product summary
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company name :
R&D Systems
product type :
antibody
product name :
MICA Antibody
catalog :
MAB1300-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
438 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
159227
reactivity :
human
application :
western blot, flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
Joung J, Kirchgatterer P, Singh A, Cho J, Nety S, Larson R, et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun. 2022;13:1606 pubmed publisher
Petillo S, Capuano C, Molfetta R, Fionda C, Mekhloufi A, Pighi C, et al. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition. Cell Death Dis. 2021;12:836 pubmed publisher
Seidel E, Dassa L, Schuler C, Oiknine Djian E, Wolf D, Le Trilling V, et al. The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing. PLoS Pathog. 2021;17:e1008807 pubmed publisher
Seidel E, Dassa L, Kahlon S, Tirosh B, Halenius A, Seidel Malkinson T, et al. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain. Nat Commun. 2021;12:2061 pubmed publisher
Diab M, Schmiedel D, Seidel E, Bacharach E, Mandelboim O. Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D. Viruses. 2020;12: pubmed publisher
Chang S, Kim H, Park C. Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens. Cells. 2020;9: pubmed publisher
Anft M, Netter P, Urlaub D, Prager I, Schaffner S, Watzl C. NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production. Cell Mol Immunol. 2020;17:347-355 pubmed publisher
Muñoz D, Yannone S, Daemen A, Sun Y, Vakar Lopez F, Kawahara M, et al. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging. JCI Insight. 2019;5: pubmed publisher
Sakiyama M, Espinoza I, Reddy A, de Carlo F, Kumar A, Levenson A, et al. Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer. Exp Mol Pathol. 2019;108:173-182 pubmed publisher
Abruzzese M, Bilotta M, Fionda C, Zingoni A, Soriani A, Petrucci M, et al. The homeobox transcription factor MEIS2 is a regulator of cancer cell survival and IMiDs activity in Multiple Myeloma: modulation by Bromodomain and Extra-Terminal (BET) protein inhibitors. Cell Death Dis. 2019;10:324 pubmed publisher
Dassa L, Seidel E, Oiknine Djian E, Yamin R, Wolf D, Le Trilling V, et al. The Human Cytomegalovirus Protein UL148A Downregulates the NK Cell-Activating Ligand MICA To Avoid NK Cell Attack. J Virol. 2018;92: pubmed publisher
Lin D, Hiron T, O Callaghan C. Intragenic transcriptional interference regulates the human immune ligand MICA. EMBO J. 2018;37: pubmed publisher
Charpak Amikam Y, Kubsch T, Seidel E, Oiknine Djian E, Cavaletto N, Yamin R, et al. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20. Sci Rep. 2017;7:8661 pubmed publisher
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, et al. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells. Cell Death Dis. 2017;8:e2973 pubmed publisher
Raneros A, Puras A, Rodriguez R, Colado E, Bernal T, Anguita E, et al. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition. Oncotarget. 2017;8:31959-31976 pubmed publisher
Berhani O, Nachmani D, Yamin R, Schmiedel D, Bar On Y, Mandelboim O. Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5' Untranslated Region. J Immunol. 2017;198:3662-3670 pubmed publisher
Diab M, Glasner A, Isaacson B, Bar On Y, Drori Y, Yamin R, et al. NK-cell receptors NKp46 and NCR1 control human metapneumovirus infection. Eur J Immunol. 2017;47:692-703 pubmed publisher
Goto K, Annan D, Morita T, Li W, Muroyama R, Matsubara Y, et al. Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study. Sci Rep. 2016;6:38407 pubmed publisher
Son C, Lee H, Koh E, Shin D, Bae J, Yang K, et al. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells. Sci Rep. 2016;6:32470 pubmed publisher
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, et al. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol. 2015;195:736-48 pubmed publisher
Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Béziat V, et al. Coordinated expression of DNAM-1 and LFA-1 in educated NK cells. J Immunol. 2015;194:4518-27 pubmed publisher
Kim G, Ha G, Bae J, Oh S, Kim S, Kang C. Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells. Oncol Lett. 2015;9:1641-1646 pubmed
Nielsen M, Dyring Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, et al. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol. 2015;135:1311-1319 pubmed publisher
Nachmani D, Gutschner T, Reches A, Diederichs S, Mandelboim O. RNA-binding proteins regulate the expression of the immune activating ligand MICB. Nat Commun. 2014;5:4186 pubmed publisher
Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, et al. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol. 2014;193:950-60 pubmed publisher
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, et al. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol. 2014;192:5332-42 pubmed publisher
Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, et al. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathog. 2014;10:e1004058 pubmed publisher
Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, et al. MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathog. 2014;10:e1003963 pubmed publisher
Tsukerman P, Stern Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, et al. MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells. Cancer Res. 2012;72:5463-72 pubmed publisher
Glässner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, et al. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab Invest. 2012;92:967-77 pubmed publisher
Nielsen N, Ødum N, Ursø B, Lanier L, Spee P. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE. 2012;7:e31959 pubmed publisher
Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva Nilsson L. Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells. PLoS ONE. 2011;6:e16899 pubmed publisher
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, et al. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Oncogene. 2011;30:2622-32 pubmed publisher
Björkström N, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, et al. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J Exp Med. 2011;208:13-21 pubmed publisher
Cerboni C, Ardolino M, Santoni A, Zingoni A. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood. 2009;113:2955-64 pubmed publisher
Verhoeven D, de Hooge A, Mooiman E, Santos S, ten Dam M, Gelderblom H, et al. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol Immunol. 2008;45:3917-25 pubmed publisher
image
image 1 :
R&D Systems MAB1300-100 image 1
Detection of MICA antibody in K562 Human Cell Line antibody by Flow Cytometry.
product information
brand :
R&D Systems
master code :
MAB1300
SKU :
MAB1300-100
product name :
MICA Antibody
description :
The Human MICA Antibody from R&D Systems is a mouse monoclonal antibody to MICA. This antibody reacts with human. The Human MICA Antibody has been validated for the following applications: Western Blot, Flow Cytometry, Blockade of Receptor-ligand Interaction, CyTOF-ready, Knockout Validated.
target :
MICA
category :
Primary Antibodies
unit sizes :
100 ug (also 25 ug, 500 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
clonality :
Monoclonal
clone :
159227
concentration :
LYOPH
conjugate :
Unconjugated
host :
Mouse
immunogen :
BaF3 mouse pro-B cell line transfected with human MICA
isotype :
IgG2b
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human
specificity :
Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.
gene symbol :
MICA
endotoxin note :
<0.10 EU per 1 µg of the antibody by the LAL method.
applications :
Western Blot, Flow Cytometry, Blockade of Receptor-ligand Interaction, CyTOF-ready, Knockout Validated
USD :
438 USD
alt names :
FLJ60820, MGC111087, PERB11.1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.