This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human UbcH5a/UBE2D1 - Ubiquitin Charged Protein
catalog :
E2-800
quantity :
100 ug
price :
375 USD
citations: 1
Reference
Yu T, Gan S, Zhu Q, Dai D, Li N, Wang H, et al. Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24. Nat Commun. 2019;10:4353 pubmed publisher
product information
brand :
R&D Systems, powered by Boston Biochem
master code :
E2-800
SKU :
E2-800-100
product name :
Recombinant Human UbcH5a/UBE2D1 - Ubiquitin Charged Protein
description :
The Recombinant Human UbcH5a/UBE2D1 - Ubiquitin Charged Protein from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UbcH5a/UBE2D1 - Ubiquitin Charged Protein has been validated for the following applications: Bioactivity.
target :
UbcH5a / UBE2D1
category :
Proteins and Enzymes
unit size :
100 ug
buffer :
Approximately 0.43 mg/ml (25 µM) with respect to UBE2D1. Formulated in 50 mM HEPES pH 7.5, 50 mM NaCl, 1 mM TCEP
conjugate :
Unconjugated
main entrez gene id :
7321
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
species :
Human
theoretical molecular weight :
25 kDa (17 kDa UBE2D1, 8.6 kDa Ubiquitin)
gene symbol :
UBE2D1
details of functionality :
Pre-charged UBE2D1 may be added directly to in vitro reactions containing E3 Ubiquitin ligases and ligase substrates (if applicable)—no ATP, E1 enzyme, or extra Ubiquitin are required. Reaction conditions will need to be optimized for each specific application. Note: Reducing agents including dithiothreitol (DTT) or mercaptoethanol ( beta ME) may cause unintended thiolytic release of Ubiquitin from the complex—care must be taken if these compounds are present in buffers. We suggest using a thioester friendly reductant such as TCEP if possible.
catalog number base :
E2-800
accessionNumbers :
P51668, P0CG47
applications :
Bioactivity
source :
The Recombinant Human UbcH5a / UBE2D1 - Ubiquitin Charged Protein from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UbcH5a / UBE2D1 - Ubiquitin Charged Protein has been validated for the following applications: Bioactivity.
USD :
375 USD
alt names :
E2(17)KB1, Stimulator of Fe transportUBC4/5 homolog, UBC4/5, UBC5A, UbcH5, UbcH5A, UBCH5AEC 6.3.2.19, UBCH5SFT, UBE2D1, Ubiquitin carrier protein D1, ubiquitin-conjugating enzyme E2 D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast), Ubiquitin-protein ligase D1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.