product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human B7-H3 MAb (Clone 185504)
catalog :
MAB1027-100
quantity :
100 ug (also 25 ug, 500 ug)
price :
499 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
185504
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
R&D Systems product webpage
citations: 11
Reference
Genova C, Tasso R, Rosa A, Rossi G, Reverberi D, Fontana V, et al. Prognostic Role of Soluble and Extracellular Vesicle-Associated PD-L1, B7-H3 and B7-H4 in Non-Small Cell Lung Cancer Patients Treated with Immune Checkpoint Inhibitors. Cells. 2023;12: pubmed publisher
Oroujeni M, Bezverkhniaia E, Xu T, Liu Y, Plotnikov E, Karlberg I, et al. Evaluation of an Affibody-Based Binder for Imaging of Immune Check-Point Molecule B7-H3. Pharmaceutics. 2022;14: pubmed publisher
Liu J, Yang S, Cao B, Zhou G, Zhang F, Wang Y, et al. Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes. J Hematol Oncol. 2021;14:21 pubmed publisher
Yang S, Cao B, Zhou G, Zhu L, Wang L, Zhang L, et al. Targeting B7-H3 Immune Checkpoint With Chimeric Antigen Receptor-Engineered Natural Killer Cells Exhibits Potent Cytotoxicity Against Non-Small Cell Lung Cancer. Front Pharmacol. 2020;11:1089 pubmed publisher
Majzner R, Theruvath J, Nellan A, Heitzeneder S, Cui Y, Mount C, et al. CAR T Cells Targeting B7-H3, a Pan-Cancer Antigen, Demonstrate Potent Preclinical Activity Against Pediatric Solid Tumors and Brain Tumors. Clin Cancer Res. 2019;25:2560-2574 pubmed publisher
Sucker A, Zhao F, Real B, Heeke C, Bielefeld N, Maβen S, et al. Genetic evolution of T-cell resistance in the course of melanoma progression. Clin Cancer Res. 2014;20:6593-604 pubmed publisher
Maeda N, Yoshimura K, Yamamoto S, Kuramasu A, Inoue M, Suzuki N, et al. Expression of B7-H3, a potential factor of tumor immune evasion in combination with the number of regulatory T cells, affects against recurrence-free survival in breast cancer patients. Ann Surg Oncol. 2014;21 Suppl 4:S546-54 pubmed publisher
Ramsay A, Clear A, Fatah R, Gribben J. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood. 2012;120:1412-21 pubmed publisher
Crispen P, Sheinin Y, Roth T, Lohse C, Kuntz S, Frigola X, et al. Tumor cell and tumor vasculature expression of B7-H3 predict survival in clear cell renal cell carcinoma. Clin Cancer Res. 2008;14:5150-7 pubmed publisher
Tran C, Thacker S, Louie D, Oliver J, White P, Endres J, et al. Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3. J Immunol. 2008;180:2989-98 pubmed
Das S, Suarez G, Beswick E, Sierra J, Graham D, Reyes V. Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection. J Immunol. 2006;176:3000-9 pubmed
product information
master code :
MAB1027
SKU :
MAB1027-100
product name :
Human B7-H3 MAb (Clone 185504)
unit size :
100 ug (also 25 ug, 500 ug)
description :
The Human B7-H3 MAb (Clone 185504) from R&D Systems is a mouse monoclonal antibody to B7-H3/CD276. This antibody reacts with human. The Human B7-H3 MAb (Clone 185504) has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Immunoprecipitation,Western Blot,CyTOF-ready,Knockout Validated.
target :
B7-H3
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Monoclonal
clone :
185504
concentration :
LYOPH
conjugate :
Unconjugated
host :
Mouse
immunogen :
Mouse myeloma cell line NS0-derived recombinant human B7-H3, Leu29-Pro245, Accession # NP_079516
isotype :
IgG1
purity :
Protein A or G purified from hybridoma culture supernatant
species :
Human
specificity :
Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) B7-H1, rhB7-H2, rhB7-1, rhB7-2, or recombinant mouse PD-L2 is observed.
gene symbol :
CD276
Antibody validation :
Knockout/Knockdown
top caption :
Detection of Human B7-H3 antibody by Western Blot.
accessionNumbers :
NP_079516
applications :
Immunohistochemistry,Flow Cytometry,Immunoprecipitation,Western Blot,CyTOF-ready,Knockout Validated
USD :
499 USD
alt names :
B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.