product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human NKG2D/CD314 Antibody
catalog :
MAB139-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
489 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
149810
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 105
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; loading ...; fig s4a
Jiang Z, Qin L, Tang Y, Liao R, Shi J, He B, et al. Human induced-T-to-natural killer cells have potent anti-tumour activities. Biomark Res. 2022;10:13 pubmed publisher
J xfc rgens M, Claus M, Wingert S, Niemann J, Picard L, Hennes E, et al. Chronic stimulation desensitizes β2-adrenergic receptor responses in natural killer cells. Eur J Immunol. 2024;54:e2451299 pubmed publisher
Sugawara S, Hueber B, Woolley G, Terry K, Kroll K, Manickam C, et al. Multiplex interrogation of the NK cell signalome reveals global downregulation of CD16 signaling during lentivirus infection through an IL-18/ADAM17-dependent mechanism. PLoS Pathog. 2023;19:e1011629 pubmed publisher
King L, Toffoli E, Veth M, Iglesias Guimarais V, Slot M, Amsen D, et al. A bispecific γδ T-cell engager targeting EGFR activates a potent Vγ9Vδ2 T cell-mediated immune response against EGFR-expressing tumors. Cancer Immunol Res. 2023;: pubmed publisher
Laufer Britva R, Keren A, Bertolini M, Ullmann Y, Paus R, Gilhar A. Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata. elife. 2023;12: pubmed publisher
Carmena Moratalla A, Carpentier Solorio Y, Lema xee tre F, Farzam Kia N, Da Cal S, Guimond J, et al. Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients. Mult Scler Relat Disord. 2023;71:104542 pubmed publisher
Yang C, Huang C, Hu C, Fang J, Chen T, Lin Y, et al. Immunophenotype and antitumor activity of cytokine-induced killer cells from patients with hepatocellular carcinoma. PLoS ONE. 2023;18:e0280023 pubmed publisher
Puzzolo M, Breccia M, Mariglia P, Colafigli G, Pepe S, Scalzulli E, et al. Immunomodulatory Effects of IFNα on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation. J Clin Med. 2022;11: pubmed publisher
Chimienti R, Baccega T, Torchio S, Manenti F, Pellegrini S, Cospito A, et al. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for β cell replacement. Cell Rep. 2022;40:111423 pubmed publisher
Belkahla S, Brualla J, Fayd Herbe De Maudave A, Falvo P, Allende Vega N, Constantinides M, et al. The metabolism of cells regulates their sensitivity to NK cells depending on p53 status. Sci Rep. 2022;12:3234 pubmed publisher
Allende Vega N, Marco Brualla J, Falvo P, Alexia C, Constantinides M, De Maudave A, et al. Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1). Sci Rep. 2022;12:1341 pubmed publisher
Wu H, Li K, Pan W, Guo M, Qiu D, He Y, et al. Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway. Int Immunopharmacol. 2022;104:108497 pubmed publisher
Westhoff Smith D, Chakravorty A, Hayes M, Hammerschmidt W, Sugden B. The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells. MBio. 2021;12:e0224321 pubmed publisher
Petillo S, Capuano C, Molfetta R, Fionda C, Mekhloufi A, Pighi C, et al. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition. Cell Death Dis. 2021;12:836 pubmed publisher
Duncan S, Nagar R, Damerow M, Yashunsky D, Buzzi B, Nikolaev A, et al. A Trypanosoma brucei β3 glycosyltransferase superfamily gene encodes a β1-6 GlcNAc-transferase mediating N-glycan and GPI anchor modification. J Biol Chem. 2021;297:101153 pubmed publisher
Zhong X, Liao H, Hu S, Luo K, Zhu H. The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells. Mol Cell Probes. 2021;59:101759 pubmed publisher
Seidel E, Dassa L, Schuler C, Oiknine Djian E, Wolf D, Le Trilling V, et al. The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing. PLoS Pathog. 2021;17:e1008807 pubmed publisher
Phatarpekar P, Overlee B, Leehan A, Wilton K, Ham H, Billadeau D. The septin cytoskeleton regulates natural killer cell lytic granule release. J Cell Biol. 2020;219: pubmed publisher
Diab M, Schmiedel D, Seidel E, Bacharach E, Mandelboim O. Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D. Viruses. 2020;12: pubmed publisher
Capellero S, Erriquez J, Melano C, Mesiano G, Genta S, Pisacane A, et al. Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer. Sci Rep. 2020;10:6478 pubmed publisher
Sasawatari S, Okamoto Y, Kumanogoh A, Toyofuku T. Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells. J Immunol. 2020;204:1373-1385 pubmed publisher
Capuano C, Pighi C, Maggio R, Battella S, Morrone S, Palmieri G, et al. CD16 pre-ligation by defucosylated tumor-targeting mAb sensitizes human NK cells to γc cytokine stimulation via PI3K/mTOR axis. Cancer Immunol Immunother. 2020;69:501-512 pubmed publisher
Oh E, Min B, Li Y, Lian C, Hong J, Park G, et al. Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect). Cancers (Basel). 2019;11: pubmed publisher
Gras Navarro A, Espedal H, Joseph J, Trachsel Moncho L, Bahador M, Gjertsen B, et al. Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival. Cancers (Basel). 2019;11: pubmed publisher
Wirsching H, Zhang H, Szulzewsky F, Arora S, Grandi P, Cimino P, et al. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma. JCI Insight. 2019;4: pubmed publisher
Wilton K, Overlee B, Billadeau D. NKG2D/DAP10 Signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity. J Cell Sci. 2019;: pubmed publisher
Muñoz D, Yannone S, Daemen A, Sun Y, Vakar Lopez F, Kawahara M, et al. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging. JCI Insight. 2019;5: pubmed publisher
Zhuang X, Long E. CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7+ Tumor Cells. Cancer Immunol Res. 2019;7:939-951 pubmed publisher
Amorim C, Carvalho N, Neto J, Santos S, Grassi M, Carvalho L, et al. The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers. J Immunol Res. 2019;2019:6574828 pubmed publisher
Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed publisher
Nam H, Cho Y, Lim J, Kim S, Kim H, Sim S, et al. Functional status of immune cells in patients with long-lasting type 2 diabetes mellitus. Clin Exp Immunol. 2018;194:125-136 pubmed publisher
Dassa L, Seidel E, Oiknine Djian E, Yamin R, Wolf D, Le Trilling V, et al. The Human Cytomegalovirus Protein UL148A Downregulates the NK Cell-Activating Ligand MICA To Avoid NK Cell Attack. J Virol. 2018;92: pubmed publisher
Prévost J, Richard J, Medjahed H, Alexander A, Jones J, Kappes J, et al. Incomplete Downregulation of CD4 Expression Affects HIV-1 Env Conformation and Antibody-Dependent Cellular Cytotoxicity Responses. J Virol. 2018;92: pubmed publisher
Lee Y, Heo W, Nam J, Jeung Y, Bae J. The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells. J Radiat Res. 2018;59:245-252 pubmed publisher
Dai Y, Liu H, Liu Y, Zhang Y, He W. EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances γδT-cell-mediated cytotoxicity via TCR and NKG2D. Immunology. 2018;: pubmed publisher
Matalon O, Ben Shmuel A, Kivelevitz J, Sabag B, Fried S, Joseph N, et al. Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1. EMBO J. 2018;37: pubmed publisher
López Cobo S, Pieper N, Campos Silva C, García Cuesta E, Reyburn H, Paschen A, et al. Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors. Oncoimmunology. 2018;7:e1392426 pubmed publisher
Brighton P, Maruyama Y, Fishwick K, Vrljicak P, Tewary S, Fujihara R, et al. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium. elife. 2017;6: pubmed publisher
Miyashita T, Miki K, Kamigaki T, Makino I, Tajima H, Nakanuma S, et al. Low-dose valproic acid with low-dose gemcitabine augments MHC class I-related chain A/B expression without inducing the release of soluble MHC class I-related chain A/B. Oncol Lett. 2017;14:5918-5926 pubmed publisher
Sharma N, Trinidad C, Trembath A, Markiewicz M. NKG2D Signaling between Human NK Cells Enhances TACE-Mediated TNF-α Release. J Immunol. 2017;199:2865-2872 pubmed publisher
Charpak Amikam Y, Kubsch T, Seidel E, Oiknine Djian E, Cavaletto N, Yamin R, et al. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20. Sci Rep. 2017;7:8661 pubmed publisher
Lee Y, Martin Orozco N, Zheng P, Li J, Zhang P, Tan H, et al. Inhibition of the B7-H3 immune checkpoint limits tumor growth by enhancing cytotoxic lymphocyte function. Cell Res. 2017;27:1034-1045 pubmed publisher
Vadstrup K, Galsgaard E, Jensen H, Lanier L, Ryan J, Chen S, et al. NKG2D ligand expression in Crohn's disease and NKG2D-dependent stimulation of CD8+ T cell migration. Exp Mol Pathol. 2017;103:56-70 pubmed publisher
Santaguida S, Richardson A, Iyer D, M Saad O, Zasadil L, Knouse K, et al. Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System. Dev Cell. 2017;41:638-651.e5 pubmed publisher
Cai X, Caballero Benítez A, Gewe M, Jenkins I, Drescher C, Strong R, et al. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells. Neoplasia. 2017;19:471-482 pubmed publisher
Djaoud Z, Guethlein L, Horowitz A, Azzi T, Nemat Gorgani N, Olive D, et al. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells. J Exp Med. 2017;214:1827-1841 pubmed publisher
Lin F, Dai C, Ge X, Tang W, Lin Y, Wang Y, et al. Prognostic significance and functional implication of immune activating receptor NKG2D in gastric cancer. Biochem Biophys Res Commun. 2017;487:619-624 pubmed publisher
Jensen H, Chen S, Folkersen L, Nolan G, Lanier L. EBI3 regulates the NK cell response to mouse cytomegalovirus infection. Proc Natl Acad Sci U S A. 2017;114:1625-1630 pubmed publisher
Espinoza J, Nguyen V, Ichimura H, Pham T, Nguyen C, Pham T, et al. A functional polymorphism in the NKG2D gene modulates NK-cell cytotoxicity and is associated with susceptibility to Human Papilloma Virus-related cancers. Sci Rep. 2016;6:39231 pubmed publisher
Niu C, Jin H, Li M, Zhu S, Zhou L, Jin F, et al. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and γδ T cell-mediated lysis in multiple myeloma. Oncotarget. 2017;8:5954-5964 pubmed publisher
Parry H, Stevens T, Oldreive C, Zadran B, McSkeane T, Rudzki Z, et al. NK cell function is markedly impaired in patients with chronic lymphocytic leukaemia but is preserved in patients with small lymphocytic lymphoma. Oncotarget. 2016;7:68513-68526 pubmed publisher
Zocchi M, Camodeca C, Nuti E, Rossello A, Ven xe8 R, Tosetti F, et al. ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing. Oncoimmunology. 2016;5:e1123367 pubmed publisher
Ke M, Wang H, Zhou Y, Li J, Liu Y, Zhang M, et al. SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice. Oncotarget. 2016;7:49509-49526 pubmed publisher
Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E. CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruses. 2017;33:93-100 pubmed publisher
Chitadze G, Lettau M, Luecke S, Wang T, Janssen O, Furst D, et al. NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human ?? T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors. Oncoimmunology. 2016;5:e1093276 pubmed
Bauman Y, Drayman N, Ben nun Shaul O, Vitenstein A, Yamin R, Ophir Y, et al. Downregulation of the stress-induced ligand ULBP1 following SV40 infection confers viral evasion from NK cell cytotoxicity. Oncotarget. 2016;7:15369-81 pubmed publisher
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hübscher D, et al. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE. 2015;10:e0125544 pubmed publisher
Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, et al. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer. 2015;15:351 pubmed publisher
Frishman Levy L, Shemesh A, Bar Sinai A, Ma C, Ni Z, Frenkel S, et al. Central nervous system acute lymphoblastic leukemia: role of natural killer cells. Blood. 2015;125:3420-31 pubmed publisher
Karimi M, Aguilar O, Zou B, Bachmann M, Carlyle J, Baldwin C, et al. A truncated human NKG2D splice isoform negatively regulates NKG2D-mediated function. J Immunol. 2014;193:2764-2771 pubmed publisher
Celis Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, et al. Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J. 2014;33:1928-40 pubmed publisher
Molfetta R, Quatrini L, Capuano C, Gasparrini F, Zitti B, Zingoni A, et al. c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells. Eur J Immunol. 2014;44:2761-70 pubmed publisher
Serrano Pertierra E, Cernuda Morollón E, Brdicka T, Hoøejši V, Lopez Larrea C. L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration. J Leukoc Biol. 2014;96:437-45 pubmed publisher
Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, et al. Natural killer cells induce eosinophil activation and apoptosis. PLoS ONE. 2014;9:e94492 pubmed publisher
Sangiolo D, Mesiano G, Gammaitoni L, Leuci V, Todorovic M, Giraudo L, et al. Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas. Cancer Res. 2014;74:119-29 pubmed publisher
Windheim M, Southcombe J, Kremmer E, Chaplin L, Urlaub D, Falk C, et al. A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions. Proc Natl Acad Sci U S A. 2013;110:E4884-93 pubmed publisher
Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W. Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXR? activation. J Exp Med. 2013;210:2675-92 pubmed publisher
Ho L, Yit P, Ng L, Linn Y, Zhao Y, Sun L, et al. The road to memory: an early rest for the long journey. J Immunol. 2013;191:5603-14 pubmed publisher
Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, et al. CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling. Eur J Immunol. 2013;43:2473-83 pubmed publisher
Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
Di Carlo E, Bocca P, Emionite L, Cilli M, Cipollone G, Morandi F, et al. Mechanisms of the antitumor activity of human V?9V?2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma. Mol Ther. 2013;21:1034-43 pubmed publisher
Ham H, Guerrier S, Kim J, Schoon R, Anderson E, Hamann M, et al. Dedicator of cytokinesis 8 interacts with talin and Wiskott-Aldrich syndrome protein to regulate NK cell cytotoxicity. J Immunol. 2013;190:3661-9 pubmed publisher
Donia M, Ellebaek E, Andersen M, Straten P, Svane I. Analysis of V?1 T cells in clinical grade melanoma-infiltrating lymphocytes. Oncoimmunology. 2012;1:1297-1304 pubmed
Brown A, Dobbie I, Alakoskela J, Davis I, Davis D. Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood. 2012;120:3729-40 pubmed publisher
Tsukerman P, Stern Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, et al. MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells. Cancer Res. 2012;72:5463-72 pubmed publisher
Jardine L, Hambleton S, Bigley V, Pagan S, Wang X, Collin M. Sensitizing primary acute lymphoblastic leukemia to natural killer cell recognition by induction of NKG2D ligands. Leuk Lymphoma. 2013;54:167-73 pubmed publisher
Morandi F, Prigione I, Airoldi I. Human TCR??+ T cells represent a novel target for IL-27 activity. Eur J Immunol. 2012;42:1547-52 pubmed publisher
Serrano Pertierra E, Cernuda Morollón E, Lopez Larrea C. Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation. Eur J Immunol. 2012;42:2142-51 pubmed publisher
Glässner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, et al. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab Invest. 2012;92:967-77 pubmed publisher
Nielsen N, Ødum N, Ursø B, Lanier L, Spee P. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE. 2012;7:e31959 pubmed publisher
Andresen L, Skovbakke S, Persson G, Hagemann Jensen M, Hansen K, Jensen H, et al. 2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation. J Immunol. 2012;188:1847-55 pubmed publisher
Zocchi M, Catellani S, Canevali P, Tavella S, Garuti A, Villaggio B, et al. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas. Blood. 2012;119:1479-89 pubmed publisher
Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, et al. Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D. Cancer Res. 2012;72:460-71 pubmed publisher
Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto M, et al. DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction. Blood. 2011;117:4778-86 pubmed publisher
Inoue T, Tashiro Y, Takeuchi M, Otani T, Tsuji Takayama K, Okochi A, et al. Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins. Int J Oncol. 2011;38:1299-306 pubmed publisher
Qin H, Lee I, Panagiotopoulos C, Wang X, Chu A, Utz P, et al. Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling. Diabetes. 2011;60:857-66 pubmed publisher
Angelini D, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, et al. NKG2A inhibits NKG2C effector functions of ?? T cells: implications in health and disease. J Leukoc Biol. 2011;89:75-84 pubmed publisher
Cabezón R, Sintes J, Llinàs L, Benitez Ribas D. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol Lett. 2011;134:167-73 pubmed publisher
Cho D, Shook D, Shimasaki N, Chang Y, Fujisaki H, Campana D. Cytotoxicity of activated natural killer cells against pediatric solid tumors. Clin Cancer Res. 2010;16:3901-9 pubmed publisher
Maniar A, Zhang X, Lin W, Gastman B, Pauza C, Strome S, et al. Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood. 2010;116:1726-33 pubmed publisher
Purdy A, Campbell K. SHP-2 expression negatively regulates NK cell function. J Immunol. 2009;183:7234-43 pubmed publisher
Markel G, Seidman R, Besser M, Zabari N, Ortenberg R, Shapira R, et al. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells. PLoS ONE. 2009;4:e5597 pubmed publisher
Cerboni C, Ardolino M, Santoni A, Zingoni A. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood. 2009;113:2955-64 pubmed publisher
Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, et al. Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int J Cancer. 2009;124:1594-604 pubmed publisher
Kwong K, Baskar S, Zhang H, Mackall C, Rader C. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J Mol Biol. 2008;384:1143-56 pubmed publisher
Verhoeven D, de Hooge A, Mooiman E, Santos S, ten Dam M, Gelderblom H, et al. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol Immunol. 2008;45:3917-25 pubmed publisher
Dulphy N, Haas P, Busson M, Belhadj S, Peffault De Latour R, Robin M, et al. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation. J Immunol. 2008;181:2227-37 pubmed
Tang K, He C, Zeng G, Wu J, Song G, Shi Y, et al. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem Biophys Res Commun. 2008;370:578-83 pubmed publisher
Kim Y, Han M, Broxmeyer H. 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells. Blood. 2008;111:1378-86 pubmed
Shi J, Tricot G, Garg T, Malaviarachchi P, Szmania S, Kellum R, et al. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood. 2008;111:1309-17 pubmed
Zhang H, Snyder K, Suhoski M, Maus M, Kapoor V, June C, et al. 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J Immunol. 2007;179:4910-8 pubmed
Roda Navarro P, Vales Gomez M, Chisholm S, Reyburn H. Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function. Proc Natl Acad Sci U S A. 2006;103:11258-63 pubmed
Hanabuchi S, Watanabe N, Wang Y, Wang Y, Ito T, Shaw J, et al. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL). Blood. 2006;107:3617-23 pubmed
Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner E, et al. BCR/ABL promotes dendritic cell-mediated natural killer cell activation. Cancer Res. 2005;65:6409-17 pubmed
Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina T, et al. Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. J Leukoc Biol. 2005;78:359-71 pubmed
product information
brand :
R&D Systems
master code :
MAB139
SKU :
MAB139-100
product name :
Human NKG2D/CD314 Antibody
unit size :
100 ug (also 25 ug, 500 ug)
seo description :
The Human NKG2D/CD314 Antibody from R&D Systems is a mouse monoclonal antibody to NKG2D/CD314. This antibody reacts with human,mouse. The Human NKG2D/CD314 Antibody has been validated for the following applications: Bioassay,Neutralization,ELISA Capture,Blocking,Blocking/Neutralizing,Stimulation,Cytotoxicity Assay,Flow Cytometry,Surface Plasmon Resonance,Functional Assay,Immunohistochemistry,Immunohistochemistry-Frozen,Immunocytochemistry,Western Blot,Costimulation of T Cells,CyTOF-ready.
target :
NKG2D/CD314
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
149810
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Neutralization, Costimulation of T Cells, CyTOF-ready
host :
Mouse
immunogen :
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human,Mouse
specificity :
Detects human NKG2D/CD314 in Western blots.
gene symbol :
KLRK1
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of NKG2D/CD314 antibody in Human PBMCs gated on CD3- cells antibody by Flow Cytometry.
applications :
Western Blot,Costimulation of T Cells,CyTOF-ready,Bioassay,Blocking,Blocking/Neutralizing,Stimulation,Cytotoxicity Assay,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Frozen,Surface Plasmon Resonance,Neutralization,Immunocytochemistry,Functional Assay,ELISA Capture
extended description :
Clone 149810 was used by HLDA to establish CD designation
USD :
489 USD
product details :
Clone 149810 was used by HLDA to establish CD designation
alt names :
CD314, CD314 antigen, D12S2489E, FLJ17759, FLJ75772, Killer cell lectin-like receptor subfamily K member 1, killer cell lectin-like receptor subfamily K, member 1, KLR, KLRK1, NK cell receptor D, NKG2-D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.