Recombinant Human UbcH7/UBE2L3 Protein, CF | R&D Systems E2-640 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human UbcH7/UBE2L3 Protein, CF

catalog :

E2-640

quantity :

100 ug

price :

249 USD

citations: 11

Reference
Yin Q, Han T, Fang B, Zhang G, Zhang C, Roberts E, et al. K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signaling. Nat Commun. 2019;10:1870 pubmed publisher
Bosshard M, Aprigliano R, Gattiker C, Palibrk V, Markkanen E, Backe P, et al. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Sci Rep. 2017;7:15050 pubmed publisher
French M, Klosowiak J, Aslanian A, Reed S, Yates J, Hunter T. Mechanism of ubiquitin chain synthesis employed by a HECT domain ubiquitin ligase. J Biol Chem. 2017;292:10398-10413 pubmed publisher
Zhang X, Li B, Rezaeian A, Xu X, Chou P, Jin G, et al. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis. Nat Commun. 2017;8:14799 pubmed publisher
He S, Cao Y, Xie P, Dong G, Zhang L. The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function. Sci Rep. 2017;7:41364 pubmed publisher
Lin S, Lu S, Mulaj M, Fang B, Keeley T, Wan L, et al. Monoubiquitination Inhibits the Actin Bundling Activity of Fascin. J Biol Chem. 2016;291:27323-27333 pubmed publisher
Pan Y, Li R, Meng J, Mao H, Zhang Y, Zhang J. Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation. J Immunol. 2014;192:4758-64 pubmed publisher
Lonskaya I, Desforges N, Hebron M, Moussa C. Ubiquitination increases parkin activity to promote autophagic ?-synuclein clearance. PLoS ONE. 2013;8:e83914 pubmed publisher
Hebron M, Lonskaya I, Sharpe K, Weerasinghe P, Algarzae N, Shekoyan A, et al. Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6). J Biol Chem. 2013;288:4103-15 pubmed publisher
Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland J. ERα phosphorylation at Y537 by Src triggers E6-AP-ERα binding, ERα ubiquitylation, promoter occupancy, and target gene expression. Mol Endocrinol. 2012;26:1567-77 pubmed publisher
Varfolomeev E, Moradi E, Dynek J, Zha J, Fedorova A, Deshayes K, et al. Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J. 2012;447:427-36 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E2-640

SKU :

E2-640-100

product name :

Recombinant Human UbcH7/UBE2L3 Protein, CF

description :

The Recombinant Human UbcH7/UBE2L3 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UbcH7/UBE2L3 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

UbcH7 / UBE2L3

category :

Proteins and Enzymes

unit size :

100 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

conjugate :

Unconjugated

main entrez gene id :

7332

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

species :

Human

theoretical molecular weight :

18 kDa

gene symbol :

UBE2L3

details of functionality :

Recombinant Human UbcH7/UBE2L3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH7/UBE2L3 concentration of 0.1-1 µM.

catalog number base :

E2-640

accessionNumbers :

P68036

applications :

Enzyme Activity

source :

The Recombinant Human UbcH7 / UBE2L3 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UbcH7 / UBE2L3 Protein, CF has been validated for the following applications: Enzyme Activity.

USD :

249 USD

alt names :

E2-F1, EC 6.3.2.19, L-UBC, UBCE7, UbcH7, UBCH7UbcM4, UbcM4, UBE2L3, Ubiquitin carrier protein L3, ubiquitin-conjugating enzyme E2 L3, Ubiquitin-conjugating enzyme E2-F1, ubiquitin-conjugating enzyme E2L 3, ubiquitin-conjugating enzyme UBCH7, Ubiquitin-protein ligase L3

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.