IL-1 alpha/IL-1F1 Antibody | R&D Systems MAB200-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

IL-1 alpha/IL-1F1 Antibody

catalog :

MAB200-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

499 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4414

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig s5f	Fernández Duran I, Quintanilla A, Tarrats N, Birch J, Hari P, Millar F, et al. Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence. Cell Death Differ. 2022;29:1267-1282 pubmed publisher
western blot; human; loading ...	Goncalves S, Yin K, Ito Y, Chan A, Olan I, Gough S, et al. COX2 regulates senescence secretome composition and senescence surveillance through PGE2. Cell Rep. 2021;34:108860 pubmed publisher
	Lee E, Kim S, Lee S, Jung J, Lee S, Na H, et al. Molecule-Resolved Visualization of Particulate Matter on Human Skin Using Multimodal Nonlinear Optical Imaging. Int J Mol Sci. 2021;22: pubmed publisher
	Gajawada P, Cetinkaya A, von Gerlach S, Kubin N, Burger H, Nabauer M, et al. Myocardial Accumulations of Reg3A, Reg3γ and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis. Int J Mol Sci. 2021;22: pubmed publisher
	Salti T, Khazim K, Haddad R, Campisi Pinto S, Bar Sela G, Cohen I. Glucose Induces IL-1α-Dependent Inflammation and Extracellular Matrix Proteins Expression and Deposition in Renal Tubular Epithelial Cells in Diabetic Kidney Disease. Front Immunol. 2020;11:1270 pubmed publisher
	Clausen B, Wirenfeldt M, Høgedal S, Frich L, Nielsen H, Schrøder H, et al. Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke. Acta Neuropathol Commun. 2020;8:81 pubmed publisher
	Glorieux C, Huang P. Regulation of CD137 expression through K-Ras signaling in pancreatic cancer cells. Cancer Commun (Lond). 2019;39:41 pubmed publisher
	Georgouli M, Herraiz C, Crosas Molist E, Fanshawe B, Maiques O, Perdrix A, et al. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment. Cell. 2019;176:757-774.e23 pubmed publisher
	Sun X, Shi B, Zheng H, Min L, Yang J, Li X, et al. Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway. Cell Death Dis. 2018;9:260 pubmed publisher
	Henry C, Martin S. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation. Mol Cell. 2017;65:715-729.e5 pubmed publisher
	Beltrami Moreira M, Vromman A, Sukhova G, Folco E, Libby P. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling. PLoS ONE. 2016;11:e0152474 pubmed publisher
	Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, et al. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem. 2015;290:3390-404 pubmed publisher
	McCarthy D, Clark R, Bartling T, Trebak M, Melendez J. Redox control of the senescence regulator interleukin-1? and the secretory phenotype. J Biol Chem. 2013;288:32149-59 pubmed publisher
	Uribe Herranz M, Lian L, Hooper K, Milora K, Jensen L. IL-1R1 signaling facilitates Munro's microabscess formation in psoriasiform imiquimod-induced skin inflammation. J Invest Dermatol. 2013;133:1541-9 pubmed publisher
	Bellini A, Marini M, Bianchetti L, Barczyk M, Schmidt M, Mattoli S. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients. Mucosal Immunol. 2012;5:140-9 pubmed publisher
	Berda Haddad Y, Robert S, Salers P, Zekraoui L, Farnarier C, Dinarello C, et al. Sterile inflammation of endothelial cell-derived apoptotic bodies is mediated by interleukin-1?. Proc Natl Acad Sci U S A. 2011;108:20684-9 pubmed publisher
	Zepp J, Nold Petry C, Dinarello C, Nold M. Protection from RNA and DNA viruses by IL-32. J Immunol. 2011;186:4110-8 pubmed publisher
	Olaru F, Jensen L. Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signaling loop. J Invest Dermatol. 2010;130:1866-76 pubmed publisher
	Bhaumik D, Scott G, Schokrpur S, Patil C, Orjalo A, Rodier F, et al. MicroRNAs miR-146a/b negatively modulate the senescence-associated inflammatory mediators IL-6 and IL-8. Aging (Albany NY). 2009;1:402-11 pubmed
	Jung D, Che Z, Kim J, Kim K, Kim K, Williams D, et al. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int J Cancer. 2010;127:332-44 pubmed publisher
	Wu W, Mehta H, Chakrabarty K, Booth J, Duggan E, Patel K, et al. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J Immunol. 2009;183:5799-806 pubmed publisher
	Chang S, Su P, Lee T. Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway. Cytokine. 2009;45:32-8 pubmed publisher
	Nold M, Nold Petry C, Pott G, Zepp J, Saavedra M, Kim S, et al. Endogenous IL-32 controls cytokine and HIV-1 production. J Immunol. 2008;181:557-65 pubmed
	Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
	Biancotto A, Iglehart S, Lisco A, Vanpouille C, Grivel J, Lurain N, et al. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res Hum Retroviruses. 2008;24:453-62 pubmed publisher
	Weindl G, Naglik J, Kaesler S, Biedermann T, Hube B, Korting H, et al. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J Clin Invest. 2007;117:3664-72 pubmed
	Rao D, Tracey K, Pober J. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. J Immunol. 2007;179:6536-46 pubmed
	Pfleger C, Schloot N, ter Veld F. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J Immunol Methods. 2008;329:214-8 pubmed
	Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
	Biancotto A, Grivel J, Iglehart S, Vanpouille C, Lisco A, Sieg S, et al. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood. 2007;109:4272-9 pubmed
	Lee K, Ho S, Lin H, Lin S, Liu C, Huang C, et al. Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway. Am J Respir Cell Mol Biol. 2006;35:407-14 pubmed

image

image 1 :

Cell Proliferation Induced by IL-1a/IL-1F1 and Neutralization by Human IL-1a/IL-1F1 Antibody.

product information

brand :

R&D Systems, a Bio-Techne brand

catalog number base :

MAB200

SKU :

MAB200-100

product name :

IL-1 alpha/IL-1F1 Antibody

description :

The Human IL-1 alpha /IL-1F1 Antibody from R&D Systems is a mouse monoclonal antibody to IL-1 alpha/IL-1F1. This antibody reacts with human. The Human IL-1 alpha /IL-1F1 Antibody has been validated for the following applications: ELISA Capture (Matched Antibody Pair),Electrochemiluminescent Assay,ELISA Development,ELISA Microarray Development,Luminex Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry.

target :

IL-1 alpha/IL-1F1

unit size :

100 ug (also 25 ug, 500 ug)

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

clonality :

Monoclonal

clone :

4414

concentration :

LYOPH

conjugate :

Unconjugated

host :

Mouse

immunogen :

E. coli -derived recombinant human IL-1 alpha /IL-1F1, Ser113-Ala271, Accession # P01583

isotype :

IgG2a

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human

specificity :

Detects human IL-1 alpha /IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1ß, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha , -1ß, -3, -4, -5, -6, -7, -9, or -13.

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

gene symbol :

IL1A

endotoxin note :

<0.10 EU per 1 µg of the antibody by the LAL method.

accessionNumbers :

P01583

applications :

ELISA Capture (Matched Antibody Pair),Electrochemiluminescent Assay,ELISA Development,ELISA Microarray Development,Luminex Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry

product keywords :

MAB200, hIL-1a MAb Cl 4414, Mouse Anti-Human IL-1 alpha/IL-1F1 Monoclonal Antibody, Anti-IL-1 alpha, Anti-IL-1F1, Mouse Anti Human IL-1 alpha/IL-1F1 Monoclonal Antibody, Anti-hIL-1 alpha, MAB2OO, cytokinegrowthfact, IL1 alpha, IL-1 , IL-1 , IL-1 alfa, IL 1 alpha, IL1alpha, Interleukin 1 alpha, IL-1a, IL-1 a, IL-1, Interleukin-1 alpha, cytokine, cytokines, IL1F1, IL1, IL1A, Hematopoietin-1, Pro-Interleukin-1-Alpha, Preinterleukin 1 Alpha

USD :

469

USD 2025 :

499 USD

alt names :

BAF, Hematopoietin-1, IL1, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha

more info or order :

R&D Systems product webpage