product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF
catalog :
E2-656-100
quantity :
100 ug
price :
355 USD
more info or order :
R&D Systems product webpage
citations: 7
Reference
Radko Juettner S, Yue H, Myers J, Carter R, Robertson A, Mittal P, et al. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF. Nature. 2024;628:442-449 pubmed publisher
Cathcart A, Bird G, Wales T, Herce H, Harvey E, Hauseman Z, et al. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer. Nat Chem Biol. 2020;16:1218-1226 pubmed publisher
Wolf E, Mabry A, Damania B, Mayo L. Mdm2-mediated neddylation of pVHL blocks the induction of antiangiogenic factors. Oncogene. 2020;39:5228-5239 pubmed publisher
Zhou H, Lu J, Liu L, Bernard D, Yang C, Fernandez Salas E, et al. A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation. Nat Commun. 2017;8:1150 pubmed publisher
Coleman K, Békés M, Chapman J, Crist S, Jones M, Ueberheide B, et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. elife. 2017;6: pubmed publisher
Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, et al. The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity. J Biol Chem. 2015;290:296-309 pubmed publisher
Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, et al. SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1). J Biol Chem. 2014;289:34728-42 pubmed publisher
product information
master code :
E2-656
SKU :
E2-656-100
product name :
Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF
unit size :
100 ug
description :
The Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF has been validated for the following applications: Enzyme Activity.
target :
UBE2M/Ubc12
category :
Proteins and Enzymes
buffer :
Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.
species :
Human, Mouse, Rat
theoretical molecular weight :
21 kDa
gene symbol :
UBE2M
details of functionality :
Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 ╡M.
accessionNumbers :
P61081.1
applications :
Enzyme Activity
source long :
E. coli -derived UBE2M/Ubc12 protein Met1 - Lys183
source short :
E. coli
USD :
355 USD
alt names :
EC 6.3.2, EC 6.3.2.-, hUbc12, NEDD8 carrier protein, NEDD8 protein ligase, UBC12, UBC12NEDD8-conjugating enzyme Ubc12, UbcH12, UBC-RS2, ubiquitin carrier protein M, Ubiquitin-conjugating enzyme E2 M, ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12), ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), ubiquitin-protein ligase M, yeast UBC12 homolog
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.