product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Siglec-5/Siglec-14 Antibody
catalog :
MAB10721
quantity :
500 ug (also 25 ug)
price :
559 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
194128
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry
more info or order :
R&D Systems product webpage
citations: 10
Reference
Wielgat P, Wawrusiewicz Kurylonek N, Czarnomysy R, Rogowski K, Bielawski K, Car H. The Paired Siglecs in Brain Tumours Therapy: The Immunomodulatory Effect of Dexamethasone and Temozolomide in Human Glioma In Vitro Model. Int J Mol Sci. 2021;22: pubmed publisher
Tsai C, Riestra A, Ali S, Fong J, Liu J, Hughes G, et al. Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages. J Innate Immun. 2020;12:333-343 pubmed publisher
Suematsu R, Miyamoto T, Saijo S, Yamasaki S, Tada Y, Yoshida H, et al. Identification of lipophilic ligands of Siglec5 and -14 that modulate innate immune responses. J Biol Chem. 2019;294:16776-16788 pubmed publisher
Huang P, Low P, Wang I, Hsu S, Angata T. Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses. J Biol Chem. 2018;293:19645-19658 pubmed publisher
Förster K, Sass S, Ehrhardt H, Mous D, Rottier R, Oak P, et al. Early Identification of Bronchopulmonary Dysplasia Using Novel Biomarkers by Proteomic Screening. Am J Respir Crit Care Med. 2018;197:1076-1080 pubmed publisher
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini E, et al. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Sci Rep. 2016;6:37953 pubmed publisher
Izquierdo Useros N, Lorizate M, Puertas M, Rodriguez Plata M, Zangger N, Erikson E, et al. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides. PLoS Biol. 2012;10:e1001448 pubmed publisher
Gunnarsson P, Fornander L, Påhlsson P, Grenegård M. Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes. Inflamm Res. 2010;59:89-95 pubmed publisher
Carlin A, Chang Y, Areschoug T, Lindahl G, Hurtado Ziola N, King C, et al. Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5. J Exp Med. 2009;206:1691-9 pubmed publisher
Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M. Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 2006;20:1964-73 pubmed
product information
brand :
R&D Systems
master code :
MAB10721
SKU :
MAB10721
product name :
Human Siglec-5/Siglec-14 Antibody
unit size :
500 ug (also 25 ug)
seo description :
The Human Siglec-5/Siglec-14 Antibody from R&D Systems is a mouse monoclonal antibody to Siglec-5/Siglec-14. This antibody reacts with fungus,human. The Human Siglec-5/Siglec-14 Antibody has been validated for the following applications: Flow Cytometry,Immunoprecipitation,Western Blot,Neutralization,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),CyTOF-ready.
target :
Siglec-5/Siglec-14
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
194128
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Flow Cytometry 2.5 ug/10^6 cells, Neutralization 0.04-0.2 ug/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
host :
Mouse
immunogen :
Mouse myeloma cell line NS0-derived recombinant human Siglec-5/Siglec-14, Lys18-Thr434, Accession # O15389
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Fungus,Human
specificity :
Detects human Siglec-5/Siglec-14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
gene symbol :
SIGLEC5
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Siglec-5/14 antibody in Human Monocytes antibody by Flow Cytometry.
accessionNumbers :
O15389
applications :
ELISA Capture (Matched Antibody Pair),CyTOF-ready,Flow Cytometry,Immunoprecipitation,Western Blot,Neutralization,Immunocytochemistry
USD :
559 USD
alt names :
CD170, CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5, CD33L2, OBBP2, Siglec14, Siglec5
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.