Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF | R&D Systems E2-664 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF

catalog :

E2-664

quantity :

100 ug

price :

239 USD

citations: 14

Reference
Di Rienzo M, Antonioli M, Fusco C, Liu Y, Mari M, Orhon I, et al. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains. Sci Adv. 2019;5:eaau8857 pubmed publisher
Romagny S, Bouaouiche S, Lucchi G, Ducoroy P, Bertoldo J, Terenzi H, et al. S-Nitrosylation of cIAP1 Switches Cancer Cell Fate from TNFα/TNFR1-Mediated Cell Survival to Cell Death. Cancer Res. 2018;78:1948-1957 pubmed publisher
Yang C, Zang W, Tang Z, Ji Y, Xu R, Yang Y, et al. A20/TNFAIP3 Regulates the DNA Damage Response and Mediates Tumor Cell Resistance to DNA-Damaging Therapy. Cancer Res. 2018;78:1069-1082 pubmed publisher
Brickner J, Soll J, Lombardi P, Vågbø C, Mudge M, Oyeniran C, et al. A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair. Nature. 2017;551:389-393 pubmed publisher
Ma Q, Ruan H, Peng L, Zhang M, Gack M, Yao W. Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity. Proc Natl Acad Sci U S A. 2017;114:E8760-E8769 pubmed publisher
Kliza K, Taumer C, Pinzuti I, Franz Wachtel M, Kunzelmann S, Stieglitz B, et al. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry. Nat Methods. 2017;14:504-512 pubmed publisher
Lang X, Tang T, Jin T, Ding C, Zhou R, Jiang W. TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity. J Exp Med. 2017;214:459-473 pubmed publisher
Tripathi E, Smith S. Cell cycle-regulated ubiquitination of tankyrase 1 by RNF8 and ABRO1/BRCC36 controls the timing of sister telomere resolution. EMBO J. 2017;36:503-519 pubmed publisher
Lu W, Liu S, Li B, Xie Y, Izban M, Ballard B, et al. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer. Oncogene. 2017;36:1364-1373 pubmed publisher
Zhang X, Li C, Zhang L, Wu C, Han L, Jin G, et al. TRAF6 Restricts p53 Mitochondrial Translocation, Apoptosis, and Tumor Suppression. Mol Cell. 2016;64:803-814 pubmed publisher
Zhang Q, Karnak D, Tan M, Lawrence T, Morgan M, Sun Y. FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4. Mol Cell. 2016;61:419-433 pubmed publisher
Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, et al. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes. Mol Cell. 2013;49:1108-20 pubmed publisher
Smith C, Berry D, McGlade C. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci. 2013;126:1366-80 pubmed publisher
Shibata Y, Oyama M, Kozuka Hata H, Han X, Tanaka Y, Gohda J, et al. p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO. Nat Commun. 2012;3:1061 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E2-664

SKU :

E2-664-100

product name :

Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF

description :

The Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF has been validated for the following applications: Enzyme Activity.

target :

UBE2N (Ubc13) / Uev1a Complex

category :

Proteins and Enzymes

unit size :

100 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM Nacl, 10% Glycerol (v/v), 2 mM TCEP.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

species :

Human

theoretical molecular weight :

18 kDa (UBE2N) 21 kDa (Uev1a)

gene symbol :

UBE2N

details of functionality :

Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex concentration of 0.1-1 µM.

catalog number base :

E2-664

accessionNumbers :

P61088 (UBE2N) Q13404 (Uev1a)

applications :

Enzyme Activity

source :

The Recombinant Human His6-UBE2N (Ubc13) / Uev1a Complex, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human His6-UBE2N (Ubc13) / Uev1a Complex, CF has been validated for the following applications: Enzyme Activity.

USD :

239 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.