Human DC-SIGN/CD209 Antibody | R&D Systems MAB161-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human DC-SIGN/CD209 Antibody

catalog :

MAB161-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

469 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

120507

reactivity :

human, mouse, rat, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 48

Reference
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Guo L, Liang Y, Li H, Zheng H, Yang Z, Chen Y, et al. Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors. iScience. 2021;24:103426 pubmed publisher
Palmerin N, Aalam F, Nabiee R, Muniraju M, Ogembo J, Totonchy J. Suppression of DC-SIGN and gH Reveals Complex, Subset-Specific Mechanisms for KSHV Entry in Primary B Lymphocytes. Viruses. 2021;13: pubmed publisher
Zhao P, Xu L, Zhang Y, Cao H, Chen R, Wang B, et al. Expression of the human or porcine C-type lectins DC-SIGN/L-SIGN confers susceptibility to porcine epidemic diarrhea virus entry and infection in otherwise refractory cell lines. Microb Pathog. 2021;157:104956 pubmed publisher
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Hu B, Wang Z, Zeng H, Qi Y, Chen Y, Wang T, et al. Blockade of DC-SIGN+ Tumor-Associated Macrophages Reactivates Antitumor Immunity and Improves Immunotherapy in Muscle-Invasive Bladder Cancer. Cancer Res. 2020;80:1707-1719 pubmed publisher
Vogt M, Lahon A, Arya R, Spencer Clinton J, Rico Hesse R. Dengue viruses infect human megakaryocytes, with probable clinical consequences. PLoS Negl Trop Dis. 2019;13:e0007837 pubmed publisher
Xie M, Leroy H, Mascarau R, Woottum M, Dupont M, Ciccone C, et al. Cell-to-Cell Spreading of HIV-1 in Myeloid Target Cells Escapes SAMHD1 Restriction. MBio. 2019;10: pubmed publisher
Márquez S, Fernández J, Mancebo C, Herrero Sanchez C, Alonso S, Sandoval T, et al. Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns. Cell Rep. 2019;27:525-536.e4 pubmed publisher
Fedeli C, Torriani G, Galan Navarro C, Moraz M, Moreno H, Gerold G, et al. Axl Can Serve as Entry Factor for Lassa Virus Depending on the Functional Glycosylation of Dystroglycan. J Virol. 2018;92: pubmed publisher
Wang P, Li M, Lu W, Zhang D, Hu Q, Liu Y. DC-SIGN promotes Japanese encephalitis virus transmission from dendritic cells to T cells via virological synapses. Virol Sin. 2017;32:495-502 pubmed publisher
Dixon K, O Flynn J, Klar Mohamad N, Daha M, van Kooten C. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?. Eur J Immunol. 2017;47:470-480 pubmed publisher
Smith I, Baker A, Christensen J, Boekhout T, Frøkiær H, Arneborg N, et al. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro. PLoS ONE. 2016;11:e0167410 pubmed publisher
Xin G, Wei Z, Ji C, Zheng H, Gu J, Ma L, et al. Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release. Sci Rep. 2016;6:36222 pubmed publisher
SzatmÃ¡ri TÃ³th M, KristÃ³f E, VerÃ©b Z, Akhtar S, FacskÃ³ A, FÃ©sÃ¼s L, et al. Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration. Cell Death Dis. 2016;7:e2367 pubmed publisher
Zhang Y, El Far M, Dupuy F, Abdel Hakeem M, He Z, Procopio F, et al. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses. Sci Rep. 2016;6:29447 pubmed publisher
Suda Y, Fukushi S, Tani H, Murakami S, Saijo M, Horimoto T, et al. Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a vesicular stomatitis virus pseudotyping system. Arch Virol. 2016;161:1447-54 pubmed publisher
Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias Melício L, Balderramas H, et al. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. PLoS ONE. 2015;10:e0120948 pubmed publisher
El Awady A, Miles B, Scisci E, Kurago Z, Palani C, Arce R, et al. Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk. PLoS Pathog. 2015;10:e1004647 pubmed publisher
Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, et al. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. PLoS Pathog. 2014;10:e1004548 pubmed publisher
Rennick L, de Vries R, Carsillo T, Lemon K, van Amerongen G, Ludlow M, et al. Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates. J Virol. 2015;89:2192-200 pubmed publisher
Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose N, et al. Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues. PLoS ONE. 2014;9:e104390 pubmed publisher
Beck Z, Jagodzinski L, Eller M, Thelian D, Matyas G, Kunz A, et al. Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS ONE. 2013;8:e81002 pubmed publisher
Hesse C, Ginter W, Förg T, Mayer C, Baru A, Arnold Schrauf C, et al. In vivo targeting of human DC-SIGN drastically enhances CD8? T-cell-mediated protective immunity. Eur J Immunol. 2013;43:2543-53 pubmed publisher
Lang S, Bynoe M, Karki R, Tartell M, Means R. Kaposi's sarcoma-associated herpesvirus K3 and K5 proteins down regulate both DC-SIGN and DC-SIGNR. PLoS ONE. 2013;8:e58056 pubmed publisher
Mesman A, de Vries R, McQuaid S, Duprex W, De Swart R, Geijtenbeek T. A prominent role for DC-SIGN+ dendritic cells in initiation and dissemination of measles virus infection in non-human primates. PLoS ONE. 2012;7:e49573 pubmed publisher
Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, et al. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages. Blood. 2013;121:95-106 pubmed publisher
Emara M, Royer P, Mahdavi J, Shakib F, Ghaemmaghami A. Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. J Biol Chem. 2012;287:5756-63 pubmed publisher
Johnson T, McLellan J, Graham B. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J Virol. 2012;86:1339-47 pubmed publisher
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodríguez Rodrígues C, Remes Lenicov F, et al. Semen clusterin is a novel DC-SIGN ligand. J Immunol. 2011;187:5299-309 pubmed publisher
Hirbod T, Kaldensjö T, Broliden K. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS ONE. 2011;6:e25551 pubmed publisher
Bond E, Liang F, Sandgren K, Smed Sorensen A, Bergman P, Brighenti S, et al. Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations. J Invest Dermatol. 2012;132:114-23 pubmed publisher
Jain P, Manuel S, Khan Z, Ahuja J, Quann K, Wigdahl B. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells. J Virol. 2009;83:10908-21 pubmed publisher
Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S. Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation. Blood. 2009;113:5157-66 pubmed publisher
Kobayashi A, Weinberg V, Darragh T, Smith McCune K. Evolving immunosuppressive microenvironment during human cervical carcinogenesis. Mucosal Immunol. 2008;1:412-20 pubmed publisher
Iyori M, Ohtani M, Hasebe A, Totsuka Y, Shibata K. A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli. Biochem Biophys Res Commun. 2008;377:367-372 pubmed publisher
Nonaka M, Ma B, Murai R, Nakamura N, Baba M, Kawasaki N, et al. Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells. J Immunol. 2008;180:3347-56 pubmed
Wang J, Zhang Y, Wei J, Zhang X, Zhang B, Zhu Z, et al. Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response. Immunology. 2007;121:174-82 pubmed
Lai W, Sun P, Zhang J, Jennings A, Lalor P, Hubscher S, et al. Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles. Am J Pathol. 2006;169:200-8 pubmed
Okuma K, Boritz E, Walker J, Sarkar A, Alexander L, Rose J. Recombinant vesicular stomatitis viruses encoding simian immunodeficiency virus receptors target infected cells and control infection. Virology. 2006;346:86-97 pubmed
Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner E, et al. BCR/ABL promotes dendritic cell-mediated natural killer cell activation. Cancer Res. 2005;65:6409-17 pubmed
Beatty J, Islam S, North M, Knight S, Ogden C. Urine dendritic cells: a noninvasive probe for immune activity in bladder cancer?. BJU Int. 2004;94:1377-83 pubmed
Arrighi J, Pion M, Garcia E, Escola J, van Kooyk Y, Geijtenbeek T, et al. DC-SIGN-mediated infectious synapse formation enhances X4 HIV-1 transmission from dendritic cells to T cells. J Exp Med. 2004;200:1279-88 pubmed
Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, et al. NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs. Blood. 2004;104:3267-75 pubmed
Gardner L, Moffett A. Dendritic cells in the human decidua. Biol Reprod. 2003;69:1438-46 pubmed
Tailleux L, Schwartz O, Herrmann J, Pivert E, Jackson M, Amara A, et al. DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells. J Exp Med. 2003;197:121-7 pubmed
Wu L, Bashirova A, Martin T, Villamide L, Mehlhop E, Chertov A, et al. Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN. Proc Natl Acad Sci U S A. 2002;99:1568-73 pubmed

product information

brand :

R&D Systems

master code :

MAB161

SKU :

MAB161-100

product name :

Human DC-SIGN/CD209 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human DC-SIGN/CD209 Antibody from R&D Systems is a mouse monoclonal antibody to DC-SIGN/CD209. This antibody reacts with hamster,human,mouse,primate - cercopithecus aethiops (african green monkey),primate - macaca fascicularis (crab-eating monkey or cynomolgus macaque),primate - macaca mulatta (rhesus macaque),rat. The Human DC-SIGN/CD209 Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Blocking,ELISA Development,Neutralization,Functional Assay,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Adhesion Blockade,CyTOF-ready.

target :

DC-SIGN/CD209

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

120507

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Adhesion Blockade 5.0 ug/mL, CyTOF-ready

host :

Mouse

immunogen :

NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN/CD209

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Hamster,Human,Mouse,Primate - Cercopithecus aethiops (African Green Monkey),Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Primate - Macaca mulatta (Rhesus Macaque),Rat

specificity :

Detects human DC-SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

gene symbol :

CD209

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Detection of DC-SIGN antibody in Human DC-SIGN Transfected 3T3 Mouse Cell Line antibody by Flow Cytometry.

applications :

Adhesion Blockade,CyTOF-ready,Blocking,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,Functional Assay

extended description :

Clone 120507 was used by HLDA to establish CD designation

USD :

469 USD

product details :

Clone 120507 was used by HLDA to establish CD designation

alt names :

CD209, CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin, CD209 molecule, CDSIGNHIV gpl20-binding protein, CLEC4L, CLEC4LC-type lectin domain family 4 member L, DCSIGN, DC-SIGN1, DC-SIGN1C-type lectin domain family 4, member L, DC-SIGNMGC129965, Dendritic cell-specific ICAM-3-grabbing non-integrin 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage