Human Dectin-1/CLEC7A Antibody | R&D Systems MAB1859 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human Dectin-1/CLEC7A Antibody

catalog :

MAB1859

quantity :

100 ug (also 25 ug)

price :

469 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

259931

reactivity :

human, mouse

application :

western blot, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 34

Reference
Dos Santos J, Moreno M, Teufel L, Chilibroste S, Keating S, Groh L, et al. Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity. Cell Rep. 2024;43:113932 pubmed publisher
Ochoa A, Congel J, Corley J, Janssen W, Nick J, Malcolm K, et al. Dectin-1-Independent Macrophage Phagocytosis of Mycobacterium abscessus. Int J Mol Sci. 2023;24: pubmed publisher
de Figueiredo A, Dos Santos J, Kischkel B, Ardiansyah E, Oosting M, Guimar xe3 es Matos G, et al. Genome-Wide Association Study Reveals CLEC7A and PROM1 as Potential Regulators of Paracoccidioides brasiliensis-Induction of Cytokine Production in Peripheral Blood Mononuclear Cells. J Fungi (Basel). 2023;9: pubmed publisher
Haji S, Ito T, Guenther C, Nakano M, Shimizu T, Mori D, et al. Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2. elife. 2022;11: pubmed publisher
Gringhuis S, Kaptein T, Remmerswaal E, Drewniak A, Wevers B, Theelen B, et al. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs. Nat Immunol. 2022;23:1735-1748 pubmed publisher
Rochereau N, Michaud E, Waeckel L, Killian M, Gayet R, Goguyer Deschaumes R, et al. Essential role of TOSO/FAIM3 in intestinal IgM reverse transcytosis. Cell Rep. 2021;37:110006 pubmed publisher
He X, Howard B, Liu Y, Neumann A, Li L, Menon N, et al. LYSMD3: A mammalian pattern recognition receptor for chitin. Cell Rep. 2021;36:109392 pubmed publisher
Zhu G, Lyu L, Yang H, Lee J, Sun J, Zhang J, et al. CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis. Invest Ophthalmol Vis Sci. 2021;62:28 pubmed publisher
Silva J, Rodrigues N, Thompson Souza G, Muniz V, Neves J, Figueiredo R. Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination. J Leukoc Biol. 2020;107:69-83 pubmed publisher
de Quaglia e Silva J, Della Coletta A, Gardizani T, Romagnoli G, Kaneno R, Dias Melicio L. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis. J Immunol Res. 2019;2019:1529189 pubmed publisher
Kan B, Michalski C, Fu H, Au H, Lee K, Marchant E, et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun. 2018;9:4822 pubmed publisher
Lugo Villarino G, Troegeler A, Balboa L, Lastrucci C, Duval C, Mercier I, et al. The C-Type Lectin Receptor DC-SIGN Has an Anti-Inflammatory Role in Human M(IL-4) Macrophages in Response to Mycobacterium tuberculosis. Front Immunol. 2018;9:1123 pubmed publisher
Kaisar M, Ritter M, del Fresno C, Jónasdóttir H, van der Ham A, Pelgrom L, et al. Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses. PLoS Biol. 2018;16:e2005504 pubmed publisher
Swidergall M, Solis N, Lionakis M, Filler S. EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans. Nat Microbiol. 2018;3:53-61 pubmed publisher
Yeh C, Horng H, Chou H, Tai H, Shen H, Hsieh S, et al. Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells. Int J Mol Sci. 2017;18: pubmed publisher
Smith I, Baker A, Christensen J, Boekhout T, Frøkiær H, Arneborg N, et al. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro. PLoS ONE. 2016;11:e0167410 pubmed publisher
Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, et al. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects. J Immunol. 2016;196:1272-83 pubmed publisher
Elder M, Webster S, Williams D, Gaston J, Goodall J. TSLP production by dendritic cells is modulated by IL-1Î² and components of the endoplasmic reticulum stress response. Eur J Immunol. 2016;46:455-63 pubmed publisher
Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, et al. Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization. J Immunol. 2015;195:1723-31 pubmed publisher
Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias Melício L, Balderramas H, et al. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. PLoS ONE. 2015;10:e0120948 pubmed publisher
Cohen Kedar S, Baram L, Elad H, Brazowski E, Guzner Gur H, Dotan I. Human intestinal epithelial cells respond to Î²-glucans via Dectin-1 and Syk. Eur J Immunol. 2014;44:3729-40 pubmed publisher
Heyl K, Klassert T, Heinrich A, MÃ¼ller M, Klaile E, Dienemann H, et al. Dectin-1 is expressed in human lung and mediates the proinflammatory immune response to nontypeable Haemophilus influenzae. MBio. 2014;5:e01492-14 pubmed publisher
Klassert T, Hanisch A, Brauer J, Klaile E, Heyl K, Mansour M, et al. Modulatory role of vitamin A on the Candida albicans-induced immune response in human monocytes. Med Microbiol Immunol. 2014;203:415-24 pubmed publisher
Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, et al. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis. Cytokine. 2014;69:102-9 pubmed publisher
Baranov M, ter Beest M, Reinieren Beeren I, Cambi A, Figdor C, van den Bogaart G. Podosomes of dendritic cells facilitate antigen sampling. J Cell Sci. 2014;127:1052-1064 pubmed publisher
Lefevre L, Lugo Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, et al. The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum. Immunity. 2013;38:1038-49 pubmed publisher
Ifrim D, Joosten L, Kullberg B, Jacobs L, Jansen T, Williams D, et al. Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway. J Immunol. 2013;190:4129-35 pubmed publisher
Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, et al. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production. PLoS ONE. 2012;7:e50589 pubmed publisher
Kassianos A, Hardy M, Ju X, Vijayan D, Ding Y, Vulink A, et al. Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli. Eur J Immunol. 2012;42:1512-22 pubmed publisher
Carmona E, Kottom T, Hebrink D, Moua T, Singh R, Pagano R, et al. Glycosphingolipids mediate pneumocystis cell wall β-glucan activation of the IL-23/IL-17 axis in human dendritic cells. Am J Respir Cell Mol Biol. 2012;47:50-9 pubmed publisher
Gringhuis S, Kaptein T, Wevers B, Theelen B, van der Vlist M, Boekhout T, et al. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1? via a noncanonical caspase-8 inflammasome. Nat Immunol. 2012;13:246-54 pubmed publisher
Elsori D, Yakubenko V, Roome T, Thiagarajan P, Bhattacharjee A, Yadav S, et al. Protein kinase C? is a critical component of Dectin-1 signaling in primary human monocytes. J Leukoc Biol. 2011;90:599-611 pubmed publisher
Gringhuis S, Wevers B, Kaptein T, van Capel T, Theelen B, Boekhout T, et al. Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2. PLoS Pathog. 2011;7:e1001259 pubmed publisher
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product information

brand :

R&D Systems

master code :

MAB1859

SKU :

MAB1859

product name :

Human Dectin-1/CLEC7A Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human Dectin-1/CLEC7A Antibody from R&D Systems is a mouse monoclonal antibody to Dectin-1/CLEC7A. This antibody reacts with human,mouse. The Human Dectin-1/CLEC7A Antibody has been validated for the following applications: Immunocytochemistry,Functional Assay,Bioassay,Blocking,Flow Cytometry,Immunoprecipitation,Western Blot,Neutralization,Immunohistochemistry-Paraffin,Blockade of Receptor-ligand Interaction,CyTOF-ready.

target :

Dectin-1/CLEC7A

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

259931

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Flow Cytometry 2.5 ug/10^6 cells, Blockade of Receptor-ligand Interaction, Immunocytochemistry 8-25 ug/mL, CyTOF-ready

host :

Mouse

immunogen :

Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A, Thr66-Met201, Accession # NP_072092

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Mouse

specificity :

Detects human Dectin-1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

gene symbol :

CLEC7A

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Dectin-1/CLEC7A antibody in Human Dendritic Cells by Immunocytochemistry (ICC).

accessionNumbers :

NP_072092

applications :

Blockade of Receptor-ligand Interaction,CyTOF-ready,Bioassay,Blocking,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,Functional Assay

extended description :

Clone 259931 was used by HLDA to establish CD designation

USD :

469 USD

product details :

Clone 259931 was used by HLDA to establish CD designation

alt names :

Beta-glucan receptor, BGR, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, dectin-1, Dectin1, DECTIN1CANDF4, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage