Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate | R&D Systems ES005 product information

product summary

company name :

R&D Systems

product type :

other

product name :

Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

catalog :

ES005

quantity :

1 mg

price :

281 USD

more info or order :

R&D Systems product webpage

citations: 18

Reference
Dalmasso C, Ahmed N, Ghuneim S, Cincinelli C, Leachman J, Giani J, et al. Obese Male Mice Exposed to Early Life Stress Display Sympathetic Activation and Hypertension Independent of Circulating Angiotensin II. J Am Heart Assoc. 2024;13:e029511 pubmed publisher
Mett J, Lauer A, Janitschke D, Griebsch L, Theiss E, Grimm H, et al. Medium-Chain Length Fatty Acids Enhance Aβ Degradation by Affecting Insulin-Degrading Enzyme. Cells. 2021;10: pubmed publisher
Lu J, Sun P. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. J Biol Chem. 2020;295:18579-18588 pubmed publisher
Hohl M, Mayr M, Lang L, Nickel A, Barallobre Barreiro J, Yin X, et al. Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice. J Biol Chem. 2020;295:12605-12617 pubmed publisher
Lu J, Sun P. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. bioRxiv. 2020;: pubmed publisher
Dalmasso C, Leachman J, Ensor C, Yiannikouris F, Giani J, Cassis L, et al. Female Mice Exposed to Postnatal Neglect Display Angiotensin II-Dependent Obesity-Induced Hypertension. J Am Heart Assoc. 2019;8:e012309 pubmed publisher
Hu T, Li S, Lu M, Zhang L, Chen B, Mao R, et al. Neuroprotection induced by Navβ2‑knockdown in APP/PS1 transgenic neurons is associated with NEP regulation. Mol Med Rep. 2019;20:2002-2011 pubmed publisher
Sylte I, Dawadi R, Malla N, von Hofsten S, Nguyen T, Solli A, et al. The selectivity of galardin and an azasugar-based hydroxamate compound for human matrix metalloproteases and bacterial metalloproteases. PLoS ONE. 2018;13:e0200237 pubmed publisher
Smith Carpenter J, Alper B. Functional requirement for human pitrilysin metallopeptidase 1 arginine 183, mutated in amyloidogenic neuropathy. Protein Sci. 2018;27:861-873 pubmed publisher
Lim C, Han J. The antioxidant xanthorrhizol prevents amyloid-?-induced oxidative modification and inactivation of neprilysin. Biosci Rep. 2018;38: pubmed publisher
Grimm M, Thiel A, Lauer A, Winkler J, Lehmann J, Regner L, et al. Vitamin D and Its Analogues Decrease Amyloid-? (A?) Formation and Increase A?-Degradation. Int J Mol Sci. 2017;18: pubmed publisher
Liu Z, Zhu H, Fang G, Walsh K, Mwamburi M, Wolozin B, et al. Characterization of insulin degrading enzyme and other amyloid-? degrading proteases in human serum: a role in Alzheimer's disease?. J Alzheimers Dis. 2012;29:329-40 pubmed publisher
Lan X, Xu J, Kiyota T, Peng H, Zheng J, Ikezu T. HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes. J Immunol. 2011;186:6925-32 pubmed publisher
Wang S, Wang R, Chen L, Bennett D, Dickson D, Wang D. Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain. J Neurochem. 2010;115:47-57 pubmed publisher
Wendt W, Schulten R, Stichel C, Lübbert H. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. J Neurochem. 2009;110:1931-41 pubmed publisher
Wösten van Asperen R, Lutter R, Haitsma J, Merkus M, van Woensel J, van der Loos C, et al. ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin. Eur Respir J. 2008;31:363-71 pubmed
Miners J, Verbeek M, Rikkert M, Kehoe P, Love S. Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid. J Neurosci Methods. 2008;167:229-36 pubmed
Wisner A, Dufour E, Messaoudi M, Nejdi A, Marcel A, Ungeheuer M, et al. Human Opiorphin, a natural antinociceptive modulator of opioid-dependent pathways. Proc Natl Acad Sci U S A. 2006;103:17979-84 pubmed

product information

master code :

ES005

SKU :

ES005

product name :

Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

unit size :

1 mg

target :

Protease Substrates

category :

Activity Assays

purity :

>95%, by HPLC.

species :

Multi-Species

theoretical molecular weight :

1388 Da

details of functionality :

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for endothelin-converting enzyme-1 (ECE-1) and neprilysin. The cleavage site by ECE-1 is the peptide bond between Ala and Phe (Johnson, G.D. and Ahn, K., 2000, Anal. Biochem. 286 :112). The substrate is derived from bradykinin. It is also an excellent substrate for ACE and active Cathepsin A and X/Z/P.

applications :

Bioactivity, Binding Activity

extended description :

Substrate for ECE-1, ACE, Cathepsin A, Cathepsin X/Z/P, Neprilysin, and Insulysin

USD :

281 USD

product details :

Substrate for ECE-1, ACE, Cathepsin A, Cathepsin X/Z/P, Neprilysin, and Insulysin

storage :

Samples are stable for up to twelve months from date of receipt at -20░ C to -70░ C. The substrate can be aliquoted and stored -20░ C to -70░ C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light

more info or order :

R&D Systems product webpage