Human/Mouse IL-12/IL-35 p35 Antibody | R&D Systems MAB1570-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human/Mouse IL-12/IL-35 p35 Antibody

catalog :

MAB1570-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

489 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

27537

reactivity :

human, mouse

application :

western blot, neutralization, immunoprecipitation, flow cytometry

more info or order :

R&D Systems product webpage

citations: 18

Reference
Martin N, Crupi M, Taha Z, Poutou J, Whelan J, Vallati S, et al. Engineering Rapalog-Inducible Genetic Switches Based on Split-T7 Polymerase to Regulate Oncolytic Virus-Driven Production of Tumour-Localized IL-12 for Anti-Cancer Immunotherapy. Pharmaceuticals (Basel). 2023;16: pubmed publisher
Luo Z, Sol xe4 ng C, Larsson R, Singh K. Interleukin-35 Prevents the Elevation of the M1/M2 Ratio of Macrophages in Experimental Type 1 Diabetes. Int J Mol Sci. 2022;23: pubmed publisher
Wetzel A, Scholtka B, Schumacher F, Rawel H, Geisendörfer B, Kleuser B. Epigenetic DNA Methylation of EBI3 Modulates Human Interleukin-35 Formation via NFkB Signaling: A Promising Therapeutic Option in Ulcerative Colitis. Int J Mol Sci. 2021;22: pubmed publisher
Mizoguchi I, Ohashi M, Hasegawa H, Chiba Y, Orii N, Inoue S, et al. EBV-induced gene 3 augments IL-23Rα protein expression through a chaperone calnexin. J Clin Invest. 2020;130:6124-6140 pubmed publisher
Abdulreda M, Berman D, Shishido A, Martin C, Hossameldin M, Tschiggfrie A, et al. Operational immune tolerance towards transplanted allogeneic pancreatic islets in mice and a non-human primate. Diabetologia. 2019;62:811-821 pubmed publisher
Zhang J, Mao T, Wang S, Wang D, Niu Z, Sun Z, et al. Interleukin-35 expression is associated with colon cancer progression. Oncotarget. 2017;8:71563-71573 pubmed publisher
Wang L, Zhu S, Xu G, Feng J, Han T, Zhao F, et al. Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection. J Biol Chem. 2016;291:16863-76 pubmed publisher
Halpert M, Konduri V, Liang D, Chen Y, Wing J, Paust S, et al. Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7. Stem Cells Dev. 2016;25:774-87 pubmed publisher
Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, et al. Dendritic cell SIRT1-HIF1Î± axis programs the differentiation of CD4+ T cells through IL-12 and TGF-Î²1. Proc Natl Acad Sci U S A. 2015;112:E957-65 pubmed publisher
Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, et al. Deltex1 antagonizes HIF-1Î± and sustains the stability of regulatory T cells in vivo. Nat Commun. 2015;6:6353 pubmed publisher
Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, et al. Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region. J Immunol. 2015;194:113-24 pubmed publisher
Wang Y, Dong J, Meng W, Ma J, Wang N, Wei J, et al. Effects of phased joint intervention on IL-35 and IL-17 expression levels in patients with portal hypertension. Int J Mol Med. 2014;33:1131-9 pubmed publisher
Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol. 2014;192:804-16 pubmed publisher
Olson B, Jankowska Gan E, Becker J, Vignali D, Burlingham W, McNeel D. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. J Immunol. 2012;189:5590-601 pubmed publisher
Seyerl M, Kirchberger S, Majdic O, Seipelt J, Jindra C, Schrauf C, et al. Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Eur J Immunol. 2010;40:321-9 pubmed publisher
Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes P, et al. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. J Allergy Clin Immunol. 2009;123:239-48 pubmed publisher
Liu T, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, et al. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur J Immunol. 2008;38:1012-23 pubmed publisher
Nagarajan S, Selvaraj P. Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination. Vaccine. 2006;24:2264-74 pubmed

product information

brand :

R&D Systems

master code :

MAB1570

SKU :

MAB1570-100

product name :

Human/Mouse IL-12/IL-35 p35 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human/Mouse IL-12/IL-35 p35 Antibody from R&D Systems is a mouse monoclonal antibody to IL-12/IL-35 p35. This antibody reacts with human,mouse. The Human/Mouse IL-12/IL-35 p35 Antibody has been validated for the following applications: Bioassay,ELISA Development,Flow Cytometry,Immunoprecipitation,Western Blot,Neutralization,Intracellular Staining by Flow Cytometry,CyTOF-ready.

target :

IL-12/IL-35 p35

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

27537

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready

host :

Mouse

immunogen :

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12/IL-35 p35, Arg23-Ser219, Accession # P29459

isotype :

IgG1

purity :

Protein A or G purified from ascites

species :

Human,Mouse

specificity :

Detects human IL-12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

gene symbol :

IL12A

accessionNumbers :

P29459

applications :

Intracellular Staining by Flow Cytometry,CyTOF-ready,Bioassay,ELISA Development,Flow Cytometry,Immunoprecipitation,Western Blot,Neutralization

USD :

489 USD

alt names :

CLMF, CLMF p35, CLMF1, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12, subunit p35, IL12A, IL-12A, IL35 subunit, IL35p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, NF cell stimulatory factor chain 1, NFSK, NK cell stimulatory factor chain 1, NKSF1, p35

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage