmed publisher
	Chambers S, O Neill C, Guduric Fuchs J, McLoughlin K, Liew A, Egan A, et al. The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1β. Stem Cells. 2018;36:834-843 pubmed publisher
	Uchino Y, Woodward A, Mauris J, Peterson K, Verma P, Nilsson U, et al. Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes. Immunology. 2018;154:490-499 pubmed publisher
	Reggiani F, Labanca V, Mancuso P, Rabascio C, Talarico G, Orecchioni S, et al. Adipose Progenitor Cell Secretion of GM-CSF and MMP9 Promotes a Stromal and Immunological Microenvironment That Supports Breast Cancer Progression. Cancer Res. 2017;77:5169-5182 pubmed publisher
	Nishijima N, Hirai T, Misato K, Aoyama M, Kuroda E, Ishii K, et al. Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific. Front Immunol. 2017;8:379 pubmed publisher
	Naschberger E, Geißdörfer W, Bogdan C, Tripal P, Kremmer E, Sturzl M, et al. Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity. J Cell Mol Med. 2017;21:1954-1966 pubmed publisher
	Garstkiewicz M, Strittmatter G, Grossi S, Sand J, Fenini G, Werner S, et al. Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression. Eur J Immunol. 2017;47:806-817 pubmed publisher
	Hadadi E, Zhang B, Baidžajevas K, Yusof N, Puan K, Ong S, et al. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep. 2016;6:39035 pubmed publisher
	Huang J, Woods P, Normolle D, Goff J, Benos P, Stehle C, et al. Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals. Breast Cancer Res Treat. 2017;161:229-243 pubmed publisher
	Li W, Hua L, Qu P, Yan W, Ming C, Jun Y, et al. NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis. Arch Med Sci. 2016;12:950-958 pubmed
	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
	Oliver R, Lovric V, Yu Y, Christou C, Aiken S, Cooper J, et al. Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue. PLoS ONE. 2015;10:e0136514 pubmed publisher
	Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, et al. Neutrophil elastase promotes interleukin-1Î² secretion from human coronary endothelium. J Biol Chem. 2015;290:24067-78 pubmed publisher
	Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, et al. Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses. PLoS Pathog. 2015;11:e1005019 pubmed publisher
	Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, et al. IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation. PLoS Biol. 2012;10:e1001395 pubmed publisher
	Nicolaidou V, Wong M, Redpath A, Ersek A, Baban D, Williams L, et al. Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation. PLoS ONE. 2012;7:e39871 pubmed publisher
	Zielinski C, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, et al. Pathogen-induced human TH17 cells produce IFN-? or IL-10 and are regulated by IL-1?. Nature. 2012;484:514-8 pubmed publisher
	Aliahmadi E, Gramlich R, Grützkau A, Hitzler M, Krüger M, Baumgrass R, et al. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Eur J Immunol. 2009;39:1221-30 pubmed publisher
	Li J, Yin H, Yuan J. Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity. J Cell Biol. 2008;181:321-33 pubmed publisher
	Acosta Rodriguez E, Napolitani G, Lanzavecchia A, Sallusto F. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat Immunol. 2007;8:942-9 pubmed
	van Grevenstein W, Hofland L, van Rossen M, van Koetsveld P, Jeekel J, van Eijck C. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig Dis Sci. 2007;52:2775-83 pubmed
	Lee K, Ho S, Lin H, Lin S, Liu C, Huang C, et al. Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway. Am J Respir Cell Mol Biol. 2006;35:407-14 pubmed
	Banks W, Farr S, La Scola M, Morley J. Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum. J Pharmacol Exp Ther. 2001;299:536-41 pubmed
	Yates S, Burgess L, Kocsis Angle J, Antal J, Dority M, Embury P, et al. Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia. J Neurochem. 2000;74:1017-25 pubmed

image

image 1 :

Detection of Human IL-1ss/IL-1F2 antibody by Western Blot.

product information

brand :

R&D Systems

master code :

MAB201

SKU :

MAB201-100

product name :

IL-1 beta/IL-1F2 Antibody

description :

The Human IL-1 beta/IL-1F2 Antibody from R&D Systems is a mouse monoclonal antibody to IL-1 beta/IL-1F2. This antibody reacts with human. The Human IL-1 beta/IL-1F2 Antibody has been validated for the following applications: Western Blot, Simple Western, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready.

target :

IL-1 beta/IL-1F2

category :

Primary Antibodies

unit sizes :

100 ug (also 25 ug, 500 ug)

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

clonality :

Monoclonal

clone :

8516

concentration :

LYOPH

conjugate :

Unconjugated

host :

Mouse

immunogen :

E. coli -derived recombinant human IL-1 beta/IL-1F2, aa 117-269, Accession # P01584

isotype :

IgG1

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human

specificity :

Detects human IL-1 beta/IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta and rpIL-1 beta and no cross-reactivity with rrIL-1 beta , rmIL-1 alpha , rhIL-1ra, rmIL-1ra, or rrIL-1 alpha .

gene symbol :

IL1B

endotoxin note :

<0.10 EU per 1 µg of the antibody by the LAL method.

accessionNumbers :

P01584

applications :

Western Blot, Simple Western, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

USD :

453 USD

alt names :

catabolin, IL-1, IL1 beta, IL-1 beta, IL-1b, IL1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage