Recombinant Human UBE2I/Ubc9 Protein, CF | R&D Systems E2-645 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human UBE2I/Ubc9 Protein, CF

catalog :

E2-645

quantity :

100 ug

price :

239 USD

citations: 6

Reference
Sohn S, Hearing P. Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications. MBio. 2019;10: pubmed publisher
Ma X, Yang T, Luo Y, Wu L, Jiang Y, Song Z, et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. elife. 2019;8: pubmed publisher
Appikonda S, Thakkar K, Shah P, Dent S, Andersen J, Barton M. Cross-talk between chromatin acetylation and SUMOylation of tripartite motif-containing protein 24 (TRIM24) impacts cell adhesion. J Biol Chem. 2018;293:7476-7485 pubmed publisher
Yang J, Liu Y, Wang B, Lan H, Liu Y, Chen F, et al. Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939. BMC Mol Biol. 2017;18:23 pubmed publisher
Wang W, Yan L, Liu Z, Liu L, Lin J, Liu Z, et al. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells. Nat Commun. 2017;8:363 pubmed publisher
Sohn S, Hearing P. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation. Proc Natl Acad Sci U S A. 2016;113:6725-30 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E2-645

SKU :

E2-645-100

product name :

Recombinant Human UBE2I/Ubc9 Protein, CF

description :

The Recombinant Human UBE2I/Ubc9 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UBE2I/Ubc9 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

UBE2I / Ubc9

category :

Proteins and Enzymes

unit size :

100 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.

conjugate :

Unconjugated

main entrez gene id :

7329

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

species :

Human

theoretical molecular weight :

18 kDa

gene symbol :

UBE2I

details of functionality :

Recombinant Human UBE2I/Ubc9 is a member of the SUMO-conjugating (E2) enzyme family that receives SUMO from a SUMO-activating (E1) enzyme and subsequently conjugates SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 µM.

catalog number base :

E2-645

accessionNumbers :

P63279

applications :

Enzyme Activity

source :

The Recombinant Human UBE2I / Ubc9 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UBE2I / Ubc9 Protein, CF has been validated for the following applications: Enzyme Activity.

USD :

239 USD

alt names :

C358B7.1, EC 6.3.2, EC 6.3.2.-, P18, SUMO-1-protein ligase, SUMO-protein ligase, Ubc9, UBC9SUMO-conjugating enzyme UBC9, UBCE9, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, ubiquitin conjugating enzyme 9, Ubiquitin-conjugating enzyme E2 I, ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, Ubiquitin-protein ligase I

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.