product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human MICB Antibody
catalog :
MAB1599-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
469 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
236511
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, FACS
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
  • western blot; human; loading ...
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
Koh E, Lee H, Son W, Park G, Kim J, Bae J, et al. Combinatorial immunotherapy with gemcitabine and ex vivo-expanded NK cells induces anti-tumor effects in pancreatic cancer. Sci Rep. 2023;13:7656 pubmed publisher
Koh E, Lee H, Son W, Park G, Bae J, Park Y. Antitumor effects of NK cells expanded by activation pre‑processing of autologous feeder cells before irradiation in colorectal cancer. Oncol Lett. 2023;25:232 pubmed publisher
Joung J, Kirchgatterer P, Singh A, Cho J, Nety S, Larson R, et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun. 2022;13:1606 pubmed publisher
Chaouat A, Seliger B, Mandelboim O, Schmiedel D. The HHV-6A Proteins U20 and U21 Target NKG2D Ligands to Escape Immune Recognition. Front Immunol. 2021;12:714799 pubmed publisher
Petillo S, Capuano C, Molfetta R, Fionda C, Mekhloufi A, Pighi C, et al. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition. Cell Death Dis. 2021;12:836 pubmed publisher
Covino D, Desimio M, Doria M. Combinations of Histone Deacetylase Inhibitors with Distinct Latency Reversing Agents Variably Affect HIV Reactivation and Susceptibility to NK Cell-Mediated Killing of T Cells That Exit Viral Latency. Int J Mol Sci. 2021;22: pubmed publisher
Seidel E, Dassa L, Schuler C, Oiknine Djian E, Wolf D, Le Trilling V, et al. The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing. PLoS Pathog. 2021;17:e1008807 pubmed publisher
Seidel E, Dassa L, Kahlon S, Tirosh B, Halenius A, Seidel Malkinson T, et al. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain. Nat Commun. 2021;12:2061 pubmed publisher
Raynaud A, Desrumeaux K, Vidard L, Termine E, Baty D, Chames P, et al. Anti-NKG2D single domain-based antibodies for the modulation of anti-tumor immune response. Oncoimmunology. 2020;10:1854529 pubmed publisher
Møller S, Mellergaard M, Madsen M, Bermejo A, Jepsen S, Hansen M, et al. Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells. Front Immunol. 2020;11:1968 pubmed publisher
Diab M, Schmiedel D, Seidel E, Bacharach E, Mandelboim O. Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D. Viruses. 2020;12: pubmed publisher
Chang S, Kim H, Park C. Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens. Cells. 2020;9: pubmed publisher
Anft M, Netter P, Urlaub D, Prager I, Schaffner S, Watzl C. NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production. Cell Mol Immunol. 2020;17:347-355 pubmed publisher
McCarthy M, Lin D, Soga T, Adam J, O Callaghan C. Inosine pranobex enhances human NK cell cytotoxicity by inducing metabolic activation and NKG2D ligand expression. Eur J Immunol. 2020;50:130-137 pubmed publisher
Muñoz D, Yannone S, Daemen A, Sun Y, Vakar Lopez F, Kawahara M, et al. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging. JCI Insight. 2019;5: pubmed publisher
Giuliani E, Desimio M, Doria M. Hexamethylene bisacetamide impairs NK cell-mediated clearance of acute T lymphoblastic leukemia cells and HIV-1-infected T cells that exit viral latency. Sci Rep. 2019;9:4373 pubmed publisher
Dassa L, Seidel E, Oiknine Djian E, Yamin R, Wolf D, Le Trilling V, et al. The Human Cytomegalovirus Protein UL148A Downregulates the NK Cell-Activating Ligand MICA To Avoid NK Cell Attack. J Virol. 2018;92: pubmed publisher
Lee Y, Heo W, Nam J, Jeung Y, Bae J. The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells. J Radiat Res. 2018;59:245-252 pubmed publisher
McCarthy M, Moncayo G, Hiron T, Jakobsen N, Valli A, Soga T, et al. Purine nucleotide metabolism regulates expression of the human immune ligand MICA. J Biol Chem. 2018;293:3913-3924 pubmed publisher
Charpak Amikam Y, Kubsch T, Seidel E, Oiknine Djian E, Cavaletto N, Yamin R, et al. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20. Sci Rep. 2017;7:8661 pubmed publisher
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, et al. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells. Cell Death Dis. 2017;8:e2973 pubmed publisher
Weil S, Memmer S, Lechner A, Huppert V, Giannattasio A, Becker T, et al. Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma. Front Immunol. 2017;8:387 pubmed publisher
Raneros A, Puras A, Rodriguez R, Colado E, Bernal T, Anguita E, et al. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition. Oncotarget. 2017;8:31959-31976 pubmed publisher
Berhani O, Nachmani D, Yamin R, Schmiedel D, Bar On Y, Mandelboim O. Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5' Untranslated Region. J Immunol. 2017;198:3662-3670 pubmed publisher
Son C, Lee H, Koh E, Shin D, Bae J, Yang K, et al. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells. Sci Rep. 2016;6:32470 pubmed publisher
Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Önfelt B. Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J Immunol. 2015;195:3374-81 pubmed publisher
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, et al. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol. 2015;195:736-48 pubmed publisher
Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, et al. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer. 2015;15:351 pubmed publisher
Nielsen M, Dyring Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, et al. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol. 2015;135:1311-1319 pubmed publisher
Patursky Polischuk I, Kasir J, Miloslavski R, Hayouka Z, Hausner Hanochi M, Stolovich Rain M, et al. Reassessment of the role of TSC, mTORC1 and microRNAs in amino acids-meditated translational control of TOP mRNAs. PLoS ONE. 2014;9:e109410 pubmed publisher
Esteso G, Luzón E, Sarmiento E, Gómez Caro R, Steinle A, Murphy G, et al. Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA. J Immunol. 2014;193:1344-52 pubmed publisher
Nachmani D, Gutschner T, Reches A, Diederichs S, Mandelboim O. RNA-binding proteins regulate the expression of the immune activating ligand MICB. Nat Commun. 2014;5:4186 pubmed publisher
Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, et al. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathog. 2014;10:e1004058 pubmed publisher
Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, et al. MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathog. 2014;10:e1003963 pubmed publisher
Tsukerman P, Stern Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, et al. MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells. Cancer Res. 2012;72:5463-72 pubmed publisher
Nielsen N, Ødum N, Ursø B, Lanier L, Spee P. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE. 2012;7:e31959 pubmed publisher
Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto M, et al. DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction. Blood. 2011;117:4778-86 pubmed publisher
Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva Nilsson L. Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells. PLoS ONE. 2011;6:e16899 pubmed publisher
Björkström N, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, et al. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J Exp Med. 2011;208:13-21 pubmed publisher
Muntasell A, Magri G, Pende D, Angulo A, Lopez Botet M. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection. Blood. 2010;115:5170-9 pubmed publisher
Ohashi M, Eagle R, Trowsdale J. Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform. J Biol Chem. 2010;285:16408-15 pubmed publisher
Wu J, Atteridge C, Wang X, Seya T, Plymate S. Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation. Clin Cancer Res. 2009;15:632-40 pubmed publisher
Cerboni C, Ardolino M, Santoni A, Zingoni A. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood. 2009;113:2955-64 pubmed publisher
Boutet P, Agüera González S, Atkinson S, Pennington C, Edwards D, Murphy G, et al. Cutting edge: the metalloproteinase ADAM17/TNF-alpha-converting enzyme regulates proteolytic shedding of the MHC class I-related chain B protein. J Immunol. 2009;182:49-53 pubmed
Verhoeven D, de Hooge A, Mooiman E, Santos S, ten Dam M, Gelderblom H, et al. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol Immunol. 2008;45:3917-25 pubmed publisher
Tang K, He C, Zeng G, Wu J, Song G, Shi Y, et al. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem Biophys Res Commun. 2008;370:578-83 pubmed publisher
Skov S, Pedersen M, Andresen L, Straten P, Woetmann A, Odum N. Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B. Cancer Res. 2005;65:11136-45 pubmed
product information
brand :
R&D Systems
master code :
MAB1599
SKU :
MAB1599-100
product name :
Human MICB Antibody
unit size :
100 ug (also 25 ug, 500 ug)
seo description :
The Human MICB Antibody from R&D Systems is a mouse monoclonal antibody to MICB. This antibody reacts with human,mouse. The Human MICB Antibody has been validated for the following applications: ELISA Development,FACS,Flow Cytometry,Western Blot,Neutralization,Immunohistochemistry,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),CyTOF-ready,Knockout Validated.
target :
MICB
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
236511
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, CyTOF-ready, Knockout Validated 0.25 ug/10^6 cells, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
host :
Mouse
immunogen :
Mouse myeloma cell line NS0-derived recombinant human MICB, Ala23-Gly298, Accession # CAI18747
isotype :
IgG2b
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse
specificity :
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
gene symbol :
MICB
Antibody validation :
Knockout/Knockdown
top caption :
Detection of MICB antibody in K562 Human Cell Line antibody by Flow Cytometry.
accessionNumbers :
CAI18747
applications :
ELISA Capture (Matched Antibody Pair),CyTOF-ready,Knockout Validated,ELISA Development,FACS,Flow Cytometry,Immunohistochemistry,Western Blot,Neutralization,Immunocytochemistry
USD :
469 USD
alt names :
MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I polypeptide-related sequence B, MIC-B, PERB11.2MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.