Human B7-2/CD86 Antibody | R&D Systems MAB141-100 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human B7-2/CD86 Antibody

catalog :

MAB141-100

quantity :

100 ug (also 25 ug, 500 ug)

price :

469 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

37301

reactivity :

human

application :

western blot, immunocytochemistry, neutralization, flow cytometry

more info or order :

R&D Systems product webpage

citations: 15

Reference
Shankar S, Stolp J, Juvet S, Beckett J, Macklin P, Issa F, et al. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis. Nat Commun. 2022;13:3121 pubmed publisher
Jeong M, Zhou K, Park S, An H, Kwon Y, Chang Y, et al. Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay. Exp Mol Med. 2021;53:291-299 pubmed publisher
Kleinpeter P, Remy Ziller C, Winter E, Gantzer M, Nourtier V, Kempf J, et al. By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1. J Virol. 2019;93: pubmed publisher
Li J, Xu S, Zhao Y, Yu S, Ge L, Xu B. Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord. Mol Med Rep. 2018;18:4969-4977 pubmed publisher
He S, Wu C, Xiao J, Li D, Sun Z, Li M. Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis. Scand J Immunol. 2018;87:e12648 pubmed publisher
Qu D, Sun J, Zhao L, Wang Q, Shao Q, Kong B, et al. Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2. Cell Mol Immunol. 2016;13:615-27 pubmed publisher
Martner A, Wiktorin H, Lenox B, Ewald Sander F, Aydin E, Aurelius J, et al. Histamine promotes the development of monocyte-derived dendritic cells and reduces tumor growth by targeting the myeloid NADPH oxidase. J Immunol. 2015;194:5014-21 pubmed publisher
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, et al. Tumor-activated TCR??? T cells from gastric cancer patients induce the antitumor immune response of TCR??? T cells via their antigen-presenting cell-like effects. J Immunol Res. 2014;2014:593562 pubmed publisher
Cavel O, Shomron O, Shabtay A, Vital J, Trejo Leider L, Weizman N, et al. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor. Cancer Res. 2012;72:5733-43 pubmed publisher
Weissmüller S, Semmler L, Kalinke U, Christians S, Muller Berghaus J, Waibler Z. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood. 2012;119:6268-77 pubmed publisher
Tso G, Law H, Tu W, Chan G, Lau Y. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Stem Cells. 2010;28:939-54 pubmed publisher
El Fakhry Y, Alturaihi H, Diallo D, Merhi Y, Mourad W. Critical role of lipid rafts in CD154-mediated T cell signaling. Eur J Immunol. 2010;40:770-9 pubmed publisher
van der Vliet H, Wang R, Yue S, Koon H, Balk S, Exley M. Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells. J Immunol. 2008;180:7287-93 pubmed
Sakthivel P, Shively V, Kakoulidou M, Pearce W, Lefvert A. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J Intern Med. 2007;261:399-407 pubmed
Hock B, Patton W, Budhia S, Mannari D, Roberts P, McKenzie J. Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients. Leukemia. 2002;16:865-73 pubmed

product information

brand :

R&D Systems

master code :

MAB141

SKU :

MAB141-100

product name :

Human B7-2/CD86 Antibody

unit size :

100 ug (also 25 ug, 500 ug)

seo description :

The Human B7-2/CD86 Antibody from R&D Systems is a mouse monoclonal antibody to B7-2/CD86. This antibody reacts with human. The Human B7-2/CD86 Antibody has been validated for the following applications: ELISA Development,Flow Cytometry,Neutralization,Functional Assay,Immunocytochemistry,Western Blot,CyTOF-ready,Knockout Validated.

target :

B7-2/CD86

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Monoclonal

clone :

37301

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.5-2.5 ug/mL, CyTOF-ready, Knockout Validated

host :

Mouse

immunogen :

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-2/CD86, Ala23-His244, Accession # P42081

isotype :

IgG1

purity :

Protein A or G purified from ascites

species :

Human

specificity :

Detects human B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.

gene symbol :

CD86

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

Antibody validation :

Knockout/Knockdown

top caption :

Detection of Human B7-2/CD86 antibody by Western Blot.

accessionNumbers :

P42081

applications :

Western Blot,CyTOF-ready,Knockout Validated,ELISA Development,Flow Cytometry,Neutralization,Immunocytochemistry,Functional Assay

USD :

469 USD

alt names :

Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage