Human NKp46/NCR1 Antibody | R&D Systems MAB1850-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human NKp46/NCR1 Antibody

catalog :

MAB1850-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

479 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

195314

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, mass cytometry

more info or order :

R&D Systems product webpage

citations: 48

Published Application/Species/Sample/Dilution	Reference
mass cytometry; human; loading ...; fig 2d	Jiang Z, Qin L, Tang Y, Liao R, Shi J, He B, et al. Human induced-T-to-natural killer cells have potent anti-tumour activities. Biomark Res. 2022;10:13 pubmed publisher
immunohistochemistry; human; 10 ug/ml; loading ...; fig s2a8	Zimmer T, Broekaart D, Luinenburg M, Mijnsbergen C, Anink J, Sim N, et al. Balloon cells promote immune system activation in focal cortical dysplasia type 2b. Neuropathol Appl Neurobiol. 2021;47:826-839 pubmed publisher
	Baraldo S, Bonato M, Cassia S, Casolari P, De Ferrari L, Tin xe9 M, et al. Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD. Respir Res. 2024;25:315 pubmed publisher
	Nakazawa T, Morimoto T, Maeoka R, Yamada K, Matsuda R, Nakamura M, et al. Characterization of HIF-1α Knockout Primary Human Natural Killer Cells Including Populations in Allogeneic Glioblastoma. Int J Mol Sci. 2024;25: pubmed publisher
	Kim H, Kim H, Nam M, Park J, Lee M, Chung S, et al. Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model. Cancer Cell Int. 2023;23:219 pubmed publisher
	Nakazawa T, Maeoka R, Morimoto T, Matsuda R, Nakamura M, Nishimura F, et al. An efficient feeder-free and chemically-defined expansion strategy for highly purified natural killer cells derived from human cord blood. Regen Ther. 2023;24:32-42 pubmed publisher
	Ahmed A, K xf6 hler S, Klotz R, Giese N, Hackert T, Springfeld C, et al. Sex Differences in the Systemic and Local Immune Response of Pancreatic Cancer Patients. Cancers (Basel). 2023;15: pubmed publisher
	Abeynaike S, Huynh T, Mehmood A, Kim T, Frank K, Gao K, et al. Human Hematopoietic Stem Cell Engrafted IL-15 Transgenic NSG Mice Support Robust NK Cell Responses and Sustained HIV-1 Infection. Viruses. 2023;15: pubmed publisher
	Kaneko N, Chen H, Perugino C, Maehara T, Munemura R, Yokomizo S, et al. Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sjögren's syndrome. Sci Rep. 2022;12:15427 pubmed publisher
	Sodji Q, Nambiar D, Viswanathan V, von Eyben R, Colburg D, Binkley M, et al. The Combination of Radiotherapy and Complement C3a Inhibition Potentiates Natural Killer cell Functions Against Pancreatic Cancer. Cancer Res Commun. 2022;2:725-738 pubmed publisher
	Nakazawa T, Morimoto T, Maeoka R, Matsuda R, Nakamura M, Nishimura F, et al. Establishment of an efficient ex vivo expansion strategy for human natural killer cells stimulated by defined cytokine cocktail and antibodies against natural killer cell activating receptors. Regen Ther. 2022;21:185-191 pubmed publisher
	Myers J, Schirm D, Bendzick L, Hopps R, Selleck C, Hinderlie P, et al. Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion. JCI Insight. 2022;7: pubmed publisher
	Walle T, Kraske J, Liao B, Lenoir B, Timke C, von Bohlen Und Halbach E, et al. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8. Sci Adv. 2022;8:eabh4050 pubmed publisher
	Lieschke E, Wang Z, Chang C, Weeden C, Kelly G, Strasser A. Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence. Cell Death Differ. 2022;29:1004-1012 pubmed publisher
	Johnson B, Creason A, Stommel J, Keck J, Parmar S, Betts C, et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med. 2022;3:100525 pubmed publisher
	Rutigliani M, Bozzo M, Barberis A, Greppi M, Anelli E, Castellaro L, et al. Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection. Front Immunol. 2022;13:849140 pubmed publisher
	Sorrentino C, D Antonio L, Fieni C, Ciummo S, Di Carlo E. Colorectal Cancer-Associated Immune Exhaustion Involves T and B Lymphocytes and Conventional NK Cells and Correlates With a Shorter Overall Survival. Front Immunol. 2021;12:778329 pubmed publisher
	Ahmed A, K xf6 hler S, Klotz R, Giese N, Lasitschka F, Hackert T, et al. Peripheral blood and tissue assessment highlights differential tumor-circulatory gradients of IL2 and MIF with prognostic significance in resectable pancreatic ductal adenocarcinoma. Oncoimmunology. 2021;10:1962135 pubmed publisher
	Aschman T, Schneider J, Greuel S, Meinhardt J, Streit S, Goebel H, et al. Association Between SARS-CoV-2 Infection and Immune-Mediated Myopathy in Patients Who Have Died. JAMA Neurol. 2021;78:948-960 pubmed publisher
	Le D, Huynh T, BURT B, van Buren G, Abeynaike S, Zalfa C, et al. Natural killer cells and cytotoxic T lymphocytes are required to clear solid tumor in a patient-derived xenograft. JCI Insight. 2021;6: pubmed publisher
	Fiore P, Vacca P, Tumino N, Besi F, Pelosi A, Munari E, et al. Wilms' Tumor Primary Cells Display Potent Immunoregulatory Properties on NK Cells and Macrophages. Cancers (Basel). 2021;13: pubmed publisher
	Jin W, Shi K, He W, Sun J, Van Kaer L, Shi F, et al. Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition. Nat Neurosci. 2021;24:61-73 pubmed publisher
	Melaiu O, Chierici M, Lucarini V, Jurman G, Conti L, De Vito R, et al. Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma. Nat Commun. 2020;11:5992 pubmed publisher
	Choi J, Lim S, Kang J, Hwang S, Hwang K, Kim S, et al. Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors. Molecules. 2020;25: pubmed publisher
	Yamashita K, Iwatsuki M, Yasuda Yoshihara N, Morinaga T, Nakao Y, Harada K, et al. Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer. Br J Cancer. 2021;124:595-603 pubmed publisher
	Choi J, Lee E, Kim S, Park S, Oh S, Kang J, et al. Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer. BMC Cancer. 2019;19:817 pubmed publisher
	Mayassi T, Ladell K, Gudjonson H, McLaren J, Shaw D, Tran M, et al. Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease. Cell. 2019;176:967-981.e19 pubmed publisher
	Trotta A, Santagata S, Zanotta S, D Alterio C, Napolitano M, Rea G, et al. Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity. J Exp Clin Cancer Res. 2018;37:297 pubmed publisher
	Maron S, Alpert L, Kwak H, Lomnicki S, Chase L, Xu D, et al. Targeted Therapies for Targeted Populations: Anti-EGFR Treatment for EGFR-Amplified Gastroesophageal Adenocarcinoma. Cancer Discov. 2018;8:696-713 pubmed publisher
	Feng Y, Liao S, Wei C, Jia D, Wood K, Liu Q, et al. Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models. J Neuroinflammation. 2017;14:248 pubmed publisher
	Ikeda T, Seki S, Fujiwara M, Matsuura M, Ozaki Honda Y, Fujita S, et al. Low-risk population among patients with tumor-node-metastasis stage III/IV oral squamous cell carcinoma. Oncol Lett. 2017;14:3711-3716 pubmed publisher
	Shi J, Zhao J, Zhang X, Cheng Y, Hu J, Li Y, et al. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-β-dependent emperipolesis in HBV cirrhotic patients. Sci Rep. 2017;7:44544 pubmed publisher
	Messaoudene M, Fregni G, Enot D, Jacquelot N, Neves E, Germaud N, et al. NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution. Oncoimmunology. 2016;5:e1154251 pubmed publisher
	Hervier B, Perez M, Allenbach Y, Devilliers H, Cohen F, Uzunhan Y, et al. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome. J Immunol. 2016;197:1621-30 pubmed publisher
	Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, et al. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection. Immunity. 2016;45:131-44 pubmed publisher
	Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, et al. Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways. J Autoimmun. 2016;66:40-50 pubmed publisher
	Messaoudene M, PÃ©rier A, Fregni G, Neves E, Zitvogel L, Cremer I, et al. Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients. PLoS ONE. 2015;10:e0133363 pubmed publisher
	Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, et al. A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells. MAbs. 2014;6:728-39 pubmed publisher
	Carrega P, Bonaccorsi I, Di Carlo E, Morandi B, Paul P, Rizzello V, et al. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol. 2014;192:3805-15 pubmed publisher
	Messaoudene M, Fregni G, Fourmentraux Neves E, Chanal J, Maubec E, Mazouz Dorval S, et al. Mature cytotoxic CD56(bright)/CD16(+) natural killer cells can infiltrate lymph nodes adjacent to metastatic melanoma. Cancer Res. 2014;74:81-92 pubmed publisher
	Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, et al. Natural killer cells are characterized by the concomitantly increased interferon-? and cytotoxicity in acute resolved hepatitis B patients. PLoS ONE. 2012;7:e49135 pubmed publisher
	Keim S, Zoernig I, Spille A, Lahrmann B, Brand K, Herpel E, et al. Sequential metastases of colorectal cancer: Immunophenotypes and spatial distributions of infiltrating immune cells in relation to time and treatments. Oncoimmunology. 2012;1:593-599 pubmed
	Jaron Mendelson M, Yossef R, Appel M, Zilka A, Hadad U, Afergan F, et al. Dimerization of NKp46 receptor is essential for NKp46-mediated lysis: characterization of the dimerization site by epitope mapping. J Immunol. 2012;188:6165-74 pubmed publisher
	Durrenberger P, Ettorre A, Kamel F, Webb L, Sim M, Nicholas R, et al. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1). J Neuroinflammation. 2012;9:1 pubmed publisher
	Yu J, Mitsui T, Wei M, Mao H, Butchar J, Shah M, et al. NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human. J Clin Invest. 2011;121:1456-70 pubmed publisher
	Dulphy N, Haas P, Busson M, Belhadj S, Peffault De Latour R, Robin M, et al. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation. J Immunol. 2008;181:2227-37 pubmed
	Shi J, Tricot G, Garg T, Malaviarachchi P, Szmania S, Kellum R, et al. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood. 2008;111:1309-17 pubmed
	Warren H, Bettadapura J. A novel binding assay to assess specificity of monoclonal antibodies. J Immunol Methods. 2005;305:33-8 pubmed

product information

brand :

R&D Systems

master code :

MAB1850

SKU :

MAB1850-100

product name :

Human NKp46/NCR1 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human NKp46/NCR1 Antibody from R&D Systems is a mouse monoclonal antibody to NKp46/NCR1. This antibody reacts with human,mouse. The Human NKp46/NCR1 Antibody has been validated for the following applications: Immunohistochemistry,Bioassay,Binding Assay,Epitope Mapping,Neutralization,Functional Assay,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Flow Cytometry,Western Blot,Agonist Activity,CyTOF-reported,Dual RNAscope ISH-IHC Compatible.

target :

NKp46/NCR1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

195314

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Agonist Activity, Immunocytochemistry 8-25 ug/mL, CyTOF-reported, Dual RNAscope ISH-IHC 3-25 ug/mL

host :

Mouse

immunogen :

Mouse T cell hybridoma transfected with human NKp46/NCR1

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Mouse

specificity :

Detects human NKp46/NCR1 in direct ELISAs and Western blots.

gene symbol :

NCR1

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

NKp46/NCR1 antibody in NK-92 Human Cell Line and Human PBMCs by Immunocytochemistry (ICC).

applications :

Flow Cytometry,Western Blot,Agonist Activity,CyTOF-reported,Dual RNAscope ISH-IHC Compatible,Bioassay,Binding Assay,Epitope Mapping,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,Functional Assay,ELISA Capture

extended description :

Clone 195314 was used by HLDA to establish CD designation

USD :

479 USD

product details :

Clone 195314 was used by HLDA to establish CD designation

alt names :

CD335, CD335 antigen, hNKp46, Ly94, LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46), Lymphocyte antigen 94 homolog, MAR-1, natural cytotoxicity triggering receptor 1, Natural killer cell p46-related protein, NCR1, NK cell-activating receptor, NKp46, NKP46FLJ99094, NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage