product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
catalog :
MAB1536
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
241809
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 67
Reference
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Zhao J, Rodger C, Allen G, Weidlich S, Ganley I. HIF1α-dependent mitophagy facilitates cardiomyoblast differentiation. Cell Stress. 2020;4:99-113 pubmed publisher
Toriuchi K, Kakita H, Tamura T, Takeshita S, Yamada Y, Aoyama M. Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions. J Neuroinflammation. 2020;17:141 pubmed publisher
Gavriilidis G, Ntoufa S, Papakonstantinou N, Kotta K, Koletsa T, Chartomatsidou E, et al. Stem cell factor is implicated in microenvironmental interactions and cellular dynamics of chronic lymphocytic leukemia. Haematologica. 2021;106:692-700 pubmed publisher
Sevilla Montero J, Bienes Martínez R, Labrousse Arias D, Fuertes Yebra E, Ordoñez A, Calzada M. pVHL-mediated regulation of the anti-angiogenic protein thrombospondin-1 decreases migration of Clear Cell Renal Carcinoma Cell Lines. Sci Rep. 2020;10:1175 pubmed publisher
Silagi E, Novais E, Bisetto S, Telonis A, Snuggs J, Le Maitre C, et al. Lactate Efflux From Intervertebral Disc Cells Is Required for Maintenance of Spine Health. J Bone Miner Res. 2019;: pubmed publisher
Rattila S, Dunk C, Im M, Grichenko O, Zhou Y, Yanez Mo M, et al. Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin Α5β1 Is a Modulator of Extravillous Trophoblast Functions. Cells. 2019;8: pubmed publisher
Gan N, Zhen X, Liu Y, Xu X, He C, Qiu J, et al. Regulation of phosphoribosyl ubiquitination by a calmodulin-dependent glutamylase. Nature. 2019;572:387-391 pubmed publisher
Pan H, Strickland A, Madhu V, Johnson Z, Chand S, Brody J, et al. RNA binding protein HuR regulates extracellular matrix gene expression and pH homeostasis independent of controlling HIF-1α signaling in nucleus pulposus cells. Matrix Biol. 2019;77:23-40 pubmed publisher
Jalili Firoozinezhad S, Prantil Baun R, Jiang A, Potla R, Mammoto T, Weaver J, et al. Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip. Cell Death Dis. 2018;9:223 pubmed publisher
Rao K, Sethi K, Ischia J, Gibson L, Galea L, Xiao L, et al. Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent. PLoS ONE. 2017;12:e0180028 pubmed publisher
Park C, Ivanova I, Kenneth N. XIAP upregulates expression of HIF target genes by targeting HIF1α for Lys63-linked polyubiquitination. Nucleic Acids Res. 2017;45:9336-9347 pubmed publisher
Fischer A, Miles S. Silencing HIF-1? induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells. Biochem Biophys Res Commun. 2017;490:176-181 pubmed publisher
Hernansanz Agustín P, Ramos E, Navarro E, Parada E, Sánchez López N, Peláez Aguado L, et al. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. Redox Biol. 2017;12:1040-1051 pubmed publisher
Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, et al. Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency. Cell Metab. 2017;25:1103-1117.e6 pubmed publisher
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, et al. HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget. 2017;8:28074-28092 pubmed publisher
van Gijsel Bonnello M, Baranger K, Benech P, Rivera S, Khrestchatisky M, De Reggi M, et al. Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine. PLoS ONE. 2017;12:e0175369 pubmed publisher
Basavarajappa H, Sulaiman R, Qi X, Shetty T, Sheik Pran Babu S, Sishtla K, et al. Ferrochelatase is a therapeutic target for ocular neovascularization. EMBO Mol Med. 2017;9:786-801 pubmed publisher
Labrousse Arias D, Martínez Alonso E, Corral Escariz M, Bienes Martínez R, Berridy J, Serrano Oviedo L, et al. VHL promotes immune response against renal cell carcinoma via NF-κB-dependent regulation of VCAM-1. J Cell Biol. 2017;216:835-847 pubmed publisher
Angara K, Rashid M, Shankar A, Ara R, Iskander A, Borin T, et al. Vascular mimicry in glioblastoma following anti-angiogenic and anti-20-HETE therapies. Histol Histopathol. 2017;32:917-928 pubmed publisher
Oh E, Kim J, Kim J, Kim S, Lee J, Hong S, et al. NQO1 inhibits proteasome-mediated degradation of HIF-1α. Nat Commun. 2016;7:13593 pubmed publisher
Wei Q, Bian Y, Yu F, Zhang Q, Zhang G, Li Y, et al. Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model. J Biomed Res. 2016;30:490-495 pubmed publisher
Bhogaraju S, Kalayil S, Liu Y, Bonn F, Colby T, Matic I, et al. Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination. Cell. 2016;167:1636-1649.e13 pubmed publisher
Gerriets V, Danzaki K, Kishton R, Eisner W, Nichols A, Saucillo D, et al. Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity. Eur J Immunol. 2016;46:1970-83 pubmed publisher
Suyama K, Silagi E, Choi H, Sakabe K, Mochida J, Shapiro I, et al. Circadian factors BMAL1 and ROR? control HIF-1? transcriptional activity in nucleus pulposus cells: implications in maintenance of intervertebral disc health. Oncotarget. 2016;7:23056-71 pubmed publisher
Orr A, Vargas L, Turk C, Baaten J, Matzen J, Dardov V, et al. Suppressors of superoxide production from mitochondrial complex III. Nat Chem Biol. 2015;11:834-6 pubmed publisher
Sun Y, George J, Rocha S. Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia. PLoS ONE. 2015;10:e0123649 pubmed publisher
Fischer C, Leithner K, Wohlkoenig C, Quehenberger F, Bertsch A, Olschewski A, et al. Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1α destabilization. Mol Cancer. 2015;14:4 pubmed publisher
Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, et al. Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-?B downregulation. Int J Cancer. 2014;134:2489-503 pubmed
Fujita N, Hirose Y, Tran C, Chiba K, Miyamoto T, Toyama Y, et al. HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells. FASEB J. 2014;28:2455-65 pubmed publisher
Bruzzese L, Fromonot J, By Y, Durand Gorde J, Condo J, Kipson N, et al. NF-?B enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors. Cell Signal. 2014;26:1060-7 pubmed publisher
Naranjo Suarez S, Carlson B, Tobe R, Yoo M, Tsuji P, Gladyshev V, et al. Regulation of HIF-1? activity by overexpression of thioredoxin is independent of thioredoxin reductase status. Mol Cells. 2013;36:151-7 pubmed publisher
Bett J, Ibrahim A, Garg A, Kelly V, Pedrioli P, Rocha S, et al. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem J. 2013;451:185-94 pubmed publisher
Kim H, Jeong J, Kim S, Park S, Chae H, Lee Y. Inhibition of endoplasmic reticulum stress alleviates lipopolysaccharide-induced lung inflammation through modulation of NF-?B/HIF-1? signaling pathway. Sci Rep. 2013;3:1142 pubmed publisher
Zhou J, Joplin D, Cross J, Templeton D. Sulforaphane inhibits prostaglandin E2 synthesis by suppressing microsomal prostaglandin E synthase 1. PLoS ONE. 2012;7:e49744 pubmed publisher
Zhdanov A, Dmitriev R, Papkovsky D. Bafilomycin A1 activates HIF-dependent signalling in human colon cancer cells via mitochondrial uncoupling. Biosci Rep. 2012;32:587-95 pubmed publisher
Naranjo Suarez S, Carlson B, Tsuji P, Yoo M, Gladyshev V, Hatfield D. HIF-independent regulation of thioredoxin reductase 1 contributes to the high levels of reactive oxygen species induced by hypoxia. PLoS ONE. 2012;7:e30470 pubmed publisher
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. Mol Biol Cell. 2011;22:4171-81 pubmed publisher
van Uden P, Kenneth N, Webster R, Müller H, Mudie S, Rocha S. Evolutionary conserved regulation of HIF-1β by NF-κB. PLoS Genet. 2011;7:e1001285 pubmed publisher
Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, et al. Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1? in MSCs. Biochem Biophys Res Commun. 2010;401:509-15 pubmed publisher
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Hickey M, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, et al. The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice. J Clin Invest. 2010;120:827-39 pubmed publisher
Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi G, et al. CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma. Cancer Res. 2008;68:7323-31 pubmed publisher
Zahm A, Bucaro M, Srinivas V, Shapiro I, Adams C. Oxygen tension regulates preosteocyte maturation and mineralization. Bone. 2008;43:25-31 pubmed publisher
Risbud M, Guttapalli A, Stokes D, Hawkins D, Danielson K, Schaer T, et al. Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem. 2006;98:152-9 pubmed
product information
brand :
R&D Systems
master code :
MAB1536
SKU :
MAB1536
product name :
Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody from R&D Systems is a mouse monoclonal antibody to HIF-1 alpha. This antibody reacts with bovine,human,mouse,ovine,rat. The Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Immunoprecipitation,Simple Western,Knockout Validated.
target :
HIF-1 alpha/HIF1A
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
241809
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Simple Western 10 ug/mL, Immunohistochemistry 5-25 ug/mL, Immunoprecipitation 1-3 ug/500 ug cell lysate, Knockout Validated
host :
Mouse
immunogen :
E. coli -derived recombinant human HIF-1 alpha/HIF1A, Arg575-Asn826, Accession # Q16665.1
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Bovine,Human,Mouse,Ovine,Rat
specificity :
Detects human, mouse, and rat HIF-1 alpha/HIF1A.
gene symbol :
HIF1A
Antibody validation :
Knockout/Knockdown
top caption :
Detection of Human, Mouse, and Rat HIF-1 alpha/HIF1A antibody by Western Blot.
accessionNumbers :
Q16665.1
applications :
Immunoprecipitation,Simple Western,Knockout Validated,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
USD :
489 USD
alt names :
AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF1, HIF1 alpha, HIF-1 alpha, HIF-1a, HIF1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.