product summary
Loading...
company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse Oct-3/4 Antibody
catalog :
MAB1759
quantity :
100 ug (also 25 ug)
price :
479 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
240408
reactivity :
human, mouse, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 48
Reference
Rakotomamonjy J, Rylaarsdam L, Fares Taie L, McDermott S, Davies D, Yang G, et al. PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model. Cell Rep. 2023;42:112845 pubmed publisher
Wang X, Sun J, Fu C, Liu C. Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9. Stem Cell Res. 2023;69:103077 pubmed publisher
Ding T, Gao Z, Liu H, Li Y, Liu S, Jian L. Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system. Stem Cell Res. 2023;67:103029 pubmed publisher
Zhou M, Huang P, Bai R, Liu X. Generation of a homozygous TAZ knockout hESCs line by CRISPR/Cas9 system. Stem Cell Res. 2022;64:102923 pubmed publisher
Bejoy J, Farry J, Peek J, Cabatu M, Williams F, Welch R, et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease. Stem Cell Res Ther. 2022;13:355 pubmed publisher
Farahi S, Hosseini S, Ghanbarian H, Hashemi S, Salehi M, Hosseini S. The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level. Stem Cells Int. 2022;2022:9346767 pubmed publisher
Nishio M, Matsuura T, Hibi S, Ohta S, Oka C, Sasai N, et al. Heterozygous loss of Zbtb38 leads to early embryonic lethality via the suppression of Nanog and Sox2 expression. Cell Prolif. 2022;55:e13215 pubmed publisher
Kwaśniak K, Czarnik Kwaśniak J, Malysheva K, Pogoda K, Korchynskyi O, Rybojad P, et al. LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells. Cells. 2022;11: pubmed publisher
Hota S, Rao K, Blair A, Khalilimeybodi A, Hu K, Thomas R, et al. Brahma safeguards canalization of cardiac mesoderm differentiation. Nature. 2022;602:129-134 pubmed publisher
Zhang S, Guo T, Song Y, Jiang M, Jiang Y, Dong T, et al. Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system. Stem Cell Res. 2021;59:102631 pubmed publisher
Bejoy J, Qian E, WOODARD L. Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells. STAR Protoc. 2021;2:100898 pubmed publisher
Ma S, Xu Q, Bai R, Dong T, Peng Z, Liu X. Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing. Stem Cell Res. 2021;55:102470 pubmed publisher
Liu X, Zhang S, Chang Y, Wu F, Bai R. Establishment of a KCNQ1 homozygous knockout human embryonic stem cell line by episomal vector-based CRISPR/Cas9 system. Stem Cell Res. 2021;55:102467 pubmed publisher
Wang W, Bai R, Song X. Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system. Stem Cell Res. 2021;55:102471 pubmed publisher
Getachew A, Yang Z, Huang X, Wu F, Liu Y, Yan C, et al. Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing. Stem Cell Res. 2021;54:102401 pubmed publisher
Zhao T, Bai R, Wu F, Lu W, Zhang J. Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing. Stem Cell Res. 2021;51:102156 pubmed publisher
Andreasson L, Evenbratt H, Mobini R, Simonsson S. Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces. J Biotechnol. 2021;325:173-178 pubmed publisher
Wu F, Li X, Bai R, Li Y, Gao J, Lan F. Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system. Stem Cell Res. 2020;48:101930 pubmed publisher
Wei L, Zhang J, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi005-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene. Stem Cell Res. 2020;47:101909 pubmed publisher
Wei L, Zhang J, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi002-A) from a patient with spinocerebellar ataxia type 3. Stem Cell Res. 2020;47:101898 pubmed publisher
COLLINS D, Hapke J, Aravalli R, Steer C. In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells. Hepat Med. 2020;12:79-92 pubmed publisher
Kaufman G, Kiburi N, Skrtic D. The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer. Biosci Rep. 2020;40: pubmed publisher
Zhang J, Wei L, Wang R, Li X, Liu Z. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi001-A) from a healthy male individual. Stem Cell Res. 2020;45:101809 pubmed publisher
Zhang J, Wei L, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi004-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene. Stem Cell Res. 2020;44:101741 pubmed publisher
Guo X, Tang Y, Zhang P, Li S, Chen Y, Qian B, et al. Effect of ectopic high expression of transcription factor OCT4 on the "stemness" characteristics of human bone marrow-derived mesenchymal stromal cells. Stem Cell Res Ther. 2019;10:160 pubmed publisher
Katsura Y, Ohara T, Noma K, Ninomiya T, Kashima H, Kato T, et al. A Novel Combination Cancer Therapy with Iron Chelator Targeting Cancer Stem Cells via Suppressing Stemness. Cancers (Basel). 2019;11: pubmed publisher
Ito N, Katoh K, Kushige H, Saito Y, Umemoto T, Matsuzaki Y, et al. Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency. Sci Rep. 2018;8:1634 pubmed publisher
Xu G, Guo D, Wu F, Abbas N, Lai K, Yuan F, et al. Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing. Stem Cell Res. 2018;27:38-41 pubmed publisher
Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N, et al. Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Stem Cell Res. 2018;27:5-9 pubmed publisher
Ninomiya T, Ohara T, Noma K, Katsura Y, Katsube R, Kashima H, et al. Iron depletion is a novel therapeutic strategy to target cancer stem cells. Oncotarget. 2017;8:98405-98416 pubmed publisher
Xu Y, Wu Y, Guo D, Gao G, Lai K, Yang F, et al. Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9. Stem Cell Res. 2017;22:29-32 pubmed publisher
Kaufman G, Skrtic D. Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix. Tissue Cell. 2017;49:401-409 pubmed publisher
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, et al. HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget. 2017;8:28074-28092 pubmed publisher
Wang P, Wan W, Xiong S, Feng H, Wu N. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer. Cell Death Discov. 2017;3:16105 pubmed publisher
Kotoku T, Kosaka K, Nishio M, Ishida Y, Kawaichi M, Matsuda E. CIBZ Regulates Mesodermal and Cardiac Differentiation of by Suppressing T and Mesp1 Expression in Mouse Embryonic Stem Cells. Sci Rep. 2016;6:34188 pubmed publisher
Vincent Z, Urakami K, Maruyama K, Yamaguchi K, Kusuhara M. CD133-positive cancer stem cells from Colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile. Genes Cancer. 2014;5:250-60 pubmed
Hagiwara K, Obayashi T, Sakayori N, Yamanishi E, Hayashi R, Osumi N, et al. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells. PLoS ONE. 2014;9:e84072 pubmed publisher
Cortes Dericks L, Yazd E, Mowla S, Schmid R, Karoubi G. Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis. Anticancer Res. 2013;33:5365-73 pubmed
Pekkanen Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto Setala K. The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Stem Cells Int. 2012;2012:875059 pubmed publisher
Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, et al. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells. Mol Ther. 2011;19:584-93 pubmed publisher
Klim J, Li L, Wrighton P, Piekarczyk M, Kiessling L. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat Methods. 2010;7:989-94 pubmed publisher
Angel M, Yanik M. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS ONE. 2010;5:e11756 pubmed publisher
Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, et al. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol. 2010;202:193.e1-193.e13 pubmed publisher
Ware C, Wang L, Mecham B, Shen L, Nelson A, Bar M, et al. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell. 2009;4:359-69 pubmed publisher
Hu T, Liu S, Breiter D, Wang F, Tang Y, Sun S. Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Cancer Res. 2008;68:6533-40 pubmed publisher
Cordey M, Limacher M, Kobel S, Taylor V, Lutolf M. Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays. Stem Cells. 2008;26:2586-94 pubmed publisher
Hohenstein K, Pyle A, Chern J, Lock L, Donovan P. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Stem Cells. 2008;26:1436-43 pubmed publisher
Rajesh D, Chinnasamy N, Mitalipov S, Wolf D, Slukvin I, Thomson J, et al. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells. 2007;25:490-9 pubmed
product information
brand :
R&D Systems
master code :
MAB1759
SKU :
MAB1759
product name :
Human/Mouse Oct-3/4 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse Oct-3/4 Antibody from R&D Systems is a rat monoclonal antibody to Oct-3/4. This antibody reacts with human,mouse,primate - macaca mulatta (rhesus macaque). The Human/Mouse Oct-3/4 Antibody has been validated for the following applications: Immunocytochemistry,Bioassay,Flow Cytometry,Western Blot,Immunohistochemistry,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Intracellular Staining by Flow Cytometry,CyTOF-ready.
target :
Oct-3/4
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
240408
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
host :
Rat
immunogen :
E. coli -derived recombinant human Oct-3/4, Met1-Asn265 (Met262Leu), Accession # Q01860
isotype :
IgG2b
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,Primate - Macaca mulatta (Rhesus Macaque)
specificity :
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
gene symbol :
POU5F1
top caption :
Detection of Human Oct-3/4 antibody by Western Blot.
accessionNumbers :
Q01860
applications :
Intracellular Staining by Flow Cytometry,CyTOF-ready,Bioassay,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
USD :
479 USD
alt names :
3-Oct, OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1, Oct4, Otf3g, Otf4, Pou5f1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.