Human ADAM10 Ectodomain Antibody | R&D Systems MAB1427-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human ADAM10 Ectodomain Antibody

catalog :

MAB1427-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

479 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

163003

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

R&D Systems product webpage

citations: 19

Reference
Saha N, Baek D, Mendoza R, Robev D, Xu Y, Goldgur Y, et al. Fully human monoclonal antibody targeting activated ADAM10 on colorectal cancer cells. Biomed Pharmacother. 2023;161:114494 pubmed publisher
Zatovicova M, Kajanová I, Takacova M, Jelenska L, Sedlakova O, Labudova M, et al. ADAM10 mediates shedding of carbonic anhydrase IX ectodomain non‑redundantly to ADAM17. Oncol Rep. 2023;49: pubmed publisher
Lischnig A, Bergqvist M, Ochiya T, L xe4 sser C. Quantitative Proteomics Identifies Proteins Enriched in Large and Small Extracellular Vesicles. Mol Cell Proteomics. 2022;21:100273 pubmed publisher
Colombo A, Sadler R, Llovera G, Singh V, Roth S, Heindl S, et al. Microbiota-derived short chain fatty acids modulate microglia and promote Aβ plaque deposition. elife. 2021;10: pubmed publisher
Martin Jaular L, Nevo N, Schessner J, Tkach M, Jouve M, Dingli F, et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J. 2021;40:e105492 pubmed publisher
Crescitelli R, L xe4 sser C, L xf6 tvall J. Isolation and characterization of extracellular vesicle subpopulations from tissues. Nat Protoc. 2021;16:1548-1580 pubmed publisher
Cocozza F, Nevo N, Piovesana E, Lahaye X, Buchrieser J, Schwartz O, et al. Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus. J Extracell Vesicles. 2020;10:e12050 pubmed publisher
Möller N, Ziesemer S, Hildebrandt P, Assenheimer N, Volker U, Hildebrandt J. S. aureus alpha-toxin monomer binding and heptamer formation in host cell membranes - Do they determine sensitivity of airway epithelial cells toward the toxin?. PLoS ONE. 2020;15:e0233854 pubmed publisher
Colombo A, Hsia H, Wang M, Kuhn P, Brill M, Canevazzi P, et al. Non-cell-autonomous function of DR6 in Schwann cell proliferation. EMBO J. 2018;37: pubmed publisher
Buchanan P, Boylan K, Walcheck B, Heinze R, Geller M, Argenta P, et al. Ectodomain shedding of the cell adhesion molecule Nectin-4 in ovarian cancer is mediated by ADAM10 and ADAM17. J Biol Chem. 2017;292:6339-6351 pubmed publisher
Atapattu L, Saha N, Chheang C, Eissman M, Xu K, Vail M, et al. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth. J Exp Med. 2016;213:1741-57 pubmed publisher
Zocchi M, Camodeca C, Nuti E, Rossello A, Ven xe8 R, Tosetti F, et al. ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing. Oncoimmunology. 2016;5:e1123367 pubmed publisher
Cai Z, Zhang A, Choksi S, Li W, Li T, Zhang X, et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res. 2016;26:886-900 pubmed publisher
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, et al. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol. 2015;195:736-48 pubmed publisher
Breshears L, Schlievert P, Peterson M. A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1). J Biol Chem. 2012;287:32578-87 pubmed
Zocchi M, Catellani S, Canevali P, Tavella S, Garuti A, Villaggio B, et al. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas. Blood. 2012;119:1479-89 pubmed publisher
Koenen R, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, et al. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases. Blood. 2009;113:4799-809 pubmed publisher
Janes P, Saha N, Barton W, Kolev M, Wimmer Kleikamp S, Nievergall E, et al. Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans. Cell. 2005;123:291-304 pubmed
Johansson M, Lye M, Barthel S, Duffy A, Annis D, Mosher D. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes. Am J Respir Cell Mol Biol. 2004;31:413-22 pubmed

product information

brand :

R&D Systems

master code :

MAB1427

SKU :

MAB1427-100

product name :

Human ADAM10 Ectodomain Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human ADAM10 Ectodomain Antibody from R&D Systems is a mouse monoclonal antibody to ADAM10. This antibody reacts with human,mouse. The Human ADAM10 Ectodomain Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Immunocytochemistry,Immunoprecipitation,Intracellular Staining by Flow Cytometry,CyTOF-ready.

target :

ADAM10

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

163003

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Immunoprecipitation 25 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready

host :

Mouse

immunogen :

Mouse myeloma cell line NS0-derived recombinant human ADAM10, Thr214-Glu672, Accession # O14672

isotype :

IgG2b

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Mouse

specificity :

Detects human ADAM10 in direct ELISAs and Western blots. In Western blots, shows less than 5% cross-reactivity with recombinant human (rh) ADAM8, 9, 15, 17/TACE, and recombinant mouse ADAM10.

gene symbol :

ADAM10

accessionNumbers :

O14672

applications :

Immunoprecipitation,Intracellular Staining by Flow Cytometry,CyTOF-ready,Flow Cytometry,Western Blot,Immunocytochemistry

USD :

479 USD

alt names :

a disintegrin and metalloprotease domain 10, a disintegrin and metalloproteinase domain 10, AD10, ADAM 10, ADAM metallopeptidase domain 10, CD156c, CD156c antigen, CDw156, disintegrin and metalloproteinase domain-containing protein 10, EC 3.4.24, HsT18717, kuz, Kuzbanian, Kuzbanian protein homolog, MADM, MADMEC 3.4.24.81, Mammalian disintegrin-metalloprotease

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage