Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate | R&D Systems ES007 product information

product summary

company name :

R&D Systems

product type :

other

product name :

Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

catalog :

ES007

quantity :

1 mg

price :

281 USD

more info or order :

R&D Systems product webpage

citations: 19

Reference
Suresh V, Sheik D, Detomasi T, Zhao T, Zepeda T, Saladi S, et al. A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19. Biosensors (Basel). 2023;13: pubmed publisher
Torchia J, Tavares A, Carstensen L, Chen D, Huang J, Xiao T, et al. Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivo. Sci Adv. 2022;8:eabq6527 pubmed publisher
Liang W, Wijaya J, Wei H, Noble A, Mancl J, Mo S, et al. Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition. Nat Commun. 2022;13:1833 pubmed publisher
Castiglione G, Zhou L, Xu Z, Neiman Z, Hung C, Duh E. Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. PLoS Biol. 2021;19:e3001510 pubmed publisher
Shukla S, Cho K, Rustagi V, Gao X, Fu X, Zhang S, et al. "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry. Int J Mol Sci. 2021;22: pubmed publisher
Huang K, Lin M, Kuo T, Chen C, Lin C, Chou Y, et al. Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection. EMBO Mol Med. 2021;13:e12828 pubmed publisher
Lu J, Sun P. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. J Biol Chem. 2020;295:18579-18588 pubmed publisher
Lu J, Sun P. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. bioRxiv. 2020;: pubmed publisher
Hao K, Lei W, Wu H, Wu J, Yang Z, Yan S, et al. LncRNA-Safe contributes to cardiac fibrosis through Safe-Sfrp2-HuR complex in mouse myocardial infarction. Theranostics. 2019;9:7282-7297 pubmed publisher
Schrom E, Huber M, Aneja M, Dohmen C, Emrich D, Geiger J, et al. Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA. Mol Ther Nucleic Acids. 2017;7:350-365 pubmed publisher
Putko B, Wang Z, Lo J, Anderson T, Becher H, Dyck J, et al. Circulating levels of tumor necrosis factor-alpha receptor 2 are increased in heart failure with preserved ejection fraction relative to heart failure with reduced ejection fraction: evidence for a divergence in pathophysiology. PLoS ONE. 2014;9:e99495 pubmed publisher
Qiu Y, Shil P, Zhu P, Yang H, Verma A, Lei B, et al. Angiotensin-converting enzyme 2 (ACE2) activator diminazene aceturate ameliorates endotoxin-induced uveitis in mice. Invest Ophthalmol Vis Sci. 2014;55:3809-18 pubmed publisher
Riquelme C, Acuña M, Torrejón J, Rebolledo D, Cabrera D, Santos R, et al. ACE2 is augmented in dystrophic skeletal muscle and plays a role in decreasing associated fibrosis. PLoS ONE. 2014;9:e93449 pubmed publisher
Yan Y, Salazar T, Dominguez J, Nguyen D, Li Calzi S, Bhatwadekar A, et al. Dicer expression exhibits a tissue-specific diurnal pattern that is lost during aging and in diabetes. PLoS ONE. 2013;8:e80029 pubmed publisher
Bhatia K, Zimmerman M, Sullivan J. Sex differences in angiotensin-converting enzyme modulation of Ang (1-7) levels in normotensive WKY rats. Am J Hypertens. 2013;26:591-8 pubmed publisher
Tsuchiya S, Simmer J, Hu J, Richardson A, Yamakoshi F, Yamakoshi Y. Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp). J Bone Miner Res. 2011;26:220-8 pubmed publisher
Park S, Bivona B, Kobori H, Seth D, Chappell M, Lazartigues E, et al. Major role for ACE-independent intrarenal ANG II formation in type II diabetes. Am J Physiol Renal Physiol. 2010;298:F37-48 pubmed publisher
Bergin D, Greene C, Sterchi E, Kenna C, Geraghty P, Belaaouaj A, et al. Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway. J Biol Chem. 2008;283:31736-44 pubmed publisher
Lovren F, Pan Y, Quan A, Teoh H, Wang G, Shukla P, et al. Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis. Am J Physiol Heart Circ Physiol. 2008;295:H1377-84 pubmed publisher

product information

master code :

ES007

SKU :

ES007

product name :

Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

unit size :

1 mg

target :

Protease Substrates

category :

Activity Assays

purity :

>95%, by HPLC.

species :

Multi-Species

theoretical molecular weight :

1145.2 Da

details of functionality :

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for caspase-1/interleukin-converting enzyme (ICE) and angiotensin I-converting enzyme-2 (ACE-2) (Enari, M. et al ., 1996, Nature 380 :723; Vickers, C. et al ., 2002, J. Biol. Chem. 277 :14838). The cleavage sites by ICE and ACE-2 are the peptide bonds between Ala and Asp and between Pro and Lys, respectively.

applications :

Bioactivity

extended description :

Substrate for ACE-2 and Caspase-1

USD :

281 USD

product details :

Substrate for ACE-2 and Caspase-1

storage :

Samples are stable for up to twelve months from date of receipt at -20░ C to -70░ C. The substrate can be aliquoted and stored -20░ C to -70░ C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

more info or order :

R&D Systems product webpage