Z-LR-AMC Fluorogenic Peptide Substrate | R&D Systems ES008 product information

product summary

company name :

R&D Systems

product type :

other

product name :

Z-LR-AMC Fluorogenic Peptide Substrate

catalog :

ES008

quantity :

10 mg

price :

221 USD

more info or order :

R&D Systems product webpage

citations: 9

Reference
Di Chio C, Starvaggi J, Totaro N, Previti S, Natale B, Cosconati S, et al. Development of Novel Peptidyl Nitriles Targeting Rhodesain and Falcipain-2 for the Treatment of Sleeping Sickness and Malaria. Int J Mol Sci. 2024;25: pubmed publisher
Sager T, Wolfarth M, Leonard S, Morris A, Porter D, Castranova V, et al. Role of engineered metal oxide nanoparticle agglomeration in reactive oxygen species generation and cathepsin B release in NLRP3 inflammasome activation and pulmonary toxicity. Inhal Toxicol. 2016;28:686-697 pubmed
Allan E, Yates R. Redundancy between Cysteine Cathepsins in Murine Experimental Autoimmune Encephalomyelitis. PLoS ONE. 2015;10:e0128945 pubmed publisher
Zhao L, Hua T, CROWLEY C, Ru H, Ni X, Shaw N, et al. Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage. Cell Res. 2014;24:344-58 pubmed publisher
Zeeuwen P, van Vlijmen Willems I, Cheng T, Rodijk Olthuis D, Hitomi K, Hara Nishimura I, et al. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J. 2010;24:3744-55 pubmed publisher
Cheng T, Hitomi K, van Vlijmen Willems I, de Jongh G, Yamamoto K, Nishi K, et al. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J Biol Chem. 2006;281:15893-9 pubmed
Cortesio C, Jiang W. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch Biochem Biophys. 2006;449:164-70 pubmed
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, et al. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev. 2006;20:543-56 pubmed
Johnson G, Jiang W. Characterization of cathepsin L secreted by Sf21 insect cells. Arch Biochem Biophys. 2005;444:7-14 pubmed

product information

master code :

ES008

SKU :

ES008

product name :

Z-LR-AMC Fluorogenic Peptide Substrate

unit size :

10 mg

target :

Protease Substrates

category :

Activity Assays

species :

Multi-Species

theoretical molecular weight :

578 Da

details of functionality :

The peptide substrate contains a highly fluorescent 7-amino-4-methyl coumarin (AMC) group that is efficiently quenched by the amide bond formed between its amino group and the carboxyl group of the Arg residue. It can be used to measure the activities of peptidases that are capable of cleaving this amide bond, causing an increase in fluorescence. It is an excellent substrate for many cathespins. For example, it has been used to assay following recombinant human or mouse cathespins (rhCathespins or rmCathespins) followed by Catalog # in parentheses: rhCathepsin B (953-CY), rmCathepsin B (965-CY), rhCathepsin L (952-CY), and rhCathepsin V (1080-CY).

applications :

Bioactivity

extended description :

Substrate for Cathepsin B, Cathepsin L, and Cathepsin V

USD :

221 USD

product details :

Substrate for Cathepsin B, Cathepsin L, and Cathepsin V

storage :

Samples are stable for up to twelve months from date of receipt at -20░ C to -70░ C. The substrate can be aliquoted and stored -20░ C to -70░ C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

more info or order :

R&D Systems product webpage