product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human ULBP-3 Antibody
catalog :
MAB1517-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
469 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
166510
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 4d
Secchiari F, Nu xf1 ez S, Sierra J, Ziblat A, Regge M, Raffo Iraolagoitia X, et al. The MICA-NKG2D axis in clear cell renal cell carcinoma bolsters MICA as target in immuno-oncology. Oncoimmunology. 2022;11:2104991 pubmed publisher
Alekseeva N, Boyko A, Shevchenko M, Grechikhina M, Streltsova M, Alekseeva L, et al. Three-Dimensional Model Analysis Revealed Differential Cytotoxic Effects of the NK-92 Cell Line and Primary NK Cells on Breast and Ovarian Carcinoma Cell Lines Mediated by Variations in Receptor-Ligand Interactions and Soluble Factor Profiles. Biomedicines. 2024;12: pubmed publisher
Zhang Y, Luo F, Dong K. Soluble NKG2D ligands impair CD8+ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma. Oncol Lett. 2023;26:297 pubmed publisher
Koh E, Lee H, Son W, Park G, Kim J, Bae J, et al. Combinatorial immunotherapy with gemcitabine and ex vivo-expanded NK cells induces anti-tumor effects in pancreatic cancer. Sci Rep. 2023;13:7656 pubmed publisher
Koh E, Lee H, Son W, Park G, Bae J, Park Y. Antitumor effects of NK cells expanded by activation pre‑processing of autologous feeder cells before irradiation in colorectal cancer. Oncol Lett. 2023;25:232 pubmed publisher
Lee D, Lee M, Choi S, Park J, Ko E, Lee J, et al. ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer. Oncoimmunology. 2023;12:2190671 pubmed publisher
de Vries N, van de Haar J, Veninga V, Chalabi M, Ijsselsteijn M, van der Ploeg M, et al. γδ T cells are effectors of immunotherapy in cancers with HLA class I defects. Nature. 2023;613:743-750 pubmed publisher
Petillo S, Capuano C, Molfetta R, Fionda C, Mekhloufi A, Pighi C, et al. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition. Cell Death Dis. 2021;12:836 pubmed publisher
Cantoni C, Serra M, Parisi E, Azzarone B, Sementa A, Nasto L, et al. Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells. Oncoimmunology. 2021;10:1879530 pubmed publisher
Menasche B, Davis E, Wang S, Ouyang Y, Li S, Yu H, et al. PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes. Sci Adv. 2020;6: pubmed publisher
Garofalo S, Cocozza G, Porzia A, Inghilleri M, Raspa M, Scavizzi F, et al. Natural killer cells modulate motor neuron-immune cell cross talk in models of Amyotrophic Lateral Sclerosis. Nat Commun. 2020;11:1773 pubmed publisher
Sasawatari S, Okamoto Y, Kumanogoh A, Toyofuku T. Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells. J Immunol. 2020;204:1373-1385 pubmed publisher
McCarthy M, Lin D, Soga T, Adam J, O Callaghan C. Inosine pranobex enhances human NK cell cytotoxicity by inducing metabolic activation and NKG2D ligand expression. Eur J Immunol. 2020;50:130-137 pubmed publisher
Tremblay McLean A, Coenraads S, Kiani Z, Dupuy F, Bernard N. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function. BMC Immunol. 2019;20:8 pubmed publisher
Lee Y, Heo W, Nam J, Jeung Y, Bae J. The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells. J Radiat Res. 2018;59:245-252 pubmed publisher
López Cobo S, Pieper N, Campos Silva C, García Cuesta E, Reyburn H, Paschen A, et al. Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors. Oncoimmunology. 2018;7:e1392426 pubmed publisher
McCarthy M, Moncayo G, Hiron T, Jakobsen N, Valli A, Soga T, et al. Purine nucleotide metabolism regulates expression of the human immune ligand MICA. J Biol Chem. 2018;293:3913-3924 pubmed publisher
Sharma N, Trinidad C, Trembath A, Markiewicz M. NKG2D Signaling between Human NK Cells Enhances TACE-Mediated TNF-α Release. J Immunol. 2017;199:2865-2872 pubmed publisher
Charpak Amikam Y, Kubsch T, Seidel E, Oiknine Djian E, Cavaletto N, Yamin R, et al. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20. Sci Rep. 2017;7:8661 pubmed publisher
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, et al. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells. Cell Death Dis. 2017;8:e2973 pubmed publisher
Zumwalde N, Sharma A, Xu X, Ma S, Schneider C, Romero Masters J, et al. Adoptively transferred Vγ9Vδ2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model. JCI Insight. 2017;2: pubmed publisher
Cai X, Caballero Benítez A, Gewe M, Jenkins I, Drescher C, Strong R, et al. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells. Neoplasia. 2017;19:471-482 pubmed publisher
Weil S, Memmer S, Lechner A, Huppert V, Giannattasio A, Becker T, et al. Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma. Front Immunol. 2017;8:387 pubmed publisher
Raneros A, Puras A, Rodriguez R, Colado E, Bernal T, Anguita E, et al. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition. Oncotarget. 2017;8:31959-31976 pubmed publisher
Guo M, Wu T, Wan L. Cytotoxic activity of allogeneic natural killer cells on U251 glioma cells in vitro. Mol Med Rep. 2016;14:583-9 pubmed publisher
Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Önfelt B. Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J Immunol. 2015;195:3374-81 pubmed publisher
Song H, Kim Y, Park G, Kim Y, Kim S, Lee H, et al. Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. Int J Mol Med. 2015;36:1180-8 pubmed publisher
Cox S, Laza Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, et al. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity. Eur J Immunol. 2015;45:2324-34 pubmed publisher
Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, et al. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer. 2015;15:351 pubmed publisher
Kim G, Ha G, Bae J, Oh S, Kim S, Kang C. Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells. Oncol Lett. 2015;9:1641-1646 pubmed
Baragaño Raneros A, Martín Palanco V, Fernandez A, Rodriguez R, Fraga M, Lopez Larrea C, et al. Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia. Genes Immun. 2015;16:71-82 pubmed publisher
Morisaki T, Hirano T, Koya N, Kiyota A, Tanaka H, Umebayashi M, et al. NKG2D-directed cytokine-activated killer lymphocyte therapy combined with gemcitabine for patients with chemoresistant metastatic solid tumors. Anticancer Res. 2014;34:4529-38 pubmed
Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, et al. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol. 2014;193:950-60 pubmed publisher
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, et al. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol. 2014;192:5332-42 pubmed publisher
Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, et al. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathog. 2014;10:e1004058 pubmed publisher
Giuliani M, Bennaceur Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, et al. TLR ligands stimulation protects MSC from NK killing. Stem Cells. 2014;32:290-300 pubmed publisher
Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari M. NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation. Eur J Immunol. 2013;43:550-61 pubmed publisher
Tsukerman P, Stern Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, et al. MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells. Cancer Res. 2012;72:5463-72 pubmed publisher
MADRID A, Ganem D. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol. 2012;86:8693-704 pubmed publisher
Glässner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, et al. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab Invest. 2012;92:967-77 pubmed publisher
Nielsen N, Ødum N, Ursø B, Lanier L, Spee P. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE. 2012;7:e31959 pubmed publisher
Dupuy S, Lambert M, Zucman D, Choukem S, Tognarelli S, Pagès C, et al. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog. 2012;8:e1002486 pubmed publisher
Wang R, Jaw J, Stutzman N, Zou Z, Sun P. Natural killer cell-produced IFN-? and TNF-? induce target cell cytolysis through up-regulation of ICAM-1. J Leukoc Biol. 2012;91:299-309 pubmed publisher
DelaRosa O, Sanchez Correa B, Morgado S, Ramirez C, del Rio B, Menta R, et al. Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis. Stem Cells Dev. 2012;21:1333-43 pubmed publisher
Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva Nilsson L. Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells. PLoS ONE. 2011;6:e16899 pubmed publisher
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, et al. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Oncogene. 2011;30:2622-32 pubmed publisher
Fernández Messina L, Ashiru O, Agüera González S, Reyburn H, Vales Gomez M. The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells. J Cell Sci. 2011;124:321-7 pubmed publisher
Björkström N, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, et al. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J Exp Med. 2011;208:13-21 pubmed publisher
Nuckel H, Switala M, Sellmann L, Horn P, Durig J, Duhrsen U, et al. The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia. Leukemia. 2010;24:1152-9 pubmed publisher
Muntasell A, Magri G, Pende D, Angulo A, Lopez Botet M. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection. Blood. 2010;115:5170-9 pubmed publisher
Balsamo M, Scordamaglia F, Pietra G, Manzini C, Cantoni C, Boitano M, et al. Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity. Proc Natl Acad Sci U S A. 2009;106:20847-52 pubmed publisher
Ho V, Vanneman M, Kim H, Sasada T, Kang Y, Pasek M, et al. Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation. Proc Natl Acad Sci U S A. 2009;106:15825-30 pubmed publisher
Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, et al. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC. Int J Cancer. 2009;125:212-21 pubmed publisher
Cerboni C, Ardolino M, Santoni A, Zingoni A. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood. 2009;113:2955-64 pubmed publisher
Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, et al. Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int J Cancer. 2009;124:1594-604 pubmed publisher
Verhoeven D, de Hooge A, Mooiman E, Santos S, ten Dam M, Gelderblom H, et al. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol Immunol. 2008;45:3917-25 pubmed publisher
Shi J, Tricot G, Garg T, Malaviarachchi P, Szmania S, Kellum R, et al. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood. 2008;111:1309-17 pubmed
Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, et al. HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts. Blood. 2007;110:1207-14 pubmed
product information
master code :
MAB1517
SKU :
MAB1517-100
product name :
Human ULBP-3 Antibody
unit size :
100 ug (also 25 ug, 500 ug)
description :
The Human ULBP-3 Antibody from R&D Systems is a mouse monoclonal antibody to ULBP-3. This antibody reacts with human,mouse,transgenic mouse. The Human ULBP-3 Antibody has been validated for the following applications: Immunocytochemistry,Flow Cytometry,Immunohistochemistry,ELISA Development,Immunoprecipitation,Neutralization,Functional Assay,Blockade of Receptor-ligand Interaction,CyTOF-ready.
target :
ULBP-3
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
166510
concentration :
LYOPH
conjugate :
Unconjugated
host :
Mouse
immunogen :
BaF3 mouse pro-B cell line transfected with human ULBP-3
isotype :
IgG2a
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse,Transgenic Mouse
specificity :
Detects human ULBP-3 in ELISAs. Does not cross-react with recombinant human (rh) ULBP-1 or rhULBP-2.
gene symbol :
ULBP3
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Human ULBP-3 by Flow Cytometry
applications :
Immunocytochemistry,Flow Cytometry,Immunohistochemistry,ELISA Development,Immunoprecipitation,Neutralization,Functional Assay,Blockade of Receptor-ligand Interaction,CyTOF-ready
USD :
469 USD
alt names :
NKG2D ligand 3, RAET1N, UL16 binding protein 3, ULBP3
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.