Human HMGB1/HMG-1 Antibody | R&D Systems MAB1690-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human HMGB1/HMG-1 Antibody

catalog :

MAB1690-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

499 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

115603

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 34

Reference
Presto P, Ji G, Ponomareva O, Ponomarev I, Neugebauer V. Hmgb1 Silencing in the Amygdala Inhibits Pain-Related Behaviors in a Rat Model of Neuropathic Pain. Int J Mol Sci. 2023;24: pubmed publisher
Kitaoka S, Tomohiro A, Ukeshima S, Liu K, Wake H, Kimura S, et al. Repeated Social Defeat Stress Induces HMGB1 Nuclear Export in Prefrontal Neurons, Leading to Social Avoidance in Mice. Cells. 2023;12: pubmed publisher
Li Y, Feng Y, Liu L, Ke J, Long X. Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis <em>via</em> NF-κB signaling pathway. Eur J Histochem. 2022;66: pubmed publisher
Simpson J, Spann K, Phipps S. MLKL Regulates Rapid Cell Death-independent HMGB1 Release in RSV Infected Airway Epithelial Cells. Front Cell Dev Biol. 2022;10:890389 pubmed publisher
Chen M, Zhou Y, Xue M, Zhu R, Jing L, Lin L, et al. Anti-HMGB1 antibody is a potential characteristic autoantibody for Sjögren's syndrome. Sci Rep. 2022;12:6020 pubmed publisher
You F, Zhang J, Cheng F, Zou K, Zhang X, Chen J. ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2-Beclin 1 Complex in Cervical Cancer Ca Ski Cells. Int J Mol Sci. 2021;22: pubmed publisher
Hisaoka Nakashima K, Azuma H, Ishikawa F, Nakamura Y, Wang D, Liu K, et al. Corticosterone Induces HMGB1 Release in Primary Cultured Rat Cortical Astrocytes: Involvement of Pannexin-1 and P2X7 Receptor-Dependent Mechanisms. Cells. 2020;9: pubmed publisher
Begum S, Moreau F, Leon Coria A, Chadee K. Entamoeba histolytica stimulates the alarmin molecule HMGB1 from macrophages to amplify innate host defenses. Mucosal Immunol. 2020;13:344-356 pubmed publisher
Cai X, Biswas I, Panicker S, Giri H, Rezaie A. Activated protein C inhibits lipopolysaccharide-mediated acetylation and secretion of high-mobility group box 1 in endothelial cells. J Thromb Haemost. 2019;17:803-817 pubmed publisher
Corneth O, Schaper F, Luk F, Asmawidjaja P, Mus A, Horst G, et al. Lack of IL-17 Receptor A signaling aggravates lymphoproliferation in C57BL/6 lpr mice. Sci Rep. 2019;9:4032 pubmed publisher
Tangerås L, Silva G, Stødle G, Gierman L, Skei B, Collett K, et al. Placental inflammation by HMGB1 activation of TLR4 at the syncytium. Placenta. 2018;72-73:53-61 pubmed publisher
Davies J, Apta B, Harper M. Cross-reactivity of anti-HMGB1 antibodies for HMGB2. J Immunol Methods. 2018;456:72-76 pubmed publisher
Simon D, ANEJA R, Alexander H, Bell M, Bayir H, Kochanek P, et al. Minocycline Attenuates High Mobility Group Box 1 Translocation, Microglial Activation, and Thalamic Neurodegeneration after Traumatic Brain Injury in Post-Natal Day 17 Rats. J Neurotrauma. 2018;35:130-138 pubmed publisher
Haruma J, Teshigawara K, Hishikawa T, Wang D, Liu K, Wake H, et al. Anti-high mobility group box-1 (HMGB1) antibody attenuates delayed cerebral vasospasm and brain injury after subarachnoid hemorrhage in rats. Sci Rep. 2016;6:37755 pubmed publisher
Regan J, Kannan P, Kemp M, Kramer B, Newnham J, Jobe A, et al. Damage-Associated Molecular Pattern and Fetal Membrane Vascular Injury and Collagen Disorganization in Lipopolysaccharide-Induced Intra-amniotic Inflammation in Fetal Sheep. Reprod Sci. 2016;23:69-80 pubmed publisher
Walko T, Di Caro V, Piganelli J, Billiar T, Clark R, ANEJA R. Poly(ADP-ribose) polymerase 1-sirtuin 1 functional interplay regulates LPS-mediated high mobility group box 1 secretion. Mol Med. 2015;20:612-24 pubmed publisher
Moser B, Megerle A, Bekos C, Janik S, Szerafin T, Birner P, et al. Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH. PLoS ONE. 2014;9:e106440 pubmed publisher
Leite P, Lima Araújo K, França G, Lagrota Candido J, Santos W, Quirico Santos T. Implant of polymer containing pentacyclic triterpenes from Eugenia punicifolia inhibits inflammation and activates skeletal muscle remodeling. Arch Immunol Ther Exp (Warsz). 2014;62:483-91 pubmed publisher
Moser B, Janik S, Schiefer A, Müllauer L, Bekos C, Scharrer A, et al. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. PLoS ONE. 2014;9:e94118 pubmed publisher
Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, et al. A dual-role of Gu-4 in suppressing HMGB1 secretion and blocking HMGB1 pro-inflammatory activity during inflammation. PLoS ONE. 2014;9:e89634 pubmed publisher
Suwara M, Green N, Borthwick L, Mann J, Mayer Barber K, Barron L, et al. IL-1α released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts. Mucosal Immunol. 2014;7:684-93 pubmed publisher
Liou T, Adler F, Keogh R, Li Y, Jensen J, Walsh W, et al. Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis. PLoS ONE. 2012;7:e42748 pubmed publisher
Sperling T, Ołdak M, Walch Rückheim B, Wickenhauser C, Doorbar J, Pfister H, et al. Human papillomavirus type 8 interferes with a novel C/EBP?-mediated mechanism of keratinocyte CCL20 chemokine expression and Langerhans cell migration. PLoS Pathog. 2012;8:e1002833 pubmed publisher
Colombo B, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi M, et al. Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS ONE. 2012;7:e31903 pubmed publisher
Donnelly O, Errington Mais F, Steele L, Hadac E, Jennings V, Scott K, et al. Measles virus causes immunogenic cell death in human melanoma. Gene Ther. 2013;20:7-15 pubmed publisher
Ali I, Gruenloh S, Gao Y, Clough A, Falck J, Medhora M, et al. Protection by 20-5,14-HEDGE against surgically induced ischemia reperfusion lung injury in rats. Ann Thorac Surg. 2012;93:282-8 pubmed publisher
Vitali R, Stronati L, Negroni A, Di Nardo G, Pierdomenico M, Del Giudice E, et al. Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease. Am J Gastroenterol. 2011;106:2029-40 pubmed publisher
Zhou J, Huang J, Poon V, Chen D, Chan C, Ng F, et al. Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection. J Hepatol. 2009;51:322-32 pubmed publisher
DAVE S, Tilstra J, Matsuoka K, Li F, DeMarco R, Beer Stolz D, et al. Ethyl pyruvate decreases HMGB1 release and ameliorates murine colitis. J Leukoc Biol. 2009;86:633-43 pubmed publisher
Rowe S, Jackson P, Liu G, Hardison M, Livraghi A, Solomon G, et al. Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Am J Respir Crit Care Med. 2008;178:822-31 pubmed publisher
Aychek T, Miller K, Sagi Assif O, Levy Nissenbaum O, Israeli Amit M, Pasmanik Chor M, et al. E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Int J Cancer. 2008;123:1741-50 pubmed publisher
Rao D, Tracey K, Pober J. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. J Immunol. 2007;179:6536-46 pubmed
Seki E, De Minicis S, Osterreicher C, Kluwe J, Osawa Y, Brenner D, et al. TLR4 enhances TGF-beta signaling and hepatic fibrosis. Nat Med. 2007;13:1324-32 pubmed
Zetterström C, Jiang W, Wähämaa H, Ostberg T, Aveberger A, Schierbeck H, et al. Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate. J Leukoc Biol. 2008;83:31-8 pubmed

product information

brand :

R&D Systems

master code :

MAB1690

SKU :

MAB1690-100

product name :

Human HMGB1/HMG-1 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human HMGB1/HMG-1 Antibody from R&D Systems is a mouse monoclonal antibody to HMGB1/HMG-1. This antibody reacts with human,mouse,rat. The Human HMGB1/HMG-1 Antibody has been validated for the following applications: Immunocytochemistry,ELISA Development,ELISA Standard,Immunodepletion,ELISA,Immunoprecipitation,Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Intracellular Staining by Flow Cytometry,Chromatin Immunoprecipitation (ChIP),CyTOF-ready.

target :

HMGB1/HMG-1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

115603

conjugate :

Unconjugated

dilution :

Western Blot 0.05 ug/mL, Immunohistochemistry 5-25 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready

host :

Mouse

immunogen :

E. coli -derived recombinant human HMGB1/HMG-1, Gly2-Glu215 (predicted), Accession # P09429

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Mouse,Rat

specificity :

Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.

gene symbol :

HMGB1

top caption :

Detection of Human HMGB1/HMG-1 antibody by Western Blot.

accessionNumbers :

P09429

applications :

Intracellular Staining by Flow Cytometry,Chromatin Immunoprecipitation (ChIP),CyTOF-ready,ELISA Development,ELISA Standard,Immunodepletion,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry

USD :

499 USD

alt names :

Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage