product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human UbcH5b/UBE2D2 Protein, CF
catalog :
E2-622-100
quantity :
100 ug
price :
355 USD
more info or order :
citations: 45
Reference
Tang X, Mei Y, He K, Liu R, Lv X, Zhao Y, et al. The RING-type E3 ligase RIE1 sustains leaf longevity by specifically targeting AtACS7 to fine-tune ethylene production in Arabidopsis. Proc Natl Acad Sci U S A. 2024;121:e2411271121 pubmed publisher
Zhang R, Wu Y, Qu X, Yang W, Wu Q, Huang L, et al. The RING-finger ubiquitin E3 ligase TaPIR1 targets TaHRP1 for degradation to suppress chloroplast function. Nat Commun. 2024;15:6905 pubmed publisher
Chan A, Haley R, Najar M, González Martínez D, Bugaj L, Burslem G, et al. Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins. Nat Commun. 2024;15:5808 pubmed publisher
Radko Juettner S, Yue H, Myers J, Carter R, Robertson A, Mittal P, et al. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF. Nature. 2024;628:442-449 pubmed publisher
Wang J, Baddock H, Mafi A, Foe I, Bratkowski M, Lin T, et al. Structure of the p53 degradation complex from HPV16. Nat Commun. 2024;15:1842 pubmed publisher
Wu S, Hu C, Zhu C, Fan Y, Zhou J, Xia X, et al. The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato. Mol Plant. 2024;17:598-613 pubmed publisher
Sun J, Huang S, Lu Q, Li S, Zhao S, Zheng X, et al. UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean. Nat Commun. 2023;14:6262 pubmed publisher
Monda J, Ge X, Hunkeler M, Donovan K, Ma M, Jin C, et al. HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways. Cell Rep. 2023;42:112496 pubmed publisher
Sharma J, Mulherkar S, Chen U, Xiong Y, Bajaj L, Cho B, et al. Calpain activity is negatively regulated by a KCTD7-Cullin-3 complex via non-degradative ubiquitination. Cell Discov. 2023;9:32 pubmed publisher
Mevissen T, Prasad A, Walter J. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away. Cell Rep. 2023;42:112125 pubmed publisher
Bullones Bola xf1 os A, Araujo Garrido J, Fern xe1 ndez Garc xed a J, Romero F, Bernal Bayard J, Ramos Morales F. SNRPD2 Is a Novel Substrate for the Ubiquitin Ligase Activity of the Salmonella Type III Secretion Effector SlrP. Biology (Basel). 2022;11: pubmed publisher
Zhou Y, Zheng R, Liu S, Disoma C, Du A, Li S, et al. Host E3 ligase HUWE1 attenuates the proapoptotic activity of the MERS-CoV accessory protein ORF3 by promoting its ubiquitin-dependent degradation. J Biol Chem. 2022;298:101584 pubmed publisher
Zhou H, Zaher M, Walter J, Brown A. Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates. Nucleic Acids Res. 2021;49:13194-13206 pubmed publisher
Liu Y, Qin C, Rao Y, Ngo C, Feng J, Zhao J, et al. SARS-CoV-2 Nsp5 Demonstrates Two Distinct Mechanisms Targeting RIG-I and MAVS To Evade the Innate Immune Response. MBio. 2021;12:e0233521 pubmed publisher
Kokic G, Wagner F, Chernev A, Urlaub H, Cramer P. Structural basis of human transcription-DNA repair coupling. Nature. 2021;598:368-372 pubmed publisher
Liebl M, Moehlenbrink J, Becker H, Raddatz G, Abdeen S, Aqeilan R, et al. DAZAP2 acts as specifier of the p53 response to DNA damage. Nucleic Acids Res. 2021;49:2759-2776 pubmed publisher
Albert M, Brinkmann K, Pokrzywa W, Günther S, Kronke M, Hoppe T, et al. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage. Cell Death Dis. 2020;11:740 pubmed publisher
Cathcart A, Bird G, Wales T, Herce H, Harvey E, Hauseman Z, et al. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer. Nat Chem Biol. 2020;16:1218-1226 pubmed publisher
Kim C, Noda T, Kim H, Kim G, Park S, Na Y, et al. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis. Cell Rep. 2020;32:107950 pubmed publisher
Tauber M, Kreuz S, Lemak A, Mandal P, Yerkesh Z, Veluchamy A, et al. Alternative splicing and allosteric regulation modulate the chromatin binding of UHRF1. Nucleic Acids Res. 2020;48:7728-7747 pubmed publisher
Yoo Y, Park Y, Lee H, Oanh N, Cho M, Heo J, et al. Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis. Cell Death Dis. 2019;10:938 pubmed publisher
Ting T, Goralski M, Klein K, Wang B, Kim J, Xie Y, et al. Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15. Cell Rep. 2019;29:1499-1510.e6 pubmed publisher
Zhang L, Wang Z, Shi R, Zhu X, Zhou J, Peng B, et al. RNF126 Quenches RNF168 Function in the DNA Damage Response. Genomics Proteomics Bioinformatics. 2018;16:428-438 pubmed publisher
Chen X, He Z, Fu M, Wang Y, Wu H, Li X, et al. The E3 Ubiquitin Ligase Siah-1 Suppresses Avian Reovirus Infection by Targeting p10 for Degradation. J Virol. 2018;92: pubmed publisher
Bosshard M, Aprigliano R, Gattiker C, Palibrk V, Markkanen E, Backe P, et al. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Sci Rep. 2017;7:15050 pubmed publisher
Lian N, Liu X, Wang X, Zhou Y, Li H, Li J, et al. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation. Proc Natl Acad Sci U S A. 2017;114:12321-12326 pubmed publisher
Zhang B, Holmlund M, Lorrain S, Norberg M, Bako L, Fankhauser C, et al. BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance. elife. 2017;6: pubmed publisher
Fan Z, Chen J, Kuang J, Lu W, Shan W. The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress Response. Front Plant Sci. 2017;8:995 pubmed publisher
Tawo R, Pokrzywa W, Kevei E, Akyuz M, Balaji V, Adrian S, et al. The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover. Cell. 2017;169:470-482.e13 pubmed publisher
Xu X, Kathare P, Pham V, Bu Q, Nguyen A, Huq E. Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis. Development. 2017;144:1831-1840 pubmed publisher
Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
Zhu J, Lee K, Jewett K, Man H, Chung H, Tsai N. Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors. PLoS Genet. 2017;13:e1006634 pubmed publisher
Nazio F, Carinci M, Valacca C, Bielli P, Strappazzon F, Antonioli M, et al. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation. J Cell Biol. 2016;215:841-856 pubmed
Mulvaney K, Matson J, Siesser P, Tamir T, Goldfarb D, Jacobs T, et al. Identification and Characterization of MCM3 as a Kelch-like ECH-associated Protein 1 (KEAP1) Substrate. J Biol Chem. 2016;291:23719-23733 pubmed
Du L, LI Y, Fakih M, Wiatrek R, Duldulao M, Chen Z, et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun. 2016;7:12326 pubmed publisher
Banh R, Iorio C, Marcotte R, Xu Y, Cojocari D, Rahman A, et al. PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia. Nat Cell Biol. 2016;18:803-813 pubmed publisher
Raychaudhuri K, Chaudhary N, Gurjar M, D Souza R, Limzerwala J, Maddika S, et al. 14-3-3? Gene Loss Leads to Activation of the Epithelial to Mesenchymal Transition Due to the Stabilization of c-Jun Protein. J Biol Chem. 2016;291:16068-81 pubmed publisher
Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, et al. Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death. Cell Death Differ. 2016;23:333-46 pubmed publisher
Kim S, Park D, Lee D, Kim W, Jeong Y, Lee J, et al. Vaccinia-related kinase 2 mediates accumulation of polyglutamine aggregates via negative regulation of the chaperonin TRiC. Mol Cell Biol. 2014;34:643-52 pubmed publisher
Smith C, Berry D, McGlade C. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci. 2013;126:1366-80 pubmed publisher
Lu Y, Li J, Cheng D, Parameswaran B, Zhang S, Jiang Z, et al. The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation. J Biol Chem. 2012;287:41014-22 pubmed publisher
Sosin A, Burger A, Siddiqi A, Abrams J, Mohammad R, Al Katib A. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas. J Hematol Oncol. 2012;5:57 pubmed publisher
Varfolomeev E, Moradi E, Dynek J, Zha J, Fedorova A, Deshayes K, et al. Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J. 2012;447:427-36 pubmed publisher
Pelisch F, Pozzi B, Risso G, Muñoz M, Srebrow A. DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation. J Biol Chem. 2012;287:30789-99 pubmed publisher
Xin D, Liao S, Xie Z, Hann D, Steinle L, Boller T, et al. Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234. PLoS Pathog. 2012;8:e1002707 pubmed publisher
product information
master code :
E2-622
SKU :
E2-622-100
product name :
Recombinant Human UbcH5b/UBE2D2 Protein, CF
unit size :
100 ug
description :
The Recombinant Human UbcH5b/UBE2D2 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human UbcH5b/UBE2D2 Protein, CF has been validated for the following applications: Enzyme Activity.
target :
UbcH5b/UBE2D2
category :
Proteins and Enzymes
buffer :
Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.
species :
Human
theoretical molecular weight :
17 kDa
gene symbol :
UBE2D2
details of functionality :
Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5b/UBE2D2 concentration of 0.1-1 ╡M.
accessionNumbers :
P62837.1
applications :
Enzyme Activity
source long :
E. coli -derived human UbcH5b/UBE2D2 protein Met1 - Met147
source short :
E. coli
USD :
355 USD
alt names :
E2(17)KB2, EC 6.3.2.19, PUBC1, UBC4/5, UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5), UBC5B, UBCH4, UbcH5B, UBE2D2, Ubiquitin carrier protein D2, ubiquitin-conjugating enzyme E2 D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast), Ubiquitin-protein ligase D2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.