Recombinant Human UBE2I/Ubc9 Protein, CF | R&D Systems E2-645-100 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human UBE2I/Ubc9 Protein, CF

catalog :

E2-645-100

quantity :

100 ug

more info or order :

R&D Systems product webpage

citations: 10

Reference
Wang X, Liu Z, Xu X, Wang X, Ming Z, Liu C, et al. KSHV hijacks the antiviral kinase IKKε to initiate lytic replication. PLoS Pathog. 2025;21:e1012856 pubmed publisher
Liu J, Ackermann L, Hoffmann S, G xe1 l Z, Hendriks I, Jain C, et al. Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation. Nat Struct Mol Biol. 2024;31:1355-1367 pubmed publisher
Liu Z, Wang X, Liu C, Deng H, Li W, Wang X, et al. The SUMO E3 ligase activity of ORF45 determines KSHV lytic replication. PLoS Pathog. 2022;18:e1010504 pubmed publisher
Liu Z, Liu C, Wang X, Li W, Zhou J, Dong P, et al. RSK1 SUMOylation is required for KSHV lytic replication. PLoS Pathog. 2021;17:e1010123 pubmed publisher
Zhu G, Harischandra D, Ghaisas S, Zhang P, Prall W, Huang L, et al. TRIM11 Prevents and Reverses Protein Aggregation and Rescues a Mouse Model of Parkinson's Disease. Cell Rep. 2020;33:108418 pubmed publisher
Sohn S, Hearing P. Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications. MBio. 2019;10: pubmed publisher
Ma X, Yang T, Luo Y, Wu L, Jiang Y, Song Z, et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. elife. 2019;8: pubmed publisher
Appikonda S, Thakkar K, Shah P, Dent S, Andersen J, Barton M. Cross-talk between chromatin acetylation and SUMOylation of tripartite motif-containing protein 24 (TRIM24) impacts cell adhesion. J Biol Chem. 2018;293:7476-7485 pubmed publisher
Yang J, Liu Y, Wang B, Lan H, Liu Y, Chen F, et al. Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939. BMC Mol Biol. 2017;18:23 pubmed publisher
Sohn S, Hearing P. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation. Proc Natl Acad Sci U S A. 2016;113:6725-30 pubmed publisher

product information

master code :

E2-645

SKU :

E2-645-100

product name :

Recombinant Human UBE2I/Ubc9 Protein, CF

unit size :

100 ug

description :

The Recombinant Human UBE2I/Ubc9 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human UBE2I/Ubc9 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

UBE2I/Ubc9

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES, NaCl, Glycerol and TCEP.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human

theoretical molecular weight :

18 kDa

gene symbol :

UBE2I

details of functionality :

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 ╡M.

accessionNumbers :

P63279.1

applications :

Enzyme Activity

source long :

E. coli -derived human UBE2I/Ubc9 protein Met1 - Ser158

source short :

E. coli

extended description :

Will be discontinued when existing inventory is gone.

product details :

Will be discontinued when existing inventory is gone.

alt names :

C358B7.1, EC 6.3.2, EC 6.3.2.-, P18, SUMO-1-protein ligase, SUMO-protein ligase, Ubc9, UBC9SUMO-conjugating enzyme UBC9, UBCE9, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, ubiquitin conjugating enzyme 9, Ubiquitin-conjugating enzyme E2 I, ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, Ubiquitin-protein ligase I

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage