Human CXCR5 Antibody | R&D Systems MAB190-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human CXCR5 Antibody

catalog :

MAB190-100

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

51505

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 40

Reference
Kren xe1 cs L, Kren xe1 cs D, Borb xe9 nyi Z, T xf3 th E, Nagy A, Piukovics K, et al. Comparison of Follicular Helper T-Cell Markers with the Expression of the Follicular Homing Marker CXCR5 in Peripheral T-Cell Lymphomas-A Reappraisal of Follicular Helper T-Cell Lymphomas. Int J Mol Sci. 2023;25: pubmed publisher
Allard Chamard H, Kaneko N, Bertocchi A, Sun N, Boucau J, Kuo H, et al. Extrafollicular IgD-CD27-CXCR5-CD11c- DN3 B cells infiltrate inflamed tissues in autoimmune fibrosis and in severe COVID-19. Cell Rep. 2023;42:112630 pubmed publisher
Kaneko N, Chen H, Perugino C, Maehara T, Munemura R, Yokomizo S, et al. Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sjögren's syndrome. Sci Rep. 2022;12:15427 pubmed publisher
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Kaneko N, Kuo H, Boucau J, Farmer J, Allard Chamard H, Mahajan V, et al. The Loss of Bcl-6 Expressing T Follicular Helper Cells and the Absence of Germinal Centers in COVID-19. SSRN. 2020;:3652322 pubmed publisher
Cassotta A, Paparoditis P, Geiger R, Mettu R, Landry S, Donati A, et al. Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin. J Exp Med. 2020;217: pubmed publisher
Facciotti F, Larghi P, Bosotti R, Vasco C, Gagliani N, Cordiglieri C, et al. Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 2020;117:7305-7316 pubmed publisher
Maehara T, Kaneko N, Perugino C, Mattoo H, Kers J, Allard Chamard H, et al. Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis. J Clin Invest. 2020;130:2451-2464 pubmed publisher
Syu G, Wang S, Ma G, Liu S, Pearce D, Prakash A, et al. Development and application of a high-content virion display human GPCR array. Nat Commun. 2019;10:1997 pubmed publisher
Yoshitomi H, Kobayashi S, Miyagawa Hayashino A, Okahata A, Doi K, Nishitani K, et al. Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat Commun. 2018;9:3762 pubmed publisher
Maehara T, Mattoo H, Mahajan V, Murphy S, Yuen G, Ishiguro N, et al. The expansion in lymphoid organs of IL-4+ BATF+ T follicular helper cells is linked to IgG4 class switching in vivo. Life Sci Alliance. 2018;1: pubmed publisher
Armas González E, Domínguez Luis M, Díaz Martín A, Arce Franco M, Castro Hernandez J, Danelon G, et al. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis. Arthritis Res Ther. 2018;20:114 pubmed publisher
Aqrawi L, Ivanchenko M, Bjork A, Ramírez Sepúlveda J, Imgenberg Kreuz J, Kvarnstrom M, et al. Diminished CXCR5 expression in peripheral blood of patients with Sjögren's syndrome may relate to both genotype and salivary gland homing. Clin Exp Immunol. 2018;192:259-270 pubmed publisher
Rao D, Gurish M, Marshall J, Slowikowski K, Fonseka C, Liu Y, et al. Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis. Nature. 2017;542:110-114 pubmed publisher
Mitkin N, Hook C, Schwartz A, Biswas S, Kochetkov D, Muratova A, et al. p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells. Sci Rep. 2015;5:9330 pubmed publisher
Chu R, Reczek D, Brondyk W. Capture-stabilize approach for membrane protein SPR assays. Sci Rep. 2014;4:7360 pubmed publisher
Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, et al. The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE. 2014;9:e106008 pubmed publisher
Humpert M, Pinto D, Jarrossay D, Thelen M. CXCR7 influences the migration of B cells during maturation. Eur J Immunol. 2014;44:694-705 pubmed publisher
Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol. 2013;131:1146-56, 1156.e1-5 pubmed publisher
Stenton G, Mackenzie L, Tam P, Cross J, Harwig C, Raymond J, et al. Characterization of AQX-1125, a small-molecule SHIP1 activator: Part 1. Effects on inflammatory cell activation and chemotaxis in vitro and pharmacokinetic characterization in vivo. Br J Pharmacol. 2013;168:1506-18 pubmed publisher
Sarra M, Cupi M, Monteleone I, Franzè E, Ronchetti G, Di Sabatino A, et al. IL-15 positively regulates IL-21 production in celiac disease mucosa. Mucosal Immunol. 2013;6:244-55 pubmed publisher
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Islam S, Chang D, Colvin R, Byrne M, McCully M, Moser B, et al. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat Immunol. 2011;12:167-77 pubmed publisher
Weiss N, Deboux C, Chaverot N, Miller F, Baron van Evercooren A, Couraud P, et al. IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells. J Neuroimmunol. 2010;223:131-4 pubmed publisher
Singh S, Singh R, Sharma P, Singh U, Rai S, Chung L, et al. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett. 2009;283:29-35 pubmed publisher
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Cagigi A, Mowafi F, Phuong Dang L, Tenner Racz K, Atlas A, Grutzmeier S, et al. Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood. 2008;112:4401-10 pubmed publisher
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product information

brand :

R&D Systems

master code :

MAB190

SKU :

MAB190-100

product name :

Human CXCR5 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human CXCR5 Antibody from R&D Systems is a mouse monoclonal antibody to CXCR5. This antibody reacts with human,rabbit,rat. The Human CXCR5 Antibody has been validated for the following applications: Biacore Binding Assay,Flow Cytometry,Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-reported.

target :

CXCR5

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

51505

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 8-25 ug/mL, Neutralization 0.25-1.5 ug/mL, Immunocytochemistry 8-25 ug/mL, CyTOF-reported

host :

Mouse

immunogen :

NS0 mouse myeloma cell line transfected with human CXCR5, Met1-Phe372, Accession # P32302

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Rabbit,Rat

specificity :

Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

gene symbol :

CXCR5

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Detection of CXCR5 antibody in CD19+ Human PBMCs antibody by Flow Cytometry.

accessionNumbers :

P32302

applications :

CyTOF-reported,Biacore Binding Assay,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry

extended description :

Clone 51505 was used by HLDA to establish CD designation

USD :

499 USD

product details :

Clone 51505 was used by HLDA to establish CD designation

alt names :

BLR1, BLR1MDR-15, Burkitt lymphoma receptor 1, Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5), Burkitt lymphoma receptor 1, GTP-binding protein, CD185, CD185 antigen, chemokine (C-X-C motif) receptor 5, C-X-C chemokine receptor type 5, CXCR5, CXC-R5, MDR15CXCR-5, MGC117347, Monocyte-derived receptor 15

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage